Results 121 to 130 of about 3,076 (138)

Efforts to Install a Heterologous Wood-Ljungdahl Pathway in Clostridium acetobutylicum Enable the Identification of the Native Tetrahydrofolate (THF) Cycle and Result in Early Induction of Solvents

Metabolic Engineering, 2023
Here, we report the construction of a Clostridium acetobutylicum strain ATCC 824 (pCD07239) by heterologous expression of carbonyl branch genes (CD630_0723∼CD630_0729) from Clostridium difficile, aimed at installing a heterologous Wood-Ljungdahl pathway (WLP).
Eleftherios T Papoutsakis, Won Jun Kim, Sang Yup Lee   +2 more
exaly   +3 more sources

Metalloproteins/Metalloenzymes for the Synthesis of Acetyl-CoA in the Wood-Ljungdahl Pathway

Science in China Series B: Chemistry, 2009
This paper focuses on the group of metalloproteins/metalloenzymes in the acetyl-coenzyme A synthesis pathway of anaerobic microbes called Wood-Ljungdahl pathway, including formate dehydrogenase (FDH), corrinoid iron sulfur protein (CoFeSP), acetyl-CoA synthase (ACS) and CO dehydrogenase (CODH).
TAN Xiangshi
exaly   +2 more sources

The Role of Pyruvate Ferredoxin Oxidoreductase in Pyruvate Synthesis During Autotrophic Growth by the Wood-Ljungdahl Pathway [PDF]

Journal of Biological Chemistry, 2000
Pyruvate:ferredoxin oxidoreductase (PFOR) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA and CO(2). The catalytic proficiency of this enzyme for the reverse reaction, pyruvate synthase, is poorly understood. Conversion of acetyl-CoA to pyruvate links the Wood-Ljungdahl pathway of autotrophic CO(2) fixation to the reductive ...
Stephen W Ragsdale
exaly   +3 more sources

The Wood–Ljungdahl Pathway as a Key Component of Metabolic Versatility in Candidate Phylum Bipolaricaulota (Acetothermia, OP1)

Environmental Microbiology Reports, 2019
Summary The Wood–Ljungdahl (WL) pathway is an important component of the metabolic machinery in multiple anaerobic prokaryotes, including numerous yet‐uncultured bacterial phyla. The pathway can operate in the reductive and oxidative directions, enabling a wide range of metabolic processes.
Noha H Youssef, Mostafa S Elshahed, Ibrahim F Farag   +2 more
exaly   +3 more sources

Exergy-Based Performance Analysis of a Continuous Stirred Bioreactor for Ethanol and Acetate Fermentation From Syngas via Wood–Ljungdahl Pathway

Chemical Engineering Science, 2016
Abstract In this work, a thermodynamic framework was proposed to achieve improved process understanding of ethanol and acetate fermentation in a continuous stirred tank bioreactor from syngas through the Wood–Ljungdahl pathway. The bioreactor performance was evaluated using both conventional exergy and eco-exergy principles to identify the effect of ...
Meisam Tabatabaei, Mortaza Aghbashlo, Ghasem D Najafpour   +2 more
exaly   +2 more sources

Microbial production of methyl-uranium via the Wood-Ljungdahl pathway

Science of The Total Environment
The misuse of uranium is a major threat to human health and the environment. In microbial ecosystems, microbes deploy various strategies to cope with uranium-induced stress. However, the exact ecological strategies and mechanisms underlying uranium tolerance in microbes remain unclear.
Hangzheng, Zhao, Weifeng, Yue, Changming, Cao, Bo-Tao, Zhang, Ziyi, Zan, Guoxi, Lian, Fuxin, Zheng, Guangming, Xu, Junfeng, Dou   +8 more
openaire   +2 more sources

The Eastern and Western branches of the Wood/Ljungdahl pathway: how the East and West were won

BioFactors, 1997
Figure 1 is a transmission electron micrograph of Clostridium ljungdahlii [1]. It, like Acetobacterium woodii [2], was named after someone who made great contributions to the understanding of how anaerobes fix carbon dioxide. C. ljungdahlii was named after Lars Ljungdahl; A. woodii after Harland Wood. I had the good fortune to train under both Lars and
openaire   +2 more sources

Rapid Kinetic Studies of Acetyl-CoA Synthesis:  Evidence Supporting the Catalytic Intermediacy of a Paramagnetic NiFeC Species in the Autotrophic Wood−Ljungdahl Pathway

Biochemistry, 2002
CO dehydrogenase/acetyl-CoA synthase (CODH/ACS), a key enzyme in the Wood-Ljungdahl pathway of anaerobic CO(2) fixation, is a bifunctional enzyme containing CODH, which catalyzes the reversible two-electron oxidation of CO to CO(2), and ACS, which catalyzes acetyl-CoA synthesis from CoA, CO, and a methylated corrinoid iron-sulfur protein (CFeSP).
Javier, Seravalli, Manoj, Kumar, Stephen W, Ragsdale   +2 more
openaire   +2 more sources

Mechanism of Transfer of the Methyl Group from (6S)-Methyltetrahydrofolate to the Corrinoid/Iron−Sulfur Protein Catalyzed by the Methyltransferase from Clostridium thermoaceticum:  A Key Step in the Wood−Ljungdahl Pathway of Acetyl-CoA Synthesis

Biochemistry, 1999
The methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase (MeTr) from Clostridium thermoaceticum catalyzes transfer of the N5-methyl group from (6S)-methyltetrahydrofolate (CH3-H4folate) to the cobalt center of a corrinoid/iron-sulfur protein (CFeSP), forming methylcob(III)amide and H4folate.
J, Seravalli, S, Zhao, S W, Ragsdale
openaire   +2 more sources

Aromatase-independent estrogenesis: Wood-Ljungdahl pathway likely contributed to the emergence of estrogens in the biosphere

Abstract Androgen and estrogen, key sex hormones, were long thought to be exclusively produced by vertebrates. The O 2 -dependent aromatase that converts androgen to estrogen (estrogenesis) has never been identified in any prokaryotes.
Po-Hsiang Wang, Tien-Yu Wu, Yi-Lung Chen, Ronnie G. Gicana, Tzong-Huei Lee, Mei-Jou Chen, Tsun-Hsien Hsiao, Mei-Yeh Jade Lu, Yi-Li Lai, Tzi-Yuan Wang, Jeng-Yi Li, Yin-Ru Chiang   +11 more
openaire   +1 more source