Results 171 to 180 of about 7,416,707 (356)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Fluxes of Energy and Water Vapour from Grazed Pasture on a Mineral Soil in the Waikato

, 2009
The eddy covariance (EC) technique was used to measure half hourly fluxes of energy and evaporation from 15 December 2007 to 30 November 2008 at the Scott Research Farm, located 7 km east of Hamilton.
Kuske, Tehani Janelle
core  

Synthesis of PNIPAAm-g-P4VP Microgel as Draw Agent in Forward Osmosis by RAFT Polymerization and Reverse Suspension Polymerization to Improve Water Flux. [PDF]

Molecules, 2022
Gao Y, Yao X, Jiang Q, Liao J, Chen Y, Yu R.   +5 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Evaporation in fen wetlands [PDF]

, 2011
Wicken Fen represents a remnant of the once extensive peat fenlands of East Anglia, which survived large-scale drainage efforts intended to bring land into agricultural production due to its importance within the local economy and subsequently as a site ...
Kelvin, J.
core  

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Development of a XGBJSO hybrid machine learning model for predicting heat transfer coefficient of water and Al2O3 nanofluid in flow boiling

Nanotechnology Reviews
A flow boiling examination of water along with a nanofluid composed of water-Al2O3 flowing through an annulus has been carried out. The examination was carried out for the mass flux, pressure, and heat flux.
Shekhar, Sambyo Koj, Prajapati Om Shankar, Dadhich Manish, Karuppusamy Loganathan, Abbas Mohamed   +5 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Water vapour flux patterns and precipitation at Sierra de Guadarrama mountain range (Spain)

, 2014
It is well known how mountains play a crucial role in the climate system and have very particular climate features compared to other regions. Sierra de Guadarrama is a part of the Iberian Peninsula Central System (Spain), a mountain range located in the ...
Yagüe Anguis, Carlos, Sánchez, Enrique, Durán Montejano, Luis, Rodríguez De Fonseca, María Belén   +3 more
core   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source