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, 2021 Lignocellulosic (LC) feedstock is the most economical and renewable natural feedstock of chemicals and energy. It is primarily composed of lignin, cellulose, and hemicellulose which are woven together and render the biomass recalcitrant to degradation.
Rozina Rashid +2 more
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Xylose absorption test using D-xylose-14C
Clinical Biochemistry, 1972Summary 1. D-xylose-14C was used to measure oral xylose absorption in the investigation of malabsorption syndromes. The concentration of D-xylose-14C present in the 1 and 2 hour serum samples and the quantity of D-xylose and D-xylose-14C excreted in the urine in 5 hours were used as indices of xylose absorption. 2.
E.K. Mincey +4 more
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Journal of Clinical Gastroenterology, 1999
The literature on D-xylose testing has been reviewed, stressing advances in our understanding of absorption in general (including D-xylose absorption), the relationship of D-xylose testing to the development of excellent serologic tests for the diagnosis of celiac disease, the use of D-xylose testing in the evaluation of diarrhea in acquired ...
Eli D. Ehrenpreis, Robert M. Craig
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The literature on D-xylose testing has been reviewed, stressing advances in our understanding of absorption in general (including D-xylose absorption), the relationship of D-xylose testing to the development of excellent serologic tests for the diagnosis of celiac disease, the use of D-xylose testing in the evaluation of diarrhea in acquired ...
Eli D. Ehrenpreis, Robert M. Craig
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Evolution in Reverse: Engineering a
ChemBioChem, 2008 Xylitol (1) is a pentitol and is used not only as a sweetener but also as a platform chemical for the production of industrially important chemicals. As a sweetener, it has been shown to possess several favorable properties in comparison to other sugar substitutes, such as anticariogenicity, good gastrointestinal tolerance, low caloric content, and ...
Huimin Zhao, Nikhil U. Nair
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Repression of xylose utilization by glucose in xylose-fermenting yeasts
Canadian Journal of Microbiology, 1988The xylose-fermenting yeasts Pichia stipitis, Candida steatolytica, and Candida shehatae were subjected to fermentations in synthetic media containing mixtures of glucose and xylose. In all cases, repression of xylose uptake by glucose was observed, although the extent of repression was different with each yeast.
Inge Russell +3 more
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Reduction of PDC1 expression in S. cerevisiae with xylose isomerase on xylose medium
Bioprocess and Biosystems Engineering, 2011Ethanol production using hemicelluloses has recently become a focus of many researchers. In order to promote D: -xylose fermentation, we cloned the bacterial xylA gene encoding for xylose isomerase with 434 amino acid residues from Agrobacterium tumefaciens, and successfully expressed it in Saccharomyces cerevisiae, a non-xylose assimilating yeast. The
Gwon-Young Jeong +9 more
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Determination of the anomeric configuration of D-xylose with D-xylose isomerases
Carbohydrate Research, 1976Abstract The anomeric configuration of D -xylose, resulting from hydrolysis of β- D xylopyranosides by β- D -xylosidase from Bacillus pumilus , has been determined by an enzymic procedure, based on the stereospecificity of D -xylose isomerases. The initial hydrolysis product is α- D -xylose.
Clement K. De Bruyne +3 more
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Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes
Biotechnology Letters, 2015To genetically engineer Saccharomyces cerevisiae for improved ethanol productivity from glucose/xylose mixtures.An endogenous gene cassette composed of aldose reductase (GRE3), sorbitol dehydrogenase (SOR1) and xylulose kinase (XKS1) with a PGK1 promoter and a terminator was introduced into two S.
Akira Fukuda +3 more
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Applied Microbiology and Biotechnology, 2008
The goal of this investigation was to determine the effect of a xylose transport system on glucose and xylose co-consumption as well as total xylose consumption in Saccharomyces cerevisiae. We expressed two heterologous transporters from Arabidopsis thaliana in recombinant xylose-utilizing S. cerevisiae cells.
Stephen R. Hughes +3 more
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The goal of this investigation was to determine the effect of a xylose transport system on glucose and xylose co-consumption as well as total xylose consumption in Saccharomyces cerevisiae. We expressed two heterologous transporters from Arabidopsis thaliana in recombinant xylose-utilizing S. cerevisiae cells.
Stephen R. Hughes +3 more
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Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae
Biotechnology and Bioengineering, 2003AbstractSaccharomyces cerevisiae TMB3001 has previously been engineered to utilize xylose by integrating the genes coding for xylose reductase (XR) and xylitol dehydrogenase (XDH) and overexpressing the native xylulokinase (XK) gene. The resulting strain is able to metabolize xylose, but its xylose utilization rate is low compared to that of natural ...
Gunnar Lidén +4 more
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