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2017
The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then ...
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The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then ...
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2009
The ''Rainbow western'' method permits detection of multiple antigens on a single protein blot. The procedure utilizes horseradish peroxidase (HRPase)-based detection with both a chemiluminescent and colorimetric substrate. In the ''Rainbow western'' procedure four different HRPase-colorimetric substrates that produce black, brown, red, and green ...
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The ''Rainbow western'' method permits detection of multiple antigens on a single protein blot. The procedure utilizes horseradish peroxidase (HRPase)-based detection with both a chemiluminescent and colorimetric substrate. In the ''Rainbow western'' procedure four different HRPase-colorimetric substrates that produce black, brown, red, and green ...
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2021
Protein-protein interactions play an important role in the proper functioning of the cell. Far-western blotting is a useful technique to study protein-protein interactions. In this method, proteins of interest are attached to a membrane and then probed with a non-antibody protein.
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Protein-protein interactions play an important role in the proper functioning of the cell. Far-western blotting is a useful technique to study protein-protein interactions. In this method, proteins of interest are attached to a membrane and then probed with a non-antibody protein.
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Journal of applied physiology, 2015
Reference proteins (RP) or the total protein (TP) loaded is used to correct for uneven loading and/or transfer in Western blotting. However, the signal sensitivity and the influence of physiological conditions may question the normalization methods ...
A. Vigelsø +5 more
semanticscholar +1 more source
Reference proteins (RP) or the total protein (TP) loaded is used to correct for uneven loading and/or transfer in Western blotting. However, the signal sensitivity and the influence of physiological conditions may question the normalization methods ...
A. Vigelsø +5 more
semanticscholar +1 more source
2012
Electrophoresis and the following western blot analysis are indispensable to investigate biochemical changes in cells and tissues exposed to nanoparticles or nanomaterials. Proteins should be extracted from the cells and tissues using a proper method, especially when phosphorylated proteins are to be detected.
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Electrophoresis and the following western blot analysis are indispensable to investigate biochemical changes in cells and tissues exposed to nanoparticles or nanomaterials. Proteins should be extracted from the cells and tissues using a proper method, especially when phosphorylated proteins are to be detected.
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Immunodetection of Proteins by Western Blotting
2003The technique of protein immunoblotting, more commonly known as "Western" blotting, was first described a decade ago by Towbin et al., (1), using electric current to transfer polypeptides from polyacrylamide gels to nitrocellulose, although several groups had previously reported the use of capillary forces to effect the transfer of DNA ("Southern ...
C J Henderson, C R Wolf
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2021
Every cell contains several proteins. How do we identify every protein in the cell? This question vexed many scientists until the introduction of the procedure known as protein blotting or Western blotting. Cells obtained from tissues or cells grown outside the human body in a plastic flask are first busted to release its contents.
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Every cell contains several proteins. How do we identify every protein in the cell? This question vexed many scientists until the introduction of the procedure known as protein blotting or Western blotting. Cells obtained from tissues or cells grown outside the human body in a plastic flask are first busted to release its contents.
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1986
This chapter provides an overview of the western blot analysis method, which allows the investigator to identify specific proteins resolved by SDS polyacrylamide gel electrophoresis by binding with specific antisera. Proteins resolved on an acrylamide gel are transferred to a nitrocellulose (NC) filter that is incubated with the antisera.
Mark D. Dibner +2 more
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This chapter provides an overview of the western blot analysis method, which allows the investigator to identify specific proteins resolved by SDS polyacrylamide gel electrophoresis by binding with specific antisera. Proteins resolved on an acrylamide gel are transferred to a nitrocellulose (NC) filter that is incubated with the antisera.
Mark D. Dibner +2 more
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