Results 121 to 130 of about 696,986 (308)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
On the weak strong uniqueness of Koch Tataru's solution for the Navier-Stokes equations
, 2014 We prove the weak strong uniqueness between Koch-Tataru's solution and Leray's weak solution for three-dimensional incompressible Navier-Stokes equations. (C) 2014 Elsevier Inc.
Zhang, Zhifei, Dong, Bo-Qing
core +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia +3 more
wiley +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
MathematicsThis paper is devoted to establishing multiplicity results of nontrivial weak solutions to the fractional p-Laplacian equations of the Kirchhoff–Schrödinger type with Hardy potentials.
Yun-Ho Kim
doaj +1 more source
Some results on the penalised nematic liquid crystals driven by multiplicative noise: weak solution and maximum principle. [PDF]
Stoch Partial Differ Equ, 2019 Brzeźniak Z +2 more
europepmc +1 more source
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Model and analysis for quasistatic frictional contact of a 2D elastic bar
Electronic Journal of Differential Equations, 2018 This article constructs and analyzes a mathematical model that describes the quasistatic evolution of a 2D elastic bar that may come in frictional contact with a deformable foundation.
Mircea Sofonea, Meir Shillor
doaj