Results 91 to 100 of about 882,940 (266)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Lattice Homomorphisms Between Weak Orders
The Electronic Journal of Combinatorics, 2019 We classify surjective lattice homomorphisms $W\to W'$ between the weak orders on finite Coxeter groups. Equivalently, we classify lattice congruences $\Theta$ on $W$ such that the quotient $W/\Theta$ is isomorphic to $W'$. Surprisingly, surjective homomorphisms exist quite generally: They exist if and only if the diagram of $W'$ is obtained from ...
openaire +4 more sources
Weak homogeneity of lattice ordered groups [PDF]
Czechoslovak Mathematical Journal, 2007 zbMATH Open Web Interface contents unavailable due to conflicting licenses.
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FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Weak Galerkin finite element methods for fourth order singular perturbation problems
四川大学学报. 自然科学版, 2018 In this paper, we discuss the construction and analysis of the weak Galerkin (WG) finite element methods for the fourth order singular perturbation problems in two and three dimensions.
WANG Lin, LUO Kun, ZHANG Shi-Quan
doaj
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
FEBS Open Bio, EarlyView.Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Local Convergence Analysis of an Efficient Fourth Order Weighted-Newton Method under Weak Conditions
Annals of the West University of Timisoara: Mathematics and Computer Science, 2018 Local convergence analysis of a fourth order method considered by Sharma et. al in [19] for solving systems of nonlinear equations. Using conditions on derivatives upto the order five, they proved that the method is of order four.
Argyros Ioannis K., George Santhosh
doaj +1 more source