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Open source automated western blot processor
HardwareX, 2019 Researchers in the biomedical area are always involved in methodologies comprising several processes that are repetitive and time-consuming; these researchers can take advantage of this time for other more important things.
Jorge Bravo-Martinez
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BlotIt-Optimal alignment of Western blot and qPCR experiments. [PDF]
PLoS ONE, 2022 Biological systems are frequently analyzed by means of mechanistic mathematical models. In order to infer model parameters and provide a useful model that can be employed for systems understanding and hypothesis testing, the model is often calibrated on ...
Svenja Kemmer +4 more
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A Simple, Reproducible Procedure for Chemiluminescent Western Blot Quantification
Bio-Protocol, 2023 Western blotting is a universally used technique to identify specific proteins from a heterogeneous and complex mixture. However, there is no clear and common procedure to quantify the results obtained, resulting in variations due to the different ...
DANIEL CAÑADA GARCÍA, Juan Arévalo
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International Journal of Medical Students, 2023 BACKGROUND: Congenital toxoplasmosis is an infection caused by the Toxoplasma gondii a protozoa obligate intracellular parasite that appears after a mother´s primary infection occurs during pregnancy.
Sebastián Serna Rivera +5 more
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Journal of Advances in VetBio Science and Techniques, 2021 Western Blot tekniği, herhangi bir örnekten istenen tek bir proteini özgün bir şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir bir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik protein izoformlarının ...
Hatice Pelin ASLIM, Oya BULUT
openaire +4 more sources
A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples
Gels, 2022 Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA)
Dario Lucas Helbing +4 more
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Analyzing efficiency of a lentiviral shRNA knockdown system in human enteroids using western blot and flow cytometry. [PDF]
STAR ProtocWilson AP +6 more
europepmc +2 more sources
Frontiers in Immunology, 2023 IntroductionThe environmental bacterium Burkholderia pseudomallei causes the often fatal and massively underreported infectious disease melioidosis.
Gabriel E. Wagner +13 more
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Aptamer Validation by Western Blot–an overview
Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022 Western blot is the main and basic technique in cellular and molecular biology. The principle of the western blot is the isolation and detection of the target molecule usually from a cellular extract.
Lamees Jamal Talib +2 more
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