Results 21 to 30 of about 777 (150)
Cas9-Rep fusion tethers donor DNA in vivo and boosts the efficiency of HDR-mediated genome editing. [PDF]
Plant Biotechnol JSummary Genome editing based on the homology‐directed repair (HDR) pathway enables scar‐free and precise genetic manipulations. However, the low frequency of HDR hinders its application in plant genome editing. In this study, we engineered the fusion of Cas9 and a viral replication protein (Rep) as a molecular bridge to tether donor DNA in vivo, which ...
Zhou Z +7 more
europepmc +2 more sources
Corrigendum: Identification and validation of Quantitative Trait Loci for Wheat dwarf virus resistance in wheat (Triticum spp.) [PDF]
Frontiers in Plant Science, 2022 Anne-Kathrin Pfrieme +6 more
doaj +2 more sources
وقوع ویروسهای مهم گندم در استان خوزستان [PDF]
Majallah-i ḥifāẓat-i giyāhān, 2022 بیماریهای ویروسی در غلات به لحاظ اقتصادی از مهمترین عوامل بیماریزای این محصولات به حساب میآیند. در این مطالعه وقوع سه ویروس مهم در گندم به نامهای ویروس کوتولگی گندم (Wheat dwarf virus, WDV)، ویروس کوتولگی زرد جو (Barley yellow dwarf virus, BYDV ...
محسن حسنی +4 more
doaj +1 more source
Beyond a few bases: methods for large DNA insertion and gene targeting in plants. [PDF]
Plant JSUMMARY Genome editing technologies like CRISPR/Cas have greatly accelerated the pace of both fundamental research and translational applications in agriculture. However, many plant biologists are functionally limited to creating small, targeted DNA changes or large, random DNA insertions.
Vollen K, Alonso JM, Stepanova AN.
europepmc +2 more sources
Crop Protection, 2021 Abstract Colorimetric and real-time loop-mediated isothermal amplification (cr-LAMP) assays were developed targeting the coat protein region of genomic DNA of Wheat dwarf virus (WDV). The method was optimized relating to primer selection, determination of isothermal temperature, and determination of incubation duration.
Ali Çelik, Ali Ferhan Morca
openaire +1 more source
Virome identification in wheat in the Czech Republic using small RNA deep sequencing
Journal of Integrative Agriculture, 2020 High-throughput deep-sequencing technology and bioinformatics analysis of the small RNA (sRNA) population isolated from plants allows universal virus detection and complete virome reconstruction for a given sample.
Khushwant SINGH +4 more
doaj +1 more source
Tools and targets: The dual role of plant viruses in CRISPR–Cas genome editing
The Plant Genome, Volume 16, Issue 2, June 2023., 2023 Abstract The recent emergence of tools based on the clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR‐associated (Cas) proteins have revolutionized targeted genome editing, thus holding great promise to both basic plant science and precision crop breeding. Conventional approaches for the delivery of editing components rely
Mireia Uranga, José‐Antonio Daròs
wiley +1 more source
CRISPR/Cas‐based tools for the targeted control of plant viruses
Molecular Plant Pathology, Volume 23, Issue 11, Page 1701-1718, November 2022., 2022 Plant viruses pose a major threat to global food security. In this review we provide an overview of the use and limitations of CRISPR/Cas‐based technology for resistance against DNA and RNA viruses in plants. Abstract Plant viruses are known to infect most economically important crops and pose a major threat to global food security.
Gaëlle Robertson +2 more
wiley +1 more source
Plant Pathology, Volume 70, Issue 7, Page 1552-1561, September 2021., 2021 In the field, ryegrass was found to host cereal‐infecting viruses and its potential role as a reservoir for wheat dwarf virus was confirmed by transmission experiments using leafhoppers. Abstract Non‐crop plants such as grasses and volunteer plants are an inseparable part of the flora of crop fields and can influence virus incidence in crop plants. The
Elham Yazdkhasti +2 more
wiley +1 more source
Temperature affected transmission, symptom development and accumulation of Wheat dwarf virus
Plant Protection Science, 2018 One of the biotic agents of yellowing and stunting in wheat and barley cultivations is Wheat dwarf virus (WDV) which is naturally transmitted by the leafhopper Psammotettix alienus (Dahlbom).
Mohamad Hamed Ghodoum Parizipour +2 more
doaj +1 more source