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Evolution in Reverse: Engineering a D‐Xylose‐Specific Xylose Reductase [PDF]

ChemBioChem, 2008
Xylitol (1) is a pentitol and is used not only as a sweetener but also as a platform chemical for the production of industrially important chemicals. As a sweetener, it has been shown to possess several favorable properties in comparison to other sugar substitutes, such as anticariogenicity, good gastrointestinal tolerance, low caloric content, and ...
Huimin Zhao, Nikhil U. Nair
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Repression of xylose utilization by glucose in xylose-fermenting yeasts

Canadian Journal of Microbiology, 1988
The xylose-fermenting yeasts Pichia stipitis, Candida steatolytica, and Candida shehatae were subjected to fermentations in synthetic media containing mixtures of glucose and xylose. In all cases, repression of xylose uptake by glucose was observed, although the extent of repression was different with each yeast.
Inge Russell, Chandra J. Panchal, Graham G. Stewart, Lynda Bast   +3 more
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Engineering prokaryotic transcriptional activator XylR as a xylose-inducible biosensor for transcription activation in yeast.

ACS Synthetic Biology, 2020
Biosensors regulated by specific substrates are needed to develop genetic tools to meet the needs of engineering microbial cell factories. Here, a xylose-inducible biosensor (Xylbiosensor), comprising the Escherichia coli activation factor XylR, fusion ...
Wenping Wei, Yanzhe Shang, Ping Zhang, Yong Liu, D. You, Bincheng Yin, Bangce Ye   +6 more
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Reduction of PDC1 expression in S. cerevisiae with xylose isomerase on xylose medium

Bioprocess and Biosystems Engineering, 2011
Ethanol production using hemicelluloses has recently become a focus of many researchers. In order to promote D: -xylose fermentation, we cloned the bacterial xylA gene encoding for xylose isomerase with 434 amino acid residues from Agrobacterium tumefaciens, and successfully expressed it in Saccharomyces cerevisiae, a non-xylose assimilating yeast. The
Gwon-Young Jeong, Kyung-Hwan Jeong, Byung Sam Ko, Seunghyun Choi, Jae-Gun Han, Il-Chul Kim, Yonggwan Won, Han-Bit Jang, Dongmin Kim, Hyeon-Yong Lee   +9 more
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Determination of the anomeric configuration of D-xylose with D-xylose isomerases

Carbohydrate Research, 1976
Abstract The anomeric configuration of D -xylose, resulting from hydrolysis of β- D xylopyranosides by β- D -xylosidase from Bacillus pumilus , has been determined by an enzymic procedure, based on the stereospecificity of D -xylose isomerases. The initial hydrolysis product is α- D -xylose.
Clement K. De Bruyne, Marc Claeyssens, Elisa Van Doorslaer, Hilda Kersters-Hilderson   +3 more
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Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes

Biotechnology Letters, 2015
To genetically engineer Saccharomyces cerevisiae for improved ethanol productivity from glucose/xylose mixtures.An endogenous gene cassette composed of aldose reductase (GRE3), sorbitol dehydrogenase (SOR1) and xylulose kinase (XKS1) with a PGK1 promoter and a terminator was introduced into two S.
Akira Fukuda, Kozue Mutaguchi, Takeshi Uemura, Jin Konishi   +3 more
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Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose improves aerobic xylose consumption

Applied Microbiology and Biotechnology, 2008
The goal of this investigation was to determine the effect of a xylose transport system on glucose and xylose co-consumption as well as total xylose consumption in Saccharomyces cerevisiae. We expressed two heterologous transporters from Arabidopsis thaliana in recombinant xylose-utilizing S. cerevisiae cells.
Stephen R. Hughes, Nasib Qureshi, Ronald E. Hector, Michael A. Cotta   +3 more
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Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Biotechnology and Bioengineering, 2003
AbstractSaccharomyces cerevisiae TMB3001 has previously been engineered to utilize xylose by integrating the genes coding for xylose reductase (XR) and xylitol dehydrogenase (XDH) and overexpressing the native xylulokinase (XK) gene. The resulting strain is able to metabolize xylose, but its xylose utilization rate is low compared to that of natural ...
Gunnar Lidén, Bärbel Hahn-Hägerdal, Marie Jeppsson, Márk Gárdonyi, Marie-Francoise Gorwa-Grauslund   +4 more
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Transport of xylose and glucose in the xylose-fermenting yeast Pichia stipitis

Applied Microbiology and Biotechnology, 1988
A low-affinity and a high-affinity sylose proton symport operated simultaneously in both starved and non-starved cells of Pichia stipitis. Glucose competed with xylose for transport by the low-affinity system and inhibited xylose transport by the high-affinity system non-competitively.
Stephanus G. Kilian, N. van Uden
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Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae

Journal of Biotechnology, 2012
Efficient conversion of xylose to ethanol is an essential factor for commercialization of lignocellulosic ethanol. To minimize production of xylitol, a major by-product in xylose metabolism and concomitantly improve ethanol production, Saccharomyces cerevisiae D452-2 was engineered to overexpress NADH-preferable xylose reductase mutant (XR(MUT)) and ...
Sung-Haeng Lee, Jin-Ho Seo, Tsutomu Kodaki, Yong-Cheol Park   +3 more
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