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2017
Western blotting (WB) is a most common technique used to detect specific proteins in biological samples. It utilizes simple principles to separate proteins according to their size and to bind them to a support allowing their detection with a specific antibody. WB is a powerful technique to detect a single or a complex of proteins and to monitor changes
A. M. Gressner, O. A. Gressner
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Western blotting (WB) is a most common technique used to detect specific proteins in biological samples. It utilizes simple principles to separate proteins according to their size and to bind them to a support allowing their detection with a specific antibody. WB is a powerful technique to detect a single or a complex of proteins and to monitor changes
A. M. Gressner, O. A. Gressner
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2003
Western blotting (reviewed in 1-3; see also this vol., Chapter 24 ) refers to formation and detection of an antibody-antigen complex between an antibody and a polypeptide that is immobilized on derivatized paper.
Scott H. Kaufmann, Joel H. Shaper
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Western blotting (reviewed in 1-3; see also this vol., Chapter 24 ) refers to formation and detection of an antibody-antigen complex between an antibody and a polypeptide that is immobilized on derivatized paper.
Scott H. Kaufmann, Joel H. Shaper
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Western Blot Processing Optimization: The Perfect Blot
2021Western blot processing is a well-established procedure that includes protein extraction from tissues and cells, gel electrophoresis separation, transfer to a membrane, and immunodetection with specific antibodies. Here, we show that optimization of washing helps to maximize the specific interactions of antigens and antibodies.
David P. Chimento +2 more
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Analytical Biochemistry, 1987
A method for successfully removing primary and secondary antibodies from nitrocellulose blots while preserving the originally immobilized polypeptides was developed. Polypeptides were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and electrophoretically transferred to nitrocellulose. Nonspecific binding sites
Scott H. Kaufmann +3 more
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A method for successfully removing primary and secondary antibodies from nitrocellulose blots while preserving the originally immobilized polypeptides was developed. Polypeptides were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and electrophoretically transferred to nitrocellulose. Nonspecific binding sites
Scott H. Kaufmann +3 more
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Western Blotting of the Endocannabinoid System [PDF]
, 2016 Measuring expression levels of G protein-coupled receptors (GPCRs) is an important step for understanding the distribution, function, and regulation of these receptors. A common approach for detecting proteins from complex biological systems is Western blotting.
Jim Wager-Miller, Ken Mackie
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2007
Western blotting is one of the most commonly used biochemical techniques to detect a specific protein from a mixture of proteins such as tissue extracts. Antibodies to the specific antigen are used to detect the protein. The mixture of proteins is resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a membrane.
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Western blotting is one of the most commonly used biochemical techniques to detect a specific protein from a mixture of proteins such as tissue extracts. Antibodies to the specific antigen are used to detect the protein. The mixture of proteins is resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a membrane.
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2013
Western analysis of apolipoproteins, lipoproteins, and proteins involved in lipoprotein metabolism can be challenging due to their size, hydrophobic nature, and, in some cases, low abundance. Here we describe a Western blotting method that has been used successfully for many proteins involved in lipoprotein metabolism, as well as intact LDL or HDL ...
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Western analysis of apolipoproteins, lipoproteins, and proteins involved in lipoprotein metabolism can be challenging due to their size, hydrophobic nature, and, in some cases, low abundance. Here we describe a Western blotting method that has been used successfully for many proteins involved in lipoprotein metabolism, as well as intact LDL or HDL ...
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Valid application of western blotting [PDF]
Molecular Biology Reports, 2014 Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. However, the systematic errors in the application of western blotting analysis are frequently to be found, which may compromise the interpretation of results.
Zanping Han +4 more
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A rapid multicolor Western blot
Journal of Immunological Methods, 1988A multicolor Western blotting technique was developed, by which different kinds or different subtypes of interferon were identified with different colors on a single Western blot. This was achieved by sequentially applying different sets of probing antibodies, enzyme-conjugated developing antibodies and enzyme substrates to detect each of the two or ...
Sun-Qu Zhang, Nancy Lee, Douglas Testa
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