Results 21 to 30 of about 437,411 (211)

Benchmarking cryo-EM Single Particle Analysis Workflow

Frontiers in Molecular Biosciences, 2018
Cryo electron microscopy facilities running multiple instruments and serving users with varying skill levels need a robust and reliable method for benchmarking both the hardware and software components of their single particle analysis workflow.
Laura Y. Kim, William J. Rice, Edward T. Eng, Mykhailo Kopylov, Anchi Cheng, Ashleigh M. Raczkowski, Kelsey D. Jordan, Daija Bobe, Clinton S. Potter, Bridget Carragher   +9 more
doaj   +1 more source

Workflow Analysis and Interface Design for 3D Gaussian Splatting Using a Hierarchical Task Analysis Approach

Applied Sciences
This study investigates the impact of user workflows and iterative decision-making on task efficiency in 3D Gaussian splatting (3DGS)-based 3D reconstruction. In current 3DGS workflows, the absence of clearly defined stages and structured processes leads
Hyoeun Choi, Heewon Kang, Hyunsuk Kim
doaj   +1 more source

Comparative Drug Response Profiling in Neuroblastoma Cell Lines and Patient‐Derived Tumor Organoids

Pediatric Blood &Cancer, EarlyView.
ABSTRACT High‐risk neuroblastoma remains a leading cause of pediatric cancer mortality, and improved preclinical models are needed to guide therapeutic developments. We screened seven high‐risk neuroblastoma cell lines and three patient‐derived tumor organoids with 528 compounds alongside bone marrow controls, and compared them with external datasets ...
Krzysztof Wierbiłowicz, Se Whee Sammy Park, Aironas Los, Ernest Liu, Nona Struyf, Tom Erkers, Sören Lehmann, Jan J. Molenaar, Jakob Stenman, Olli Kallioniemi, Päivi Östling, Brinton Seashore‐Ludlow, Kasper Karlsson   +12 more
wiley   +1 more source

“We Cannot Talk as a Family”: Complexities of Survivorship Communication Among Hispanic/Latino Young Adult Survivors of Childhood Cancer, Parents, and Clinicians

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Young adult childhood cancer survivors (YA‐CCS) have an increased risk of treatment‐related health complications throughout their lives. Lifelong survivorship care is recommended, yet many YA‐CCS experience disruptions in care during pediatric‐to‐adult transitions and do not receive recommended survivorship care.
Kevin Shanchen Pu, Nicole Fernández‐Viña, Briana C. Martin‐Villa, Daniela Ramirez, Rebecca M. Lewinsohn, Mary Smith, Heidi Boynton, Lidia Schapira, Stephanie M. Smith   +8 more
wiley   +1 more source

Measuring Workflow Interruptions in Endoscopy: Development and Interrater Reliability of a Structured Observation Tool

Endoscopy International Open
Background Workflow interruptions are common in procedural medicine and may increase cognitive load, stress, and the risk of errors. While interruptions and flow disruptions have been studied in surgical settings, little is known about ...
Christian Torres Reyes, Antonia Zörb, Elena Theiß, Nelina Dimitrova, Peter Ewald, Oscar Cahyadi, Alanna Ebigbo   +6 more
doaj   +1 more source

A Fast and Cost-Effective Genotyping Method for CRISPR-Cas9-Generated Mutant Rice Lines

Plants, 2023
With increasing throughput in both the generation and phenotyping of mutant lines in plants, it is important to have an efficient and reliable genotyping method.
Abdugaffor Ablazov, Abrar Felemban, Justine Braguy, Hendrik N. J. Kuijer, Salim Al-Babili   +4 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Tray Application Versus the Standard Surgical Procedure: A Prospective Evaluation

Surgeries
(1) Background: trays are surgery-specific sets of required materials and medical devices, assembled in consultation between manufacturer and user, and provided in a sterile package.
Dimitri Barski, Wilfried von Eiff, Jochen Cramer, Stefan Welter, Thomas Otto   +4 more
doaj   +1 more source

Tidyproteomics: an open-source R package and data object for quantitative proteomics post analysis and visualization

BMC Bioinformatics, 2023
Background The analysis of mass spectrometry-based quantitative proteomics data can be challenging given the variety of established analysis platforms, the differences in reporting formats, and a general lack of approachable standardized post-processing ...
Jeff Jones, Elliot J. MacKrell, Ting-Yu Wang, Brett Lomenick, Michael L. Roukes, Tsui-Fen Chou   +5 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source