Results 141 to 150 of about 452,167 (280)

A subset of ipRGCs regulates both maturation of the circadian clock and segregation of retinogeniculate projections in mice [PDF]

, 2017
et al,, Gray, Paul A, Herzog, Erik D, VanDrunk, Cassandra   +3 more
core   +2 more sources

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Constant Illumination Boosts Rice Immunity via the Nicotinamide Mononucleotide Signaling Pathway: A Mechanistic Exploration. [PDF]

Rice (N Y)
Sun M, Wu X, Ding K, Song L, Zhou M, Su X, Ye Z, Tang M, Lu T, Qian H, Fu Z, Ni Y.   +11 more
europepmc   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Rhythmic Mechanisms Governing CAM Photosynthesis in <i>Kalanchoe fedtschenkoi</i>: High-Resolution Temporal Transcriptomics. [PDF]

Int J Mol Sci
Hu R, Jawdy S, Sreedasyam A, Lipzen A, Wang M, Ng V, Daum C, Keymanesh K, Liu D, Hu A, Pasha A, Provart NJ, Borland AM, Tschaplinski TJ, Tuskan GA, Schmutz J, Yang X.   +16 more
europepmc   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Circadian reprogramming by timed sodium intake reveals transcriptional pathways of daily salt handling in the colon. [PDF]

Sci Adv
Torimitsu T, Kinouchi K, Changizi Ashtiani K, Abdelkarim S, Sato T, Kozuma T, Yao N, Iwahara A, Kato K, Tonomura S, Nakamura T, Yokota K, Kurihara I, Baldi P, Itoh H, Hayashi K.   +15 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Morphofunctional State of the Liver Under Conditions of Three-Month Dark Deprivation: The Influence of Circadian Disruptions and Melatonin. [PDF]

Int J Mol Sci
Areshidze DA, Kozlova MA, Anurkina AI, Chernikov VP.   +3 more
europepmc   +1 more source