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Transgenic Wucai (Brassica campestris L.) produced via Agrobacterium-mediated anther transformation in planta

Plant Cell Reports, 2019
We developed a novel Agrobacterium-mediated anther transformation for Wucai in planta, and in this procedure, the male germ line was the predominant target. Wucai (Brassica campestris L.), a variant of non-heading Chinese cabbage, is widely cultured in China and only improved by classic breeding methods.
Guohu Chen   +7 more
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Characterization and utilization of a cytoplasmic male sterility line of Wucai (Brassica campestris L.)

Horticulture, Environment, and Biotechnology, 2019
The novel male sterility line 12-14A of Wucai (Brassica campestris) was generated by backcrossing. In this study, morphological, molecular, and cytological analyses were performed to characterize this line. The phenotypes of flower organs of 12-14A were different from those of its maintainer line 12-14B, as the flowers of 12-14A were completely ...
Guohu Chen   +8 more
openaire   +1 more source

TMT-label comparative proteomics reveals the vernalization mechanism in Wucai (Brassica campestris L.)

Journal of Proteomics
To investigate the molecular basis of vernalization in Wucai [Brassica campestris L. (Syn. Brassica rapa L.) ssp. chinensis var. rosularis Tsen], we performed differential proteomic analysis using a tandem mass tags (TMT)-based approach. Proteins from shoot apices subjected to 0, 15, and 30 days of vernalization (V0, V15, and V30) were analyzed to ...
Xueqing, Liu   +8 more
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Functional analysis of a MYB transcription factor BrTDF1 in the tapetum development of Wucai (Brassica rapa ssp.)

Scientia Horticulturae, 2019
Abstract Ogura cytoplasmic male sterility (ogu-CMS) has been widely used by breeders for vegetables in the genus Brassica; however, the molecular mechanism of this male sterility remains unclear. The tapetum plays a crucial role in anther development by providing nutrients and secreting callose for pollen development. In this study, we found that the
Shengyun Zhang   +8 more
openaire   +1 more source

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