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Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
VARIACIÓN DEL AREA DE PARED CELULAR EN Pinus radiata D. DON
Se evaluó la variación del porcentaje (%) de área de pared celular de la madera de Pinus radiata D. Don, según la posición en el árbol y la procedencia según el tipo de suelo (zonas geográfica) en el que crecieron los árboles.
Ana María Fernandez, Linette Salvo
doaj
Background Interstitial fluid flow induced by loading has demonstrated to be an important mediator for regulating bone mass and morphology. It is shown that the fluid movement generated by the intramedullary pressure (ImP) provides a source for pressure ...
Qin Yi-Xian, Brink Peter, Lam Hoyan
doaj +1 more source
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source
Variación del área de pared celular en Pinus Radiata D. Don
En este trabajo se evaluó la variación del porcentaje (%) de Área de pared celular de la madera de pino radiata (Pinus radiata D. Don), según la posición en el Árbol (altura y diámetro)y la procedencia según el tipo de suelo (zonas geográficas) en el que
Ana María Fernández, Linette Salvo
doaj
The cell wall is not a target of currently used therapeutics as Mycobacterium are considered naturally resistant to most β-lactam antibiotics. Therefore, combinations of conventional antibiotics with antibiotic activity-enhancing compounds offer a ...
D. A. Ivanchenko, L. M. Hrytsenko
doaj +1 more source
How phagocytic cells kill bacteria: Lessons from a professional killer
How phagocytic cells ingest and kill bacteria has been studied for more than a century, but many questions remain unanswered. The study of the amoeba Dictyostelium discoideum brings new answers, and new questions. Professional phagocytic cells such as neutrophils and macrophages, as well as free‐living soil amoebae like Dictyostelium discoideum, employ
Otmane Lamrabet, Pierre Cosson
wiley +1 more source
An objective method to quantify visible cell walls in thin sections of rye dough has been developed using light microscopy. This method is based on the detection of Calcofluor-induced fluorescence of mixed-linked (1,3)(1,4)-β-glucan found in cell walls with a fluorescence microscope, and calculating the area of blue fluorescence in the microscope ...
Parkkonen, Teija +3 more
openaire +2 more sources
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
The molecular genetics of plant cell wall synthesis is a critical area of research with significant implications for biofuel production. The plant cell wall, a complex network of biopolymers including cellulose, hemicellulose, pectin, and ...
Jyoti Prakash Sahoo +5 more
doaj +1 more source

