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We performed reflectance measurements with a time-resolved white-light spectroscopy system to monitor concentrations changes in a two-layer liquid phantom with optical properties similar to human tissues. By varying the concentrations of three inks with different spectral features, we changed the absorption coefficient of the upper and lower layer to ...
GIUSTO, ARIANNA +7 more
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Sensors and Actuators B: Chemical, 2017
Abstract The simultaneous determination of pesticides chlorpyrifos, imazalil and thiabendazole in drinking water and wine samples with a biosensor based on White Light Reflectance Spectroscopy is presented. A competitive immunoassay format was implemented followed by reaction of immunoadsorbed anti-analyte specific antibody molecules with a secondary
Georgios Koukouvinos +2 more
exaly +2 more sources
Abstract The simultaneous determination of pesticides chlorpyrifos, imazalil and thiabendazole in drinking water and wine samples with a biosensor based on White Light Reflectance Spectroscopy is presented. A competitive immunoassay format was implemented followed by reaction of immunoadsorbed anti-analyte specific antibody molecules with a secondary
Georgios Koukouvinos +2 more
exaly +2 more sources
Talanta, 2017
A label-free biosensor based on white light reflectance spectroscopy for the determination of PSA as semen indicator in forensic samples is presented. The sensor is based on a two-step immunoassay which employs the same polyclonal anti-PSA antibody as capture and detection antibody followed by reaction with streptavidin as a signal enhancement step ...
Georgios Koukouvinos +2 more
exaly +3 more sources
A label-free biosensor based on white light reflectance spectroscopy for the determination of PSA as semen indicator in forensic samples is presented. The sensor is based on a two-step immunoassay which employs the same polyclonal anti-PSA antibody as capture and detection antibody followed by reaction with streptavidin as a signal enhancement step ...
Georgios Koukouvinos +2 more
exaly +3 more sources
Sensors and Actuators B: Chemical, 2015
Abstract A label-free immunosensor based on white-light reflectance spectroscopy for the determination of total- and free-PSA in human serum is presented. The sensor consists of a silicon die with an overlayer of transparent material. Thermally grown silicon dioxide layers with thicknesses ranging between 700 and 3000 nm proved to be the most ...
Panagiota Pétrou +2 more
exaly +2 more sources
Abstract A label-free immunosensor based on white-light reflectance spectroscopy for the determination of total- and free-PSA in human serum is presented. The sensor consists of a silicon die with an overlayer of transparent material. Thermally grown silicon dioxide layers with thicknesses ranging between 700 and 3000 nm proved to be the most ...
Panagiota Pétrou +2 more
exaly +2 more sources
Sensors and Actuators B: Chemical, 2019
Abstract A miniaturized optical immunosensor based on White Light Reflectance Spectroscopy (WLRS) for the rapid and label-free detection of aflatoxin M1 (AFM1) in milk samples has been developed. WLRS sensing system consists of the measurement set-up and the biochip.
Georgios Koukouvinos +2 more
exaly +2 more sources
Abstract A miniaturized optical immunosensor based on White Light Reflectance Spectroscopy (WLRS) for the rapid and label-free detection of aflatoxin M1 (AFM1) in milk samples has been developed. WLRS sensing system consists of the measurement set-up and the biochip.
Georgios Koukouvinos +2 more
exaly +2 more sources
Sensors and Actuators B: Chemical, 2018
Abstract The fast and accurate determination of C-reactive protein (CRP) in human whole blood samples employing a white light reflectance spectroscopy sensing platform is demonstrated. Each assay cycle was completed in 12 min, including 5-min reaction with the diluted whole blood samples, 3-min with the biotinylated anti-CRP antibody, and 4-min with ...
Georgios Koukouvinos +2 more
exaly +2 more sources
Abstract The fast and accurate determination of C-reactive protein (CRP) in human whole blood samples employing a white light reflectance spectroscopy sensing platform is demonstrated. Each assay cycle was completed in 12 min, including 5-min reaction with the diluted whole blood samples, 3-min with the biotinylated anti-CRP antibody, and 4-min with ...
Georgios Koukouvinos +2 more
exaly +2 more sources
Journal of Colloid and Interface Science, 2011
The monitoring of the antibacterial agent triclosan binding at nanomolar concentration from an aqueous solution by employing a well-packed monolayer with a predetermined single orientation made of specifically synthesized 2,3-dimethyl-6-(undec-10-enamide)-6-deoxy-β-cyclodextrin (DMBUA) on a silicon wafer (Si/SiO(2)) coated with a novolac resin is ...
Konstantinos Misiakos +2 more
exaly +3 more sources
The monitoring of the antibacterial agent triclosan binding at nanomolar concentration from an aqueous solution by employing a well-packed monolayer with a predetermined single orientation made of specifically synthesized 2,3-dimethyl-6-(undec-10-enamide)-6-deoxy-β-cyclodextrin (DMBUA) on a silicon wafer (Si/SiO(2)) coated with a novolac resin is ...
Konstantinos Misiakos +2 more
exaly +3 more sources
Biosensors and Bioelectronics, 2016
A dual-analyte assay for the simultaneous determination of C-reactive protein (CRP) and D-dimer in human blood plasma based on a white light interference spectroscopy sensing platform is presented. Measurement is accomplished in real-time by scanning the sensing surface, on which distinct antibody areas have been created, with a reflection probe used ...
Georgios, Koukouvinos +11 more
openaire +2 more sources
A dual-analyte assay for the simultaneous determination of C-reactive protein (CRP) and D-dimer in human blood plasma based on a white light interference spectroscopy sensing platform is presented. Measurement is accomplished in real-time by scanning the sensing surface, on which distinct antibody areas have been created, with a reflection probe used ...
Georgios, Koukouvinos +11 more
openaire +2 more sources
Real-time multi-analyte label-free detection of proteins by white light reflectance spectroscopy
SPIE Proceedings, 2014Label-free optical sensors are considered ideal for biomedical analysis since they provide the advantages of multiplex and real-time detection. They still suffer; however, from lower sensitivity and/or more sophisticated equipment as compared to indirect detection methods.
Panagiota Petrou +6 more
openaire +1 more source
Journal of Biophotonics, 2018
Nasopharyngeal cancer (NPC) is an endemic with high incidence in Southern China and Southeast Asia countries. Screening for NPC under conventional white light imaging (WLI) nasopharyngoscope examination remains a great clinical challenge due to its poor sensitivity.
Duo Lin +12 more
openaire +2 more sources
Nasopharyngeal cancer (NPC) is an endemic with high incidence in Southern China and Southeast Asia countries. Screening for NPC under conventional white light imaging (WLI) nasopharyngoscope examination remains a great clinical challenge due to its poor sensitivity.
Duo Lin +12 more
openaire +2 more sources

