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Preparation of Xenopus borealis and Xenopus tropicalis Egg Extracts for Comparative Cell Biology and Evolutionary Studies. [PDF]
Methods Mol BiolKitaoka M +3 more
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Techniques and probes for the study of Xenopus tropicalis development [PDF]
Developmental Dynamics, 2002 The frog Xenopus laevis has provided significant insights into developmental and cellular processes. However, X. laevis has an allotetraploid genome precluding its use in forward genetic analysis.
Nancy Papalopulu +2 more
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Comparative Distribution of Repetitive Sequences in the Karyotypes of Xenopus tropicalis and Xenopus laevis (Anura, Pipidae) [PDF]
Genes, 2021 Xenopus laevis and its diploid relative, Xenopus tropicalis, are the most used amphibian models. Their genomes have been sequenced, and they are emerging as model organisms for research into disease mechanisms.
Alvaro S Roco +2 more
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Cloning and expression of the Cdx family from the frog Xenopus tropicalis
Developmental Dynamics, 2002 The caudal-related (Cdx) homeodomain transcription factors have a conserved role in the development of posterior structures in both vertebrates and invertebrates.
Harry Victor Isaacs, Iain D Keenan
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Microinjection of Xenopus tropicalis Embryos
Cold Spring Harbor Protocols, 2021Microinjection is an important technique used to study development in the oocyte and early embryo. In Xenopus, substances such as DNA, mRNA, and morpholino oligonucleotides have traditionally been injected into Xenopus laevis, because of their large embryo size and the relatively long time from their fertilization to first division.
Maura Lane +2 more
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Molecular components of the endoderm specification pathway in Xenopus tropicalis
Developmental Dynamics, 2003 Xenopus laevis has been instrumental in elucidating a conserved molecular pathway that regulates vertebrate endoderm specification. However, loss-of-function analysis is required to resolve the precise function of the genes involved.
Nancy Papalopulu +2 more
exaly +2 more sources
Obtaining Xenopus tropicalis Eggs
Cold Spring Harbor Protocols, 2021Xenopus is a powerful model system for cell and developmental biology in part because frogs produce thousands of eggs and embryos year-round. For cell biological studies, egg extracts can mimic many processes in a cell-free system. For developmental biology, Xenopus embryos are a premier system, combining cut-and-paste embryology with modern gene ...
Maura Lane +2 more
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Xenopus tropicalis: An Ideal Experimental Animal in Amphibia
Experimental Animals, 2010 Studies using amphibians have contributed to the progress of life science including developmental biology and cell biology for more than one hundred years. Since the 1950s Xenopus laevis in particular has been used by scientists in many fields for experiments, resulting in the development of various techniques such as microsurgery on early embryos ...
Yoshio Yaoita +2 more
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Cryopreservation of sperm of Xenopus laevis and Xenopus tropicalis
Genesis, 2005Now that transgenic strains of Xenopus laevis and X. tropicalis can be generated efficiently and with genomic sequence resources available for X. tropicalis, early amphibian development can be studied using integrated biochemical and genetic approaches.
Timothy J Mohun
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Husbandry of Xenopus tropicalis
2012Xenopus tropicalis combine the advantages of X. laevis, for example using explants and targeted gain of function, with the ability to take classical genetics approaches to answering cell and developmental biology questions making it arguably the most versatile of the model organisms. Against this background, husbandry of X.
Jafkins, Alan +4 more
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