Results 171 to 180 of about 4,761 (203)
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Recent progress in the assays of xylanolytic enzymes
Journal of the Science of Food and Agriculture, 2006AbstractIncreasing industrial importance of xylanolytic enzymes together with recent evidence for cereal endoxylanase inhibitors having a negative impact on the application of enzymes in fodder and food industries, brings challenges to facilitate and simplify the procedures to follow these enzymes.
Peter Biely, Vladimír Puchart
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Extracellular xylanolytic enzymes ofPaecilomyces varioti
Biotechnology Letters, 1989Paecilomycesvarioti produced an extracellular xylanase and B-xylosidase when cultured in a medium containing xylan and corn steep liquor. Xylose (2%, w/v) totally inhibited production of both enzymes. The enzymes were purified and both had a pH optimum of 4.0.
C. T. Kelly +2 more
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Journal of Microbiological Methods, 2011
Miniaturized fungal cultivation and enzyme assays were developed. Cultivation for enzyme production was performed in 50mL conical tubes. In addition, the miniaturized enzyme assays reduced the amount of enzymes and reagents necessary. These procedures can be adopted in screening fungi to determine if they produce cellulolytic and xylanolytic enzymes.
Young Min, Lee +3 more
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Miniaturized fungal cultivation and enzyme assays were developed. Cultivation for enzyme production was performed in 50mL conical tubes. In addition, the miniaturized enzyme assays reduced the amount of enzymes and reagents necessary. These procedures can be adopted in screening fungi to determine if they produce cellulolytic and xylanolytic enzymes.
Young Min, Lee +3 more
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New methodology for fungal screening: Xylanolytic enzymes
Biotechnology Techniques, 1993The technique for determining extracellular xylanolytic activity consists of growing selected fungi for four days on agar medium containing 1% xylan. A section of the agar is then homogenized in buffer and the filtered solution tested for xylanase activity and other related enzymes.
E. Curotto +8 more
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Influence of growth conditions on the production of xylanolytic enzymes by Aspergillus flavus
Journal of Basic Microbiology, 1999Investigations were carried out to optimize the culture conditions for the production of xylanase and beta-xylosidase by Aspergillus flavus, a filamentous fungus isolated from soil. The production of enzymes was tolerant to a wide range of initial culture pH values.
C G, de Souza +3 more
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Detection and assay of xylanolytic enzymes in a Cellulomonas isolate
Biotechnology Letters, 1980The accuracy of xylanase assay was tested and improved. The assay was used to monitor xylanase production by a Cellulomonas isolate and to demonstrate that this activity is distinct from the organism's β-xylosidase activity.
P. A. D. Rickard, T. A. Laughlin
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Separation of the cellulytic and xylanolytic enzymes of Clostridium stercorarium
Journal of Chromatography A, 1990Abstract Exoenzymes of the thermophilic, anaerobic bacterium Clostridium stercorarium involved in cellulose and arabinoxylan degradation were resolved by a combination of fast protein liquid chromatographic (FPLC) chromatofocusing and FPLC anion-exchange chromatography.
K. Bronnenmeier +2 more
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Microbial Xylanolytic Enzyme System: Properties and Applications
1997Publisher Summary The chapter introduces xylan as a major component of plant hemicellulose. It is the most abundant renewable polysaccharide in nature after cellulose. The chapter describes its structure and its interaction with plant cell walls.
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The Xylanolytic Enzyme System of Trichoderma reesei
1989The xylanolytic enzyme system of Trichoderma reesei, a well-known producer of cellulolytic enzymes, is versatile and well suited for the total hydrolysis of different xylans. It consists of two major, specific and several non-specific xylanases, at least one β-xylosidase, α-arabinosidase and α-glucuronidase and at least two acetyl esterases.
Kaisa Poutanen, Jurgen Puls
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