Results 41 to 50 of about 3,169,439 (400)
Western Blotting: An Introduction [PDF]
, 2015 Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. This process involves the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979.
Biji T. Kurien +5 more
openaire +3 more sources
Single-cell western blotting [PDF]
Nature Methods, 2014 To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct ∼10(3) concurrent single-cell western blots (scWesterns) in ∼4 h. A microscope slide supporting a 30-μm-thick photoactive polyacrylamide gel enables western blotting: settling of single cells into microwells, lysis in situ, gel electrophoresis ...
Hughes, Alex J +5 more
openaire +5 more sources
Synergistic effects of targeted PI3K signaling inhibition and chemotherapy in liposarcoma. [PDF]
, 2014 While liposarcoma is the second most common soft tissue malignant tumor, the molecular pathogenesis in this malignancy is poorly understood. Our goal was therefore to expand the understanding of molecular mechanisms that drive liposarcoma and identify ...
Borger, Darrell R +12 more
core +7 more sources
Acute Ethanol Administration Rapidly Increases Phosphorylation of Conventional Protein Kinase C in Specific Mammalian Brain Regions in Vivo [PDF]
, 2007 Background Protein kinase C (PKC) is a family of isoenzymes that regulate a variety of functions in the central nervous system including neurotransmitter release, ion channel activity, and cell differentiation.
A. Leslie Morrow +17 more
core +3 more sources
Parasites & Vectors, 2018 Trichinellosis is a meat-borne zoonotic disease caused by parasites of the genus Trichinella. To date, 12 taxa have been described. The identification of Trichinella species is crucial in order to identify the possible source of infection, the ...
M. Gómez-Morales +5 more
semanticscholar +1 more source
Immunodetection of Adhesin Pili Protein 38.6 kDa K. pneumoniae Using Western Blot
Elkawnie: Journal of Islamic Science and Technology, 2022 : Klebsiella pneumoniae is a pathogen that causes infections in communities and hospitals. These bacteria have many virulence factors that play an important role in the pathogenicity of infection and antibiotic resistance.
Dini Agustina +2 more
doaj +1 more source
The DNA damage response promotes Polyomavirus JC infection by nucleus to cytoplasm NF-Kappa B activation. [PDF]
, 2017 Background: Infection of glial cells by human neurotropic polyomavirus JC (JCV), the causative agent of the CNS demyelinating disease progressive multifocal leukoencephalopathy (PML), rapidly inflicts damage to cellular DNA.
Bellizzi, Anna +5 more
core +1 more source
Iranian Journal of Microbiology, 2020 Background and Objectives: Bovine tuberculosis diagnosis is usually performed by various tests with specific limitations. Mycobacterium bovis culture filtrate contains antigenic proteins that could be used to improve the sensitivity of bovine ...
Malihe Honarmand Kashi +3 more
doaj +1 more source
Cloning and expression of P67 protein of Mycoplasma leachii [PDF]
Veterinary World, 2017 Aim: The present study was undertaken to clone, express and study the immunogenicity of P67 protein of Mycoplasma leachii. Materials and Methods: P67 gene was amplified from genomic DNA of M. leachii.
Sabarinath Thankappan +5 more
doaj +1 more source
Modulation of the unfolded protein response by tauroursodeoxycholic acid counteracts apoptotic cell death and fibrosis in a mouse model for secondary biliary liver fibrosis [PDF]
, 2017 The role of endoplasmic reticulum stress and the unfolded protein response (UPR) in cholestatic liver disease and fibrosis is not fully unraveled. Tauroursodeoxycholic acid (TUDCA), a hydrophilic bile acid, has been shown to reduce endoplasmic reticulum (
Bogaerts, Eliene +9 more
core +2 more sources