Results 111 to 120 of about 873,686 (310)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Measured Water Temperature Characteristics in a Pipeline Distribution System

, 2006
YesThis paper describes the design, development, deployment and performance assessment of a prototype system for monitoring the 'health' of a water distribution network based on the temperature distribution and time-dependent variations in temperature ...
Day, Andrew J., Wood, Alastair S., Widdop, Peter D., Mounce, Steve R., Khan, Asar, Machell, James   +5 more
core  

Multi-objective optimization of integrated water reuse systems at a cluster scale

, 2010
Population growth and higher standards of living have and will continue to drive an increasing demand for water supplies. Past practices have seen conventional water sources diminish which has lead to environmental degradation.
Brock, C., McGregor, C., Colsey, C., Russo, E., Maier, H., Dandy, S.   +5 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Heat Losses Evaluation for Domestic Hot Water Distribution Systems [PDF]

Bulletin of the Polytechnic Institute of Jassy: Constructions, Architechture Section, 2006
In sanitary systems assembly, domestic hot water distribution supply networks represent an important weight for energetically balance.par This paper presents, in an analytical and graphical manner, the computational tools needed for domestic hot water ...
Theodor Mateescu, Radu Hudişteanu
doaj  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Measures of water quality reliability and their relation to hydraulic performance of water distribution networks

, 2013
Supplying adequate amount of water with the required pressure which is also reasonably free from disease causing organisms and harmful compounds are the objectives of water distribution system.
Fisseha, Sosina Getachew
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Data Enabled Predictive Control for Water Distribution Systems Optimization

Water Resources Research
Recent developments in control theory, coupled with the growing availability of real‐time data, have paved the way for improved data‐driven control methodologies.
Gal Perelman, Avi Ostfeld
doaj   +1 more source