Results 161 to 170 of about 346,500 (295)

Cost-Effectiveness of Alternative Environmental Policies for Reducing Non-Point Source Pollution in Public Water Supplies

This study predicts farmers' response to policy alternatives aimed at alleviating non-point source pollution problems in a municipal water supply in Pike County, Illinois.
Mafoua, Edouard, Hornbaker, Robert H.
core  

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The Water and Food Restriction and its Multiples Effects

Psicologia, 2010
The study of the various impacts of unconditioned establishing operations has become important in behavior analysis. To investigated the effects of food and water restrictions on the weight and the pattern of consumption of 24 Wistar rats (12 female and ...
Laura Rocha, Fernanda Meirelles, Tereza Maria Azevedo Pires Sério, Nilza Micheletto, Rafael Bornacina   +4 more
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Combined effects of calcium sources and water restriction on fruit yield and quality of 'Ataulfo' and 'Kent' mangoes. [PDF]

Front Plant Sci
Rodrigues LS, Souto AGL, Cavalcante ÍHL, Carvalho LHM, Henrique JCGS, Silva GJNE, de Paiva Neto VB, Mendonça RMN, Gheyi HR, de Lima GS, Batista RO, de Sá CH, da Silva WAO, Cunha VDL, de Sousa Neto AT.   +14 more
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Physiological and Metabolic Responses to Water Restriction in Ewes Under Semi-Arid Conditions. [PDF]

Vet Sci
Costa CJP, Araújo GGL, Magalhães ALR, de Andrade AP, Turco SHN, Matos MHT, Silva DCND, Araújo CA, Valença RL, Silva TGFD, Campos FS, Gois GC.   +11 more
europepmc   +1 more source