Results 181 to 190 of about 11,524 (221)

Thermoinactivation of β-xylosidase immobilized on nylon

1998
Abstract β-xylosidase was isolated and partially purified from Trichoderma reesei QM 9414 grown on wheat straw as the sole carbon source. The enzyme was attached covalently to nylon powder and thermoinactivation was carried out at temperatures between 55 and 75° C.
M.J. Dueñas, P. Estrada
openaire   +1 more source

Research Progress Concerning Fungal and Bacterial β-Xylosidases

Applied Biochemistry and Biotechnology, 2015
In the present review, we briefly summarize the biotechnological applications of microbial β-xylosidases in the processing of agro-industrial residues into fuels and chemicals and report the importance of using immobilization techniques to study the enzyme.
Adilson, Bosetto, Priscila Innocenti, Justo, Bruna, Zanardi, Simoni Spohr, Venzon, Luciana, Graciano, Elaine Luzia, dos Santos, Rita de Cássia Garcia, Simão   +6 more
openaire   +2 more sources

Biochemical properties of a beta-xylosidase from Clostridium cellulolyticum

Applied and Environmental Microbiology, 1995
A 43-kDa beta-xylosidase from Clostridium cellulolyticum was purified to homogeneity. The enzyme releases xylose from p-nitrophenylxylose and xylodextrins with a degree of polymerization ranging between 2 and 5. The N-terminal amino acid sequence of the enzyme showed homologies with three other bacterial beta-xylosidases.
S, Saxena, H P, Fierobe, C, Gaudin, F, Guerlesquin, J P, Belaich   +4 more
openaire   +2 more sources

Preparation and application of a fluorogenic substrate for endo-β-xylosidase

Journal of Biochemical and Biophysical Methods, 1989
The present paper describes a fluorometric assay for galactosaminoglycan-degrading endo-beta-xylosidase, utilizing glycosaminoglycan chains bearing a 4-methylumbelliferyl group at the reducing terminus as a substrate. This fluorogenic substrate is synthesized by human skin fibroblasts cultured in the presence of a fluorogenic xyloside, 4 ...
K, Takagaki, A, Kon, H, Kawasaki, T, Nakamura, M, Endo   +4 more
openaire   +2 more sources

Xylanases and β-xylosidase of Trichoderma liǵnorum

1988
Publisher Summary Trichoderma lignorum is a rich source of various extracellular polyand oligosaccharide-degrading enzymes—for example, xylanases, cellulase, amyloglucosidase, β-xylosidasey α-arabinosidase, and β-glucosidase. This chapter describes a simple procedure for the purification of two different xylanases, 1,4-β-D-xylan xylanohydrolase and a
Michael John, Jürgen Schmidt
openaire   +1 more source

Engineering lower inhibitor affinities in β-d-xylosidase

Applied Microbiology and Biotechnology, 2009
Beta-D-Xylosidase catalyzes hydrolysis of xylooligosaccharides to D-xylose residues. The enzyme, SXA from Selenomonas ruminantium, is the most active catalyst known for the reaction; however, its activity is inhibited by D-xylose and D-glucose (K (i) values of approximately 10(-2) M).
Zhanmin, Fan, Ling, Yuan, Douglas B, Jordan, Kurt, Wagschal, Chamroeun, Heng, Jay D, Braker   +5 more
openaire   +2 more sources

Glycoside Hydrolase Family 39 β-Xylosidases Exhibit β-1,2-Xylosidase Activity for Transformation of Notoginsenosides: A New EC Subsubclass

Journal of Agricultural and Food Chemistry, 2019
β-1,2-Xylosidase activity has not been recorded as an EC subsubclass. In this study, phylogenetic analysis and multiple sequence alignments revealed that characterized β-xylosidases of glycoside hydrolase family (GH) 39 were classified into the same subgroup with conserved amino acid residue positions participating in substrate recognition.
Rui Zhang, Na Li, Shujing Xu, Xiaowei Han, Chunyan Li, Xin Wei, Yu Liu, Tao Tu, Xianghua Tang, Junpei Zhou, Zunxi Huang   +10 more
openaire   +2 more sources

Coupling the pretreatment and hydrolysis of lignocellulosic biomass by the expression of beta‐xylosidases

Biotechnology and Bioengineering, 2017
AbstractThermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years.
Lucía Martín Pérez, Laura Benítez Casanova, Antonio J. Moreno Pérez, Dolores Pérez Gómez, Sandra Gavaldá Martín, Laura Ledesma‐García, Noelia Valbuena Crespo, Bruno Díez García, Francisco M. Reyes‐Sosa   +8 more
openaire   +3 more sources

Formation of esterase, xylanase and xylosidase by cellulolytic anaerobes

Biomass, 1987
Abstract Three mesophilic anaerobes, namely Acetivibrio cellulolyticus, Acetivibrio cellulosolvens and Bacteroides cellulosolvens and a thermophilic anaerobe, namely Clostridium thermocellum, produced xylanase, xylosidase and esterase activities when grown on medium containing cellulose.
A.W. Khan, K.A. Lamb, M.A. Forgie
openaire   +1 more source

The β-xylosidase production by yeastCryptococcus podzolicus

Russian Journal of Bioorganic Chemistry, 2000
In studying the β-xylosidase production by yeastCryptococcus podzolicus (Basidiomycetes), it was shown to be an inducible secretory enzyme. Xylooligosaccharides generated from xylan and methyl β-xyloside were found to induce the β-xylosidase synthesis.
openaire   +1 more source