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Erasable Western Blots

2003
Western blotting (reviewed in 1-3; see also this vol., Chapter 24 ) refers to formation and detection of an antibody-antigen complex between an antibody and a polypeptide that is immobilized on derivatized paper.
S H, Kaufmann, J H, Shaper
openaire   +3 more sources

“Rainbow” Western Blotting

2009
The ''Rainbow western'' method permits detection of multiple antigens on a single protein blot. The procedure utilizes horseradish peroxidase (HRPase)-based detection with both a chemiluminescent and colorimetric substrate. In the ''Rainbow western'' procedure four different HRPase-colorimetric substrates that produce black, brown, red, and green ...
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Western Blot Techniques

2017
The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then ...
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Western Blot Analysis

2012
Electrophoresis and the following western blot analysis are indispensable to investigate biochemical changes in cells and tissues exposed to nanoparticles or nanomaterials. Proteins should be extracted from the cells and tissues using a proper method, especially when phosphorylated proteins are to be detected.
openaire   +2 more sources

The development of microfluidic-based western blotting: Technical advances and future perspectives

Journal of Chromatography A, 2023
Christopher T Desire   +2 more
exaly  

Western blotting analysis of proteins separated by agarose native gel electrophoresis

International Journal of Biological Macromolecules, 2021
Chiaki Sakuma   +2 more
exaly  

Western Blotting Analysis

2003
R J, Fido, A S, Tatham, P R, Shewry
openaire   +2 more sources

Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting

Research and Practice in Thrombosis and Haemostasis, 2020
Axel Rosell   +2 more
exaly  

Western Cross Blot

2000
P, Hammerl   +3 more
openaire   +2 more sources

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