Results 161 to 170 of about 15,789 (195)

Cryopreservation of sperm ofXenopus laevis andXenopus tropicalis

Genesis, 2005
Now that transgenic strains of Xenopus laevis and X. tropicalis can be generated efficiently and with genomic sequence resources available for X. tropicalis, early amphibian development can be studied using integrated biochemical and genetic approaches.
Michael G Sargent
exaly   +3 more sources

Husbandry of Xenopus tropicalis

2012
Xenopus tropicalis combine the advantages of X. laevis, for example using explants and targeted gain of function, with the ability to take classical genetics approaches to answering cell and developmental biology questions making it arguably the most versatile of the model organisms. Against this background, husbandry of X.
Jafkins, Alan, Abu-Daya, Anita, Noble, Anna, Zimmerman, Lyle B., Guille, Matt   +4 more
openaire   +3 more sources

Construction of BAC library for the amphibian Xenopus tropicalis

Genes and Genetic Systems, 2004
Xenopus tropicalis has become an alternative model to the amphibian Xenopus laevis because it is better suited for genetic and genomic studies. We have constructed a genomic BAC library consisting of over 100,000 clones from sperm of Xenopus tropicalis. Analysis by pulsed field gel electrophoresis of representative BAC clones indicated the average size
Shoko Ishibashi, Takeshi Yagi
exaly   +4 more sources

Developmental Genetics in Xenopus tropicalis

2011
The diploid pipid frog Xenopus tropicalis has recently emerged as a powerful new model system for combining genetic and genomic analysis of tetrapod development with embryological and biochemical assays. Its early development closely resembles that of its well-understood tetraploid relative Xenopus laevis, from which techniques and reagents can be ...
Timothy J, Geach, Lyle B, Zimmerman
openaire   +2 more sources

Best Practices forXenopus tropicalisHusbandry

Cold Spring Harbor Protocols, 2022
Xenopus tropicalishas been adopted by laboratories as a developmental genetic system because of its diploid genome and short generation time, contrasting withXenopus laevis, which is allotetraploid and takes longer to reach sexual maturity. BecauseX.
Takuya Nakayama, Robert M. Grainger
openaire   +2 more sources

Xenopus tropicalis Oocytes

2006
For more than 30 yr, Xenopus laevis has been the animal of choice for studying the biochemical regulation of the meiotic and early mitotic vertebrate cell cycles. Attracted by its diploid genome, several laboratories have begun using the similar, although evolutionarily distinct, frog Xenopus tropicalis for studies of vertebrate development ...
Jean-François L. Bodart, Nicholas S. Duesbery   +1 more
openaire   +1 more source

Navigating the Xenopus tropicalis Genome

2012
The frog Xenopus laevis has for more than 60 years served as a model system for the study of vertebrate embryogenesis, molecular and cell biology, and physiology. Recently, there has been great interest in the related species Xenopus tropicalis, in part because it is diploid, unlike the allotetraploid X.
openaire   +2 more sources

Teratogenic effects of tetrabromobisphenol A on Xenopus tropicalis embryos

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2010
Tetrabromobisphenol A (TBBPA) is the most widely used brominated flame retardant and a known thyroid disruptor. We reported exposing Xenopus tropicalis embryos (NF10) to 0.01, 0.1 or 1 mg/L of TBBPA with or without 70 microg/L triiodothyronine (T(3)). Compared with the controls, 1 mg/L of TBBPA significantly reduced the body length of embryos after 24,
Huahong, Shi, Lijuan, Qian, Suzhen, Guo, Xiaoli, Zhang, Junqi, Liu, Qinzhen, Cao   +5 more
openaire   +2 more sources

Genetic and genomic prospects for Xenopus tropicalis research

Seminars in Cell & Developmental Biology, 2006
Research using Xenopus laevis has made enormous contributions to our understanding of vertebrate development, control of the eukaryotic cell cycle and the cytoskeleton. One limitation, however, has been the lack of systematic genetic studies in Xenopus to complement molecular and cell biological investigations.
Samantha, Carruthers, Derek L, Stemple
openaire   +2 more sources

Cornea-lens transdifferentiation in the anuran, Xenopus tropicalis

Development Genes and Evolution, 2001
Previously, the only anuran amphibian known to regenerate the lens of the eye was Xenopus laevis. This occurs during larval stages through transdifferentiation of the outer cornea epithelium under control of factors presumably secreted by the neural retina. This study demonstrates that a distantly related species, X.
J J, Henry, M B, Elkins
openaire   +2 more sources