Results 71 to 80 of about 9,583,295 (252)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Some Insights into Mechanisms Involved in Continuous Casting

High Temperature Materials and Processes, 2012
A mathematical model of the continuous casting process for steel has been developed. This coupled model predicts metal flow, slag infiltration, shell solidification and heat flux. The model predicts values for the thicknesses of the solid and liquid slag
Mills K. C., Ramirez-Lopez P. E., Lee P. D.   +2 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

The collapse of a sonoluminescent cavitation bubble imaged with X-ray free-electron laser pulses

New Journal of Physics
Single bubble sonoluminescence (SBSL) is the phenomenon of synchronous light emission due to the violent collapse of a single spherical bubble in a liquid, driven by an ultrasonic field.
Hannes P Hoeppe, Markus Osterhoff, Atiyeh Aghel Maleki, Juan M Rosselló, Malte Vassholz, Johannes Hagemann, Thea Engler, Daniel Schwarz, Angel Rodriguez-Fernandez, Ulrike Boesenberg, Johannes Möller, Roman Shayduk, Jörg Hallmann, Anders Madsen, Robert Mettin, Tim Salditt   +15 more
doaj   +1 more source

Automatic unhulled rice grain crack detection by X-ray imaging [PDF]

, 2007
Crack detection of incoming paddy (unhulled rice grain) is an important step in the rice milling industry, as paddy grains with cracks severely affect the milling yield.
Bal, Satish, Pothula, Anand Kumar
core  

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

X-ray phase-contrast computed tomography for full-breast mastectomy imaging at the Australian Synchrotron

, 2021
One of the imaging modalities offered by the Imaging and Medical Beamline (IMBL) at the Australian Synchrotron is Xray phase-contrast propagation-based computed tomography (PB-CT). The unique combination of high coherence and high brightness of radiation
Stevenson, Andrew W., Dimmock, Matthew, Hasermann, Daniel, Maksimenko, Anton, Lewis, Sarah (R21243), Nesterets, Yakov I., Taba, Seyedamir T., Quiney, Harry M., Hall, Christopher J., Thompson, Darren, Mayo, Sheridan C., Arhatari, Benedicta D., Brennan , Patrick C., Gureyev, Timur E., Patrick C. Brennan, Darren Thompson, Sheridan C. Mayo, Seyedamir T. Taba, Timur E. Gureyev, Christopher J. Hall, Harry M. Quiney, Daniel Hausermann, Sarah J. Lewis, Anton Maksimenko, Benedicta D. Arhatari, Yakov I. Nesterets, Andrew W. Stevenson, Matthew Dimmock   +27 more
core   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source