Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Growth of microorganisms in a Martian regolith simulant at reduced water activity. [PDF]
Raghavendra JB +2 more
europepmc +1 more source
Discovery of long non-coding RNAs in Aspergillus flavus response to water activity, CO2 concentration, and temperature changes. [PDF]
Davati N, Ghorbani A.
europepmc +1 more source
Activities for Wetland Functions (title provided or enhanced by cataloger) [PDF]
These activities were designed to enhance the third chapter of a module about wetlands that covers flood and erosion control, fish and wildlife habitat, food webs, wetlands "supermarket", and recreation and aesthetics.
core
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Redefining Separator Design and Water Activity for High-Energy Zinc Batteries Using Covalent Organic Framework. [PDF]
Zhang K +12 more
europepmc +1 more source
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Effect of packaging materials on lycopene vitamin C and water activity of dried tomato (Lycopersicon esculentum Mill.) powder during storage. [PDF]
Dufera LT +3 more
europepmc +1 more source
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Effect of water activity on the mechanical glass transition and dynamical transition of bacteria. [PDF]
Sogabe T +4 more
europepmc +1 more source

