Results 91 to 100 of about 784,267 (315)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Gender Equality and Inclusion in Water Resources Management – Action Piece [PDF]

, 2022
This Action Piece is an evidence-based call to action to all actors to build more equitable, inclusive, and sustainable societies. Countless gender and inclusion strategies within the water management sector have been developed across the world by non ...
Grant, M
core  

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Floodplain of the Orlice River (Eastern Bohemia, Czech Republic): The Natural Centre of Biodiversity

Journal of Landscape Ecology, 2015
The article presents the main results of a field research carried out between 2012 and 2014. It is a comparison case study of vegetation diversity of pools and dead-end branches of the Orlice River in Eastern Bohemia, Czech Republic.
Vávra Michal
doaj   +1 more source

Using System Dynamics to Explore the Water Supply and Demand Dilemmas of a Small South African Municipality [PDF]

, 2014
This paper explores the challenges faced by small municipalities in providing water services in a developing-world context of increasing urban demand. The paper uses a case study of the Sundays River Valley Municipality (SRVM) in South Africa.
Palmer, C.G. (author), Brent, A.C. (author), Musango, J.K., Brent, A.C., Clifford Holmes, J.K., Musango, J.K. (author), Slinger, J.H. (author), Palmer, C.G., Clifford Holmes, J.K. (author), Slinger, J.H.   +9 more
core  

Building a System Dynamics Model to Support Water Management: A Case Study of the Semiarid Region in the Brazilian Northeast [PDF]

, 2019
In recent centuries, water consumption rates have more than doubled and the population growth rate is rising constantly. As a result, water scarcity is now one of the main problems to be faced, mainly in semiarid regions. In light of such a dilemma, this
Oz Sahin, Oliveira Esquerre, Karla Patricia, Sahin, Oz, Karla Oliveira Esquerre, Wanderbeg Correia de Araujo, de Araujo, Wanderbeg Correia   +5 more
core   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source