Results 51 to 60 of about 5,459 (179)
Thioglycoligases have proved useful for bonding carbohydrates to non-sugar acceptors, however, the scope of these biocatalysts is usually limited. Here, the authors engineer a xylosidase into a thioglycoligase with the ability to form O-, N-, S- and Se ...
Manuel Nieto-Domínguez +11 more
doaj +1 more source
Integrated microbiological, genomic, and structural analyses of bacteria isolated from a Peruvian geothermal system reveal enzymatic potential for the transformation of polyesters such as PET. This study links experimental evidence with genomic determinants and in silico analyses that support the thermophilic bacteria biodegradation capacity.
Marco A. Rivera‐Jacinto +6 more
wiley +1 more source
Crystal structure of endo‐β‐1,6‐galactanase from Streptomyces avermitilis
The crystal structure of S. avermitilis endo‐β‐1,6‐galactanase belonging to GH30 subfamily 5 reveals the first structural framework for endo‐β‐1,6‐galactanase. The β‐1,6‐galactobiose‐bound complex identifies the catalytic subsites and a distal secondary sugar‐binding site, providing insight into β‐1,6‐galactan recognition.Endo‐β‐1,6‐galactanases ...
Zui Fujimoto +3 more
wiley +1 more source
The recombinant 40 kDa BoExXyl43A glycoside hydrolase family 43 (GH43) from bacterium Bacteroides ovatus exhibited highest specific activity (U/mg) against corn cob xylan (136.8), followed by Beechwood xylan (81.1), Carbosynth xylan (69.3), 4-O-D ...
Parmeshwar Vitthal Gavande +4 more
doaj +1 more source
ABSTRACT With the growing interest in functional foods and their role in promoting health beyond basic nutrition, increasing attention has been directed toward the modulation of gut microbiota as a key determinant of human well‐being. In this context, prebiotics and probiotics have emerged as central bioactive ingredients due to their capacity to ...
Paula Cavion Costa, Marli Camassola
wiley +1 more source
Two recombinant xylanases from marine bacteria, β‐1,3‐xylanase Xyl4 and mixed‐linkage xylanase AlXyn26A, were used to digest glucoxylan structures extracted from the green alga Caulerpa lentillifera and synthesized by automated glycan assembly. High‐performance liquid chromatography coupled with mass spectrometry (HPLC‐MS) analysis of the reaction ...
Nils H. Rustmeier +2 more
wiley +1 more source
In this study, we investigated the secretion mechanism of the hyper-secretion signal peptide-lacking β-xylosidase PtXyl43, a non-classically secreted protein, from the fungus Paecilomyces thermophila in Escherichia coli BL21(DE3).
Yalin Yang +8 more
doaj +1 more source
Abstract Excessive use of agrochemicals and frequent tillage have degraded vineyard soils, threatening their sustainability and grape quality. Cover crops and microbial inoculants are increasingly promoted as nature‐based strategies for vineyard regeneration, yet they are rarely evaluated together.
Raúl Ochoa‐Hueso +9 more
wiley +1 more source
A factorial rainfall × warming field experiment showed that drought and warming suppressed overall soil respiration but amplified rewetting‐induced CO2 pulses via contrasting effects of moisture, temperature, and substrate availability. Their interaction shifted seasonally, from additive (spring) to antagonistic (summer‐autumn) and synergistic (winter),
Pankaj Tiwari +9 more
wiley +1 more source
Thermostable ?-xylosidase from Thermomonospora curvata
produced a thermostable β-xylosidase during growth on birch xylan. The enzyme, extracted by sonication of early stationary phase mycelia, was purified by isoelectric focusing and size exclusion HPLC. The isoelectric point was pH 4.8. The molecular weight was estimated to be 102 000 by size exclusion HPLC and 112 000 by SDS-PAGE.
F Stutzenberger, A B Bodine
openaire +1 more source

