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Brief guide to western blot assays [PDF]
Molecules and Cells: Western blot is an essential method that detects specific proteins using antibodies, which is one of the most widely applied techniques for protein detection.
Seokjun G. Ha +3 more
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A Simple, Reproducible Procedure for Chemiluminescent Western Blot Quantification
Bio-Protocol, 2023 Western blotting is a universally used technique to identify specific proteins from a heterogeneous and complex mixture. However, there is no clear and common procedure to quantify the results obtained, resulting in variations due to the different ...
DANIEL CAÑADA GARCÍA, Juan Arévalo
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International Journal of Medical Students, 2023 BACKGROUND: Congenital toxoplasmosis is an infection caused by the Toxoplasma gondii a protozoa obligate intracellular parasite that appears after a mother´s primary infection occurs during pregnancy.
Sebastián Serna Rivera +5 more
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Journal of Advances in VetBio Science and Techniques, 2021 Western Blot tekniği, herhangi bir örnekten istenen tek bir proteini özgün bir şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir bir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik protein izoformlarının ...
Hatice Pelin ASLIM, Oya BULUT
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A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples
Gels, 2022 Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA)
Dario Lucas Helbing +4 more
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Frontiers in Immunology, 2023 IntroductionThe environmental bacterium Burkholderia pseudomallei causes the often fatal and massively underreported infectious disease melioidosis.
Gabriel E. Wagner +13 more
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Aptamer Validation by Western Blot–an overview
Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022 Western blot is the main and basic technique in cellular and molecular biology. The principle of the western blot is the isolation and detection of the target molecule usually from a cellular extract.
Lamees Jamal Talib +2 more
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Microfluidic Western blotting [PDF]
Proceedings of the National Academy of Sciences, 2012 Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ ...
Alex J, Hughes, Amy E, Herr
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Iranian Journal of Parasitology, 2022 Background: Echinococcosis is a common parasite with zoonotic character created by a small cestode, Echinococcus spp., and is an important public health problem in Turkey as well as all over the world. We aimed to investigate antibodies in serum samples
Banuçiçek Yücesan +3 more
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