Results 151 to 160 of about 28,100 (309)

EU WATER POLICY: RESEARCH DEVELOPMENTS AND NEW MANAGEMENT TOOLS [PDF]

At the end of 2000, the European Commission published its Water Framework Directive (WFD) in the Official Journal of the European Communities (2000/60/EC).
la Jeunesse, Isabelle, Cogan, Valeria, Giupponi, Carlo   +2 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

On the Choice of Cost and Effectiveness Indicators in the Context of the European Water Policy [PDF]

The Water Framework Directive requires EU Member States to analyse economic impacts of the Directive's implementation. To reach a "good status", instruments have to be judged according to their cost-effectiveness.
Sinabell, Franz, Schmid, Erwin
core   +1 more source

Sources of uncertainty in DNA metabarcoding of whole communities: Implications for its use in biomonitoring

Methods in Ecology and Evolution
Advancements have been made in the use of DNA‐based methods for the detection of single species. However, the routine application of DNA‐based methods to monitor whole communities using a metabarcoding approach and derive ecosystem status continues to be
J. Iwan Jones, Amanda Arnold, Dominik Buchner, John F. Murphy, Jasmine N. Childress, Pedro Beja, Florian Altermatt, Kristian Meissner, Ian Adams, Meri Anderson, Yaisel J. Borrell, Andrew G. Briscoe, Kat Bruce, Cátia Chaves, Chris Conyers, Kristy Deiner, Vasco Elbrecht, Álvaro Fueyo, Tim Goodall, Javier Granero‐Castro, Cristina Hartmann‐Fatu, Eleanor Jones, Elvira Mächler, Filipa M. S. Martins, Sam McGreig, Lyndall Pereira‐da‐Conceicoa, James L. Pretty, Daniel S. Read, Ben Price, Florian Leese, Rosetta C. Blackman   +30 more
doaj   +1 more source

Salt marshes in the water Framework Directive

, 2005
The Water Framework Directive requires the description of reference conditions and good ecological statuses of several ecological parameters and multimetrics for assessment. One of these parameters is 'salt marshes'. Due to many changes (embankments in particular) within the water bodies no directly applicable reference conditions can be developed for ...
Dijkema, K.S., de Jong, D.J., Vreeken-Buijs, M.J., van Duin, W.E.   +3 more
openaire   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Large-scale Water-related Innovative Renewable Energy Projects and the Water Framework Directive: Legal Issues and Solutions [PDF]

, 2017
This article discusses two legal issues that relate to the conflict between the interest of protecting water quality under the Water Framework Directive (WFD), versus the interest of promoting the use of innovative water-related renewable energy, with ...
van Hees, S.R.W.
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source