Results 71 to 80 of about 208,595 (265)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Investigating water movements around a shallow shipwreck in Big Tub Harbour of Lake Huron: Implications for managing and preserving underwater shipwrecks. [PDF]

PLoS One, 2023
Flood B, Girihagama L, Wells MG, Valipour R, Semcesen P, Parker S.   +5 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Concerted conformational dynamics and water movements in the ghrelin G protein-coupled receptor. [PDF]

Elife, 2021
Louet M, Casiraghi M, Damian M, Costa MG, Renault P, Gomes AA, Batista PR, M'Kadmi C, Mary S, Cantel S, Denoyelle S, Ben Haj Salah K, Perahia D, Bisch PM, Fehrentz JA, Catoire LJ, Floquet N, Banères JL.   +17 more
europepmc   +1 more source

Quantifying women’s water polo overhead movement volumes using inertial measurement units and machine learning techniques: a cross-sectional study

Scientific Reports
Water polo upper limb external monitoring is not routinely used yet is important in modifying injury risk as shooting volume and intensity are associated with upper limb injury.
Marguerite H. King, Raimundo Sanchez, Kate Watson, Michelle D. Smith, Bill Vicenzino   +4 more
doaj   +1 more source

Water community networks and the appropriation of neoliberal practices: social technology, depoliticization, and resistance

Ecology and Society, 2019
In the 1990s, various attempts to privatize water services in Latin America came from international financial institutions. Several social movements emerged to protest against privatization, especially from community and indigenous organizations ...
Emilie Dupuits
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Water movement in porous materials: Part 2 - The separation of the components of water movement

British Journal of Applied Physics, 1963
Vapour conductivities kv estimated from hydrogen diffusion in porous solids of decreasing moisture content theta rise slowly from zero at saturation θT to a maximum near dryness, and then fall rapidly again to zero at complete dryness. Liquid conductivities kl, computed from pore size distributions, and estimated from previous water transport ...
openaire   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source