Results 101 to 110 of about 882,940 (266)

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Weak Solutions for a Second Order Dirichlet Boundary Value Problem on Time Scale

Journal of Applied Mathematics, 2013
We adopt the Leray-Schauder degree theory and critical point theory to consider a second order Dirichlet boundary value problem on time scales and obtain some existence theorems of weak solutions for the previous problem.
Wandong Lou
doaj   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Fractional weak discrepancy and interval orders

Discrete Applied Mathematics, 2009
In this paper the authors generalize their former results of the range of the fractional weak discrepancy function and of the fractional weak discrepancy of posets and certain forbidden configurations on the range of the \(\text{wd}_F\) function for semiorders (interval orders with no induced \(3 + 1\)) to interval orders with no \(n + 1\), where \(n ...
Alan Shuchat, Randy Shull, Ann N. Trenk
openaire   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Canceling Effects of Conjunctions Render Higher-order Mean Motion Resonances Weak

The Astrophysical Journal
Mean motion resonances (MMRs) are a key phenomenon in orbital dynamics. The traditional disturbing function expansion in celestial mechanics shows that, for coplanar systems at low eccentricities, p : p  −  q MMRs exhibit a clear hierarchy of strengths ...
Elizabeth K. Jones, Samuel Hadden, Supakrai Teekamongkol, Daniel Tamayo   +3 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Gravitational waves from first-order phase transitions: from weak to strong

Journal of High Energy Physics
We study the generation of gravitational waves (GWs) during a cosmological first-order phase transition (PT) using the recently introduced Higgsless approach to numerically simulate the fluid motion induced by the PT.
Chiara Caprini, Ryusuke Jinno, Thomas Konstandin, Alberto Roper Pol, Henrique Rubira, Isak Stomberg   +5 more
doaj   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Long‐Term Follow‐Up of Chemotherapy‐Associated Biological Aging in Women With Early Breast Cancer

Aging and Cancer, EarlyView.
Women threated with adjuvant chemotherapy for early breast cancer have sustained long‐term increase in p16INK4a,, a robust marker of cell senescence, suggesting a chemotherapy‐associated age acceleration. p16INK4a as well as other biomarkers may identify patients at greatest risk for senescence‐related diseases of aging.
Hyman B. Muss, Alexis C. Wardell, Allison M. Deal, Kirsten A. Nyrop, Allison Ross, Yara G. Abdou, Jeffrey D. Aldrich, Lisa A. Cary, E. Claire Dees, Katherine E. Reeder‐Hayes, Emily M. Ray, Natalia Mitin, Denis Tsygankov   +12 more
wiley   +1 more source