Results 81 to 90 of about 3,112,739 (313)

RIPK4 function interferes with melanoma cell adhesion and metastasis

Molecular Oncology, EarlyView.
RIPK4 promotes melanoma growth and spread. RIPK4 levels increase as skin lesions progress to melanoma. CRISPR/Cas9‐mediated deletion of RIPK4 causes melanoma cells to form less compact spheroids, reduces their migratory and invasive abilities and limits tumour growth and dissemination in mouse models.
Norbert Wronski, Sławomir Lasota, Ewelina Madej, Anna A. Brożyna, Małgorzata Szczygieł, Agnieszka Harazin‐Lechowska, Jan Czerbniak, Janusz Rys, Jaroslaw Czyz, Agnieszka Wolnicka‐Glubisz   +9 more
wiley   +1 more source

The First Case of the Identification of a Microorganism Directly from Whole Blood Using MALDI-TOF Mass Spectrometry in an Onco-Hematological Pediatric Patient with Bloodstream Infection

Antibiotics
Background: Bloodstream infections affect up to 20% of pediatric cancer patients receiving intensive care, contributing significantly to morbidity and mortality, with infection-related mortality rates reported to be as high as 16%.
Venere Cortazzo, Maria del Carmen Pereyra Boza, Vanessa Tuccio Guarna Assanti, Gianluca Foglietta, Gianluca Vrenna, Marilena Agosta, Elena Chaiter, Martina Rossitto, Barbara Lucignano, Manuela Onori, Valeria Fox, Marco Becilli, Pietro Merli, Filippo Frioni, Carlo Federico Perno, Paola Bernaschi   +15 more
doaj   +1 more source

Gas diffusion liquid storage bag and method of use for storing blood [PDF]

, 1979
The shelf life of stored whole blood may be doubled by adding a buffer which maintains a desired pH level. However, this buffer causes the generation of CO2 which, if not removed at a controlled rate, causes the pH value of the blood to decrease, which ...
Bank, H., Cleland, E. L.
core   +1 more source

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Enhancing bloodstream infection diagnostics: a novel filtration and targeted next-generation sequencing approach for precise pathogen identification

Frontiers in Microbiology
Bloodstream infections (BSIs) pose a significant diagnostic challenge, largely due to the limitations of traditional methods such as blood cultures. These methods often yield low positive rates, have lengthy processing times that delay treatment, and are
Ting-Syuan Lin, Ting-Syuan Lin, Ting-Syuan Lin, ZiHao Zhu, ZiHao Zhu, ZiHao Zhu, XiaoHong Lin, Hsi-Yuan Huang, Hsi-Yuan Huang, Hsi-Yuan Huang, Li-Ping Li, Li-Ping Li, Li-Ping Li, Jing Li, Jing Li, Jing Li, Jie Ni, Jie Ni, Jie Ni, PeiZhi Li, LanChun Chen, WeiXin Tang, HuiXin Liu, XiaoLong Se, MingFei Xie, Canling Long, Chih-Min Chiu, Szu-Han Fang, JiaMing Zhao, Yang-Chi-Dung Lin, Yang-Chi-Dung Lin, Yang-Chi-Dung Lin, XueTao Yu, Hsien-Da Huang, Hsien-Da Huang, Hsien-Da Huang, Hsien-Da Huang   +36 more
doaj   +1 more source

PCR Targeting Plasmodium Mitochondrial Genome of DNA Extracted from Dried Blood on Filter Paper Compared to Whole Blood. [PDF]

, 2014
Monitoring mortality and morbidity attributable to malaria is paramount to achieve elimination of malaria. Diagnosis of malaria is challenging and PCR is a reliable method for identifying malaria with high sensitivity.
A Diallo, A Farnert, AM Dondorp, AP Phyo, B Morassin, Bjørn Blomberg, C Fancony, C Haanshuus, C Wongsrichanalai, CJ Murray, CV Plowe, E Bottius, GE Strom, Gro EA Strøm, H Reyburn, J Hwang, J Wooden, K Moll, LC Okell, LN Wangai, M Pritsch, Maulidi Fataki, N Valecha, NB Gadalla, Nina Langeland, RJ Pearce, S Ataei, S Chaorattanakawee, S Proux, S Shekalaghe, SA Al-Harthi, Sabrina Moyo, T Hanscheid, WHO, WHO   +34 more
core   +3 more sources

Somatic mutational landscape in von Hippel–Lindau familial hemangioblastoma

Molecular Oncology, EarlyView.
The causes of central nervous system (CNS) hemangioblastoma in Von Hippel–Lindau (vHL) disease are unclear. We used Whole Exome Sequencing (WES) on familial hemangioblastoma to investigate events that underlie tumor development. Our findings suggest that VHL loss creates a permissive environment for tumor formation, while additional alterations ...
Maja Dembic, Anne Nørremølle, Lilian Bomme Ousager, Lars van Brakel Andersen, Marie Louise Mølgaard Binderup, Mads Thomassen   +5 more
wiley   +1 more source

Lipolysis generates platelet dysfunction after in vivo heparin administration [PDF]

, 2002
Heparin, when administered to patients undergoing operations using cardiopulmonary bypass, induces plasma changes that gradually impair platelet macroaggregation, but heparinization of whole blood in vitro does not have this effect.
Belcher, P.R., Caslake, M.J., Chaudhry, M.A., Day, S., Muriithi, E.W., Wheatley, D.J.   +5 more
core   +1 more source

Establishment of a humanized patient‐derived xenograft mouse model of high‐grade serous ovarian cancer for preclinical evaluation of combination immunotherapy

Molecular Oncology, EarlyView.
We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric, Line Bjørge, Martine Rott Lode, Cecilie Fredvik Torkildsen, Pia Aehnlich, Rammah Elnour, Daniela Elena Costea, Lars Andreas Akslen, Liv Cecilie Vestrheim Thomsen, Emmet McCormack, Katrin Kleinmanns   +10 more
wiley   +1 more source

A clinically validated human capillary blood transcriptome test for global systems biology studies

BioTechniques, 2020
To prevent and treat chronic diseases, including cancer, a global application of systems biology is needed. We report here a whole blood transcriptome test that needs only 50 μl of capillary (fingerprick) blood.
Ryan Toma, Nathan Duval, Benjamin Pelle, Matthew M Parks, Vishakh Gopu, Pedro J Torres, Francine R Camacho, Nan Shen, Subha Krishnan, Andrew Hatch, Hal Tily, Ally Perlina, Guruduth Banavar, Momchilo Vuyisich   +13 more
doaj   +1 more source