Results 101 to 110 of about 19,615,720 (298)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Executive dysfunction in cerebral small vessel disease: A subdomain analysis with conventional and experimental assessment methods

Cerebral Circulation - Cognition and Behavior
Introduction: Executive functions and processing speed have been regarded as core cognitive domains affected in cerebral small vessel disease (SVD). Executive functions, as an umbrella concept, consist of a variety of different subcomponents, yet in ...
Hanna Jokinen, Hanna M. Laakso, Anne Arola, Teemu Paajanen, Jussi Virkkala, Teppo Särkämö, Tommi Makkonen, Heidi Heinonen, Johanna Pitkänen, Antti Korvenoja, Susanna Melkas   +10 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Heart Rate Variability in Sciatica Patients Referred to Spine Surgery

Global Spine Journal, 2012
Introduction There is some data that pain patients may have impaired autonomic nervous system function assessed by heart rate variability (HRV). However, only few studies have evaluated the effect of low back disorders on HRV. In this study, we evaluated
J. Södervall, J. Karppinen, J. Puolitaival, J. Siltala, A. Kiviniemi, M. Tulppo, A. Hautala   +6 more
doaj   +1 more source

Correlates of employment status in individuals with asthma: a cross-sectional survey

Journal of Occupational Medicine and Toxicology, 2017
Background This study aims to elucidate factors that among adults with asthma are associated with working full-time. Methods This cross-sectional survey of 2613 working-age adults with asthma included questions on asthma history, symptoms and use of ...
Saara Taponen, Lauri Lehtimäki, Kirsi Karvala, Ritva Luukkonen, Jukka Uitti   +4 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Effort Reward Imbalance and Work Ability among nurses and call-center operators

, 2011
The aim of the study is to investigate the relationship between effort/reward imbalance and work ability in the nursing and call-center sectors. A selfreported questionnaire was completed by 1,113 nurses and 1,106 call-center operators.
G. Castellini, P. Campanini, S. Punzi, G. Costa, D. Camerino, P.M. Conway   +5 more
core   +1 more source

Work Ability Index of Nurses in Different Departments of the Hospital [PDF]

Muhandisī-i bihdāsht-i ḥirfah/ī
Background and Objective: Due to the nature of shift work, nurses are under high stress, which can ultimately affect their work ability (WA). Considering the importance of the nursing profession, in this study, the work ability index (WAI) of nurses was ...
mahdieh rastimehr, adel mazloomi, Zeinab Kazemi   +2 more
doaj