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Forensic Analysis of Synthetically Generated Western Blot Images [PDF]

open access: yesIEEE Access, 2022
The widespread diffusion of synthetically generated content is a serious threat that needs urgent countermeasures. As a matter of fact, the generation of synthetic content is not restricted to multimedia data like videos, photographs or audio sequences ...
Sara Mandelli   +10 more
doaj   +2 more sources

Western Blot

open access: yesJournal of Advances in VetBio Science and Techniques, 2021
Theoretical Background/Context A blot, in molecular biology, is the technique that involves the transfer of DNA, RNA and proteins from a gel to a membrane (nitrocellulose or PVDF). Proteins can be detected by western blot (immunoblot).
H. P. Aslim, O. Bulut
semanticscholar   +4 more sources

Aptamer-Based Western Blot for Selective Protein Recognition

open access: yesFrontiers in Chemistry, 2020
Selective protein recognition is critical in molecular biology techniques such as Western blotting and ELISA. Successful detection of the target proteins in these methods relies on the specific interaction of the antibodies, which often bring a high ...
Yao Wang   +7 more
doaj   +2 more sources

BlotIt-Optimal alignment of Western blot and qPCR experiments. [PDF]

open access: yesPLoS ONE, 2022
Biological systems are frequently analyzed by means of mechanistic mathematical models. In order to infer model parameters and provide a useful model that can be employed for systems understanding and hypothesis testing, the model is often calibrated on ...
Svenja Kemmer   +4 more
doaj   +2 more sources

The Prevalence and Significance of HTLV-I/II Seroindeterminate Western Blot Patterns

open access: yesViruses, 2011
Human T-lymphotropic virus type I (HTLV-I) infects an estimated 15–20 million persons worldwide. A number of diseases have been associated with the virus including adult T-cell leukemia (ATL), HTLV-associated myelopathy/tropical spastic paraparesis (HAM ...
Yoshimi Akahata   +2 more
doaj   +2 more sources

A critical path to producing high quality, reproducible data from quantitative western blot experiments

open access: yesScientific Reports, 2022
Western blotting experiments were initially performed to detect a target protein in a complex biological sample and more recently, to measure relative protein abundance.
Sean C. Taylor   +3 more
semanticscholar   +1 more source

Blind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them

open access: yesPLoS Biology, 2022
Western blotting is a standard laboratory method used to detect proteins and assess their expression levels. Unfortunately, poor western blot image display practices and a lack of detailed methods reporting can limit a reader’s ability to evaluate or ...
Cristina Kroon   +16 more
semanticscholar   +1 more source

Metanalysis of the Diagnostic Performance of Western Blot for the Early Diagnosis of Congenital Toxoplasmosis

open access: yesInternational Journal of Medical Students, 2023
BACKGROUND: Congenital toxoplasmosis is an infection caused by the Toxoplasma gondii a protozoa obligate intracellular parasite that appears after a mother´s primary infection occurs during pregnancy.
Sebastián Serna Rivera   +5 more
doaj   +1 more source

A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples

open access: yesGels, 2022
Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA)
Dario Lucas Helbing   +4 more
doaj   +1 more source

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