Results 21 to 30 of about 667,007 (283)
Western blotting of native proteins from agarose gels
BioTechniques, 2022 We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma +10 more
doaj +1 more source
Regulation of Expression of CXC Chemokines in Cultured Hepatocytes [PDF]
Iranian Journal of Public Health, 2005 Chemokines are low molecular weight proteins (8-17 kDa). They are classified in four distinct groups as CXC, CC, CX3C and C. Depending on the presence or absence of a motif called ELR (Arg-Leu-Glu) before the first cysteine residue in their structure ...
GhH Hassanshahiraviz, AJ Dickson
doaj +2 more sources
Microfluidic Western blotting [PDF]
Proceedings of the National Academy of Sciences, 2012 Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ ...
Alex J, Hughes, Amy E, Herr
openaire +2 more sources
Antibody Purification from Western Blotting
Bio-Protocol, 2012 This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case ...
Lin Fang
doaj +1 more source
Journal of Advances in VetBio Science and Techniques, 2021 Western Blot tekniği, herhangi bir örnekten istenen tek bir proteini özgün bir şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir bir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik protein izoformlarının ...
Hatice Pelin ASLIM, Oya BULUT
openaire +3 more sources
Antibodies used for Western blotting.
, 2021 Antibodies used for Western blotting.
Bhawna Yadav (172612) +6 more
core +1 more source
Western Blotting for Staphylococcus aureus AgrA
Bio-Protocol, 2014 Staphylococcus aureus has a quorum sensing system to regulate the expression of various virulence factors, which is exerted by the agr locus that encodes agrBDCA and a regulatory RNA called RNAIII.
Chikara Kaito, Kazuhisa Sekimizu
doaj +1 more source
Virus Overlay Assay (Far-Western blotting)
Bio-Protocol, 2013 Virus overlay assay is a method to detect protein-protein interaction in vitro. We performed the virus overlay assay to identify the receptor proteins interacting with the infectious spleen and kidney necrosis virus (ISKNV).
Kun-Tong Jia +3 more
doaj +1 more source
Simple analysis of gel images with IOCBIO Gel
BMC Biology, 2023 Background Current solutions for the analysis of Western Blot images lack either transparency and reproducibility or can be tedious to use if one has to ensure the reproducibility of the analysis.
Jaak Kütt +9 more
doaj +1 more source
Semina: Ciências Agrárias, 2019 Objetivou-se com esse estudo avaliar a prevalência da Artrite Encefalite Caprina (CAE) em reprodutores dos estados do Maranhão, Ceará, Piauí, Alagoas, Sergipe, Rio Grande do Norte e Paraíba.
Maximiana Mesquita de Sousa +8 more
doaj +1 more source