Results 161 to 170 of about 15,671 (201)
Some of the next articles are maybe not open access.
Microinjection of Xenopus tropicalis Embryos
Cold Spring Harbor Protocols, 2021Microinjection is an important technique used to study development in the oocyte and early embryo. In Xenopus, substances such as DNA, mRNA, and morpholino oligonucleotides have traditionally been injected into Xenopus laevis, because of their large embryo size and the relatively long time from their fertilization to first division.
Maura Lane +2 more
openaire +2 more sources
Best Practices forXenopus tropicalisHusbandry
Cold Spring Harbor Protocols, 2022Xenopus tropicalishas been adopted by laboratories as a developmental genetic system because of its diploid genome and short generation time, contrasting withXenopus laevis, which is allotetraploid and takes longer to reach sexual maturity. BecauseX.
Takuya Nakayama, Robert M. Grainger
openaire +2 more sources
Husbandry of Xenopus tropicalis
2012Xenopus tropicalis combine the advantages of X. laevis, for example using explants and targeted gain of function, with the ability to take classical genetics approaches to answering cell and developmental biology questions making it arguably the most versatile of the model organisms. Against this background, husbandry of X.
Jafkins, Alan +4 more
openaire +3 more sources
Developmental Genetics in Xenopus tropicalis
2011The diploid pipid frog Xenopus tropicalis has recently emerged as a powerful new model system for combining genetic and genomic analysis of tetrapod development with embryological and biochemical assays. Its early development closely resembles that of its well-understood tetraploid relative Xenopus laevis, from which techniques and reagents can be ...
Timothy J, Geach, Lyle B, Zimmerman
openaire +2 more sources
2006
For more than 30 yr, Xenopus laevis has been the animal of choice for studying the biochemical regulation of the meiotic and early mitotic vertebrate cell cycles. Attracted by its diploid genome, several laboratories have begun using the similar, although evolutionarily distinct, frog Xenopus tropicalis for studies of vertebrate development ...
Jean-François L. Bodart +1 more
openaire +1 more source
For more than 30 yr, Xenopus laevis has been the animal of choice for studying the biochemical regulation of the meiotic and early mitotic vertebrate cell cycles. Attracted by its diploid genome, several laboratories have begun using the similar, although evolutionarily distinct, frog Xenopus tropicalis for studies of vertebrate development ...
Jean-François L. Bodart +1 more
openaire +1 more source
Obtaining Xenopus tropicalis Embryos by Natural Mating
Cold Spring Harbor Protocols, 2021Xenopus is a powerful model system for cell and developmental biology in part because frogs produce thousands of eggs and embryos year-round. Natural matings are a simple and common method to obtain embryos for injection or other experimental use or to raise to adulthood.
Maura Lane, Mustafa K. Khokha
openaire +2 more sources
Generating Diploid Embryos from Xenopus tropicalis
2012A spectacular advantage of Xenopus tropicalis is the ease with which diploid embryos can be generated year round. By the simple administration of human chorionic gonadotropin, an investigator can generate many hundreds of synchronized embryos by in vitro fertilization or thousands of embryos from a mating pair.
Florencia, del Viso, Mustafa, Khokha
openaire +2 more sources
Xenopus tropicalis (Gray, 1864) (Fig. 12C) MATERIAL EXAMINED. — Togo • 4 ♀; Agoté; MNHN-RA-2006.2172-2175. DESCRIPTION. — Medium to large frog (SVL 40-56 mm ♀), with flattened body. Snout rounded. Head very small, wider than long (HW 26-29% SVL; HL 21-26% SVL). Very small eyes and eyelids (EL 1.4-1.6 mm diameter); a very small subocular tentacle, about
Segniagbeto, Gabriel Hoinsoudé +4 more
openaire +1 more source
Segniagbeto, Gabriel Hoinsoudé +4 more
openaire +1 more source
Navigating the Xenopus tropicalis Genome
2012The frog Xenopus laevis has for more than 60 years served as a model system for the study of vertebrate embryogenesis, molecular and cell biology, and physiology. Recently, there has been great interest in the related species Xenopus tropicalis, in part because it is diploid, unlike the allotetraploid X.
openaire +2 more sources
Cryopreservation of sperm ofXenopus laevis andXenopus tropicalis
genesis, 2005Now that transgenic strains of Xenopus laevis and X. tropicalis can be generated efficiently and with genomic sequence resources available for X. tropicalis, early amphibian development can be studied using integrated biochemical and genetic approaches.
Michael G, Sargent, Timothy J, Mohun
openaire +2 more sources