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Rapid and sensitive label-free determination of aflatoxin M1 levels in milk through a White Light Reflectance Spectroscopy immunosensor

Sensors and Actuators B: Chemical, 2019
Abstract A miniaturized optical immunosensor based on White Light Reflectance Spectroscopy (WLRS) for the rapid and label-free detection of aflatoxin M1 (AFM1) in milk samples has been developed. WLRS sensing system consists of the measurement set-up and the biochip.
Dimitra Tsounidi   +7 more
openaire   +3 more sources

Simultaneous determination of CRP and D-dimer in human blood plasma samples with White Light Reflectance Spectroscopy

Biosensors and Bioelectronics, 2016
A dual-analyte assay for the simultaneous determination of C-reactive protein (CRP) and D-dimer in human blood plasma based on a white light interference spectroscopy sensing platform is presented. Measurement is accomplished in real-time by scanning the sensing surface, on which distinct antibody areas have been created, with a reflection probe used ...
Georgios, Koukouvinos   +11 more
openaire   +4 more sources

A label-free flow-through immunosensor for determination of total- and free-PSA in human serum samples based on white-light reflectance spectroscopy

Sensors and Actuators B: Chemical, 2015
Abstract A label-free immunosensor based on white-light reflectance spectroscopy for the determination of total- and free-PSA in human serum is presented. The sensor consists of a silicon die with an overlayer of transparent material. Thermally grown silicon dioxide layers with thicknesses ranging between 700 and 3000 nm proved to be the most ...
G. Koukouvinos   +6 more
openaire   +3 more sources

Rapid C-reactive protein determination in whole blood with a White Light Reflectance Spectroscopy label-free immunosensor for Point-of-Care applications

Sensors and Actuators B: Chemical, 2018
Abstract The fast and accurate determination of C-reactive protein (CRP) in human whole blood samples employing a white light reflectance spectroscopy sensing platform is demonstrated. Each assay cycle was completed in 12 min, including 5-min reaction with the diluted whole blood samples, 3-min with the biotinylated anti-CRP antibody, and 4-min with ...
Georgios Koukouvinos   +5 more
openaire   +3 more sources

Autofluorescence and white light imaging‐guided endoscopic Raman and diffuse reflectance spectroscopy for in vivo nasopharyngeal cancer detection

Journal of Biophotonics, 2018
Nasopharyngeal cancer (NPC) is an endemic with high incidence in Southern China and Southeast Asia countries. Screening for NPC under conventional white light imaging (WLI) nasopharyngoscope examination remains a great clinical challenge due to its poor sensitivity.
Duo Lin   +12 more
openaire   +4 more sources

White-light time-resolved reflectance spectroscopy for monitoring constituents concentrations in layered diffusive media

SPIE Proceedings, 2007
We performed reflectance measurements with a time-resolved white-light spectroscopy system to monitor concentrations changes in a two-layer liquid phantom with optical properties similar to human tissues. By varying the concentrations of three inks with different spectral features, we changed the absorption coefficient of the upper and lower layer to ...
GIUSTO, ARIANNA   +7 more
openaire   +6 more sources

Monitoring Absorption Changes in a Layered Diffusive Medium by White-Light Time-Resolved Reflectance Spectroscopy

IEEE Transactions on Instrumentation and Measurement, 2010
Diffuse spectroscopy of turbid media has assumed a crucial role in the characterization of biological tissues. In particular, broadband time-resolved optical spectroscopy allows the direct determination in a single measurement of both the optical parameters of the tissue and the concentration of its main constituents.
A. Giusto   +7 more
openaire   +2 more sources

Variable-angle ultraviolet total-internal-reflection fluorescence spectroscopy using a white excitation light source

Review of Scientific Instruments, 1995
An apparatus for variable-angle ultraviolet total internal reflection fluorescence (TIRF) spectroscopy is described. TIRF spectroscopy is an analytical technique based on the excitation of fluorescence from a sample by means of an exponentially decaying surface energy wave. The limited penetration depth of the excitation volume results in excitation of
M. Toriumi   +3 more
openaire   +1 more source

Real-time multi-analyte label-free detection of proteins by white light reflectance spectroscopy

SPIE Proceedings, 2014
Label-free optical sensors are considered ideal for biomedical analysis since they provide the advantages of multiplex and real-time detection. They still suffer; however, from lower sensitivity and/or more sophisticated equipment as compared to indirect detection methods.
Panagiota Petrou   +6 more
openaire   +1 more source

Stone/tissue differentiation during intracorporeal lithotripsy using diffuse white light reflectance spectroscopy: In vitro and clinical measurements

Lasers in Surgery and Medicine, 2014
Background and ObjectiveHolmium laser lithotripsy is the ‘gold standard’ for intracorporeal fragmentation of stones. However, there is a risk of damaging and perforating the ureter wall when the laser is accidentally fired while the fiber is in contact with tissue.
Birgit, Lange   +3 more
openaire   +2 more sources

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