Results 51 to 60 of about 14,315,169 (231)

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Main controlling factors of production and reasonable fracturing scale optimization of deep coalbed methane wells in Shenfu block, Ordos Basin

Shiyou shiyan dizhi
The production of deep coalbed methane (CBM) wells in the Shenfu block of Ordos Basin varies greatly, with a lack of understanding of the main controlling factors.
Lichun SUN, Jia LIU, Na LI, Xinze LI, Heng WEN   +4 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Stimulated Reservoir Volume Characterization and Optimum Lateral Well Spacing Study of Two-Well Pad: Midland Basin Case Study

Geofluids, 2020
This paper introduces a flow simulation-based reservoir modeling study of a two-well pad with long production history and identical completion parameters in the Midland Basin. The study includes building geologic model, history matching, well performance
Jaeyoung Park, Candra Janova
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Seismic Dynamic Response of Adjacent Oil Well Casings: Effect of Inter-Well Spacing

Buildings
With the intensive development of oil and gas fields, multi-well layouts with reduced inter-well spacing are increasingly adopted to improve production efficiency. Such configurations, however, may significantly enhance seismic interaction among adjacent
Minjing Chen, Wei Lu, Yanjun He, Keyu Duan, Zilong Li, Yang Liu, Zhan Qu   +6 more
doaj   +1 more source

A computational method of critical well spacing of CO2 miscible and immiscible concurrent flooding

Petroleum Exploration and Development, 2017
: Based on the theory of non-Darcy seepage, a mathematical model for CO2 miscible and immiscible concurrent flooding considering changes of oil viscosity and threshold pressure gradient of oil and CO2 is established.
Jie CHI, Binshan JU, Guangzhong LYU, Xing ZHANG, Jiabei WANG   +4 more
doaj   +1 more source