Results 151 to 160 of about 10,041 (187)
Comparative Distribution of Repetitive Sequences in the Karyotypes of Xenopus tropicalis and Xenopus laevis (Anura, Pipidae) [PDF]
Genes, 2021 Xenopus laevis and its diploid relative, Xenopus tropicalis, are the most used amphibian models. Their genomes have been sequenced, and they are emerging as model organisms for research into disease mechanisms.
Alvaro S Roco +2 more
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Molecular components of the endoderm specification pathway inXenopus tropicalis [PDF]
Developmental Dynamics, 2003 Xenopus laevis has been instrumental in elucidating a conserved molecular pathway that regulates vertebrate endoderm specification. However, loss-of-function analysis is required to resolve the precise function of the genes involved.
Nancy Papalopulu, Jim Smith
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Xenopus tropicalis: An Ideal Experimental Animal in Amphibia
Experimental Animals, 2010 Studies using amphibians have contributed to the progress of life science including developmental biology and cell biology for more than one hundred years. Since the 1950s Xenopus laevis in particular has been used by scientists in many fields for experiments, resulting in the development of various techniques such as microsurgery on early embryos ...
Keisuke Nakajima +2 more
exaly +4 more sources
Cloning and expression of the Cdx family from the frogXenopus tropicalis [PDF]
Developmental Dynamics, 2002 The caudal-related (Cdx) homeodomain transcription factors have a conserved role in the development of posterior structures in both vertebrates and invertebrates.
Iain D Keenan
exaly +2 more sources
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Obtaining Xenopus tropicalis Eggs
Cold Spring Harbor Protocols, 2021Xenopus is a powerful model system for cell and developmental biology in part because frogs produce thousands of eggs and embryos year-round. For cell biological studies, egg extracts can mimic many processes in a cell-free system. For developmental biology, Xenopus embryos are a premier system, combining cut-and-paste embryology with modern gene ...
Maura Lane +2 more
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Microinjection of Xenopus tropicalis Embryos
Cold Spring Harbor Protocols, 2021Microinjection is an important technique used to study development in the oocyte and early embryo. In Xenopus, substances such as DNA, mRNA, and morpholino oligonucleotides have traditionally been injected into Xenopus laevis, because of their large embryo size and the relatively long time from their fertilization to first division.
Maura Lane +2 more
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Husbandry of Xenopus tropicalis
2012Xenopus tropicalis combine the advantages of X. laevis, for example using explants and targeted gain of function, with the ability to take classical genetics approaches to answering cell and developmental biology questions making it arguably the most versatile of the model organisms. Against this background, husbandry of X.
Jafkins, Alan +4 more
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Construction of BAC library for the amphibian Xenopus tropicalis
Genes and Genetic Systems, 2004 Xenopus tropicalis has become an alternative model to the amphibian Xenopus laevis because it is better suited for genetic and genomic studies. We have constructed a genomic BAC library consisting of over 100,000 clones from sperm of Xenopus tropicalis. Analysis by pulsed field gel electrophoresis of representative BAC clones indicated the average size
Takeshi Yagi, Shoko Ishibashi
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Developmental Genetics in Xenopus tropicalis
2011The diploid pipid frog Xenopus tropicalis has recently emerged as a powerful new model system for combining genetic and genomic analysis of tetrapod development with embryological and biochemical assays. Its early development closely resembles that of its well-understood tetraploid relative Xenopus laevis, from which techniques and reagents can be ...
Timothy J, Geach, Lyle B, Zimmerman
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Best Practices forXenopus tropicalisHusbandry
Cold Spring Harbor Protocols, 2022Xenopus tropicalishas been adopted by laboratories as a developmental genetic system because of its diploid genome and short generation time, contrasting withXenopus laevis, which is allotetraploid and takes longer to reach sexual maturity. BecauseX.
Takuya Nakayama, Robert M. Grainger
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