Results 71 to 80 of about 1,773,531 (318)

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Evaluation of heterotrophic plate and chromogenic agar colony counting in water quality laboratories

MethodsX, 2015
The enumeration of bacteria using plate-based counts is a core technique used by food and water microbiology testing laboratories. However, manual counting of bacterial colonies is both time and labour intensive, can vary between operators and also ...
Gary Hallas, Paul Monis
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

How reliable are estimates of trace contaminants in rivers based on monthly grab samples?

Environmental Sciences Europe
Background Water quality assessment must rely on representative samples, but obtaining them is increasingly challenging given the growing number and diversity of trace contaminants emitted into water bodies.
Nikolaus Weber, Jounes Lutterbach, Christine Hufnagl, Ernis Saracevic, Steffen Kittlaus, Katarina Kozlica, Radmila Milačič Ščančar, Jörg Krampe, Matthias Zessner, Ottavia Zoboli   +9 more
doaj   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Water chemistry Nanticoke area Lake Erie 1969 [PDF]

, 1970
Monthly evaluation of the water chemistry characteristics at eight buoy marked in the Nanticoke area of Lake Erie for the period April to December 1969 are presented. The survey area extends 2.4 km offshore and 8.8 km along the shore.
Palmer, M. D.
core  

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Influence of Returned Water from Sludge Treatment and Return Activated Sludge on 2-Methylisoborneol and Geosmin Behaviors and Concentrations during Wastewater Treatment

Journal of Water and Environment Technology
Compounds such as 2-methylisoborneol (2-MIB) and geosmin present in the final effluents of wastewater treatment plants may affect water treatment in downstream areas. To reduce the concentrations of 2-MIB and geosmin, we investigated the influence of the
Ko Hosoda, Mitsuharu Nishikawa, Yasutaka Yasui, Kento Iwatani, Tomoyuki Ohara, Syunichiro Fujiwara, Toshiharu Segawa   +6 more
doaj   +1 more source