Results 161 to 170 of about 1,854 (190)

Biochemical properties of a beta-xylosidase from Clostridium cellulolyticum

Applied and Environmental Microbiology, 1995
A 43-kDa beta-xylosidase from Clostridium cellulolyticum was purified to homogeneity. The enzyme releases xylose from p-nitrophenylxylose and xylodextrins with a degree of polymerization ranging between 2 and 5. The N-terminal amino acid sequence of the enzyme showed homologies with three other bacterial beta-xylosidases.
S, Saxena, H P, Fierobe, C, Gaudin, F, Guerlesquin, J P, Belaich   +4 more
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Preparation and application of a fluorogenic substrate for endo-β-xylosidase

Journal of Biochemical and Biophysical Methods, 1989
The present paper describes a fluorometric assay for galactosaminoglycan-degrading endo-beta-xylosidase, utilizing glycosaminoglycan chains bearing a 4-methylumbelliferyl group at the reducing terminus as a substrate. This fluorogenic substrate is synthesized by human skin fibroblasts cultured in the presence of a fluorogenic xyloside, 4 ...
K, Takagaki, A, Kon, H, Kawasaki, T, Nakamura, M, Endo   +4 more
openaire   +2 more sources

Engineering lower inhibitor affinities in β-d-xylosidase

Applied Microbiology and Biotechnology, 2009
Beta-D-Xylosidase catalyzes hydrolysis of xylooligosaccharides to D-xylose residues. The enzyme, SXA from Selenomonas ruminantium, is the most active catalyst known for the reaction; however, its activity is inhibited by D-xylose and D-glucose (K (i) values of approximately 10(-2) M).
Zhanmin, Fan, Ling, Yuan, Douglas B, Jordan, Kurt, Wagschal, Chamroeun, Heng, Jay D, Braker   +5 more
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Coupling the pretreatment and hydrolysis of lignocellulosic biomass by the expression of beta‐xylosidases

Biotechnology and Bioengineering, 2017
AbstractThermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years.
Lucía Martín Pérez, Laura Benítez Casanova, Antonio J. Moreno Pérez, Dolores Pérez Gómez, Sandra Gavaldá Martín, Laura Ledesma‐García, Noelia Valbuena Crespo, Bruno Díez García, Francisco M. Reyes‐Sosa   +8 more
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Formation of esterase, xylanase and xylosidase by cellulolytic anaerobes

Biomass, 1987
Abstract Three mesophilic anaerobes, namely Acetivibrio cellulolyticus, Acetivibrio cellulosolvens and Bacteroides cellulosolvens and a thermophilic anaerobe, namely Clostridium thermocellum, produced xylanase, xylosidase and esterase activities when grown on medium containing cellulose.
A.W. Khan, K.A. Lamb, M.A. Forgie
openaire   +1 more source

The β-xylosidase production by yeastCryptococcus podzolicus

Russian Journal of Bioorganic Chemistry, 2000
In studying the β-xylosidase production by yeastCryptococcus podzolicus (Basidiomycetes), it was shown to be an inducible secretory enzyme. Xylooligosaccharides generated from xylan and methyl β-xyloside were found to induce the β-xylosidase synthesis.
openaire   +1 more source

Purification and characterization of ?-xylosidase from Aureobasidium pullulans

Applied Microbiology and Biotechnology, 1991
β-Xylosidase was obtained from Aureobasidium pullulans CBS 58475 with an activity of 0.35 units/ml culture filtrate. The production of the enzyme was strongly inducible. β-Xylosidase was purified in two steps by anion exchange and gel-permeation chromatography to high purity.
J. Dobberstein, C.C. Emeis
openaire   +1 more source

A β-glucosidase/xylosidase from the phytopathogenic oomycete, Phytophthora infestans

Phytochemistry, 2002
An 85-kDa beta-glucosidase/xylosidase (BGX1) was purified from the axenically grown phytopathogenic oomycete, Phytophthora infestans. The bgx1 gene encodes a predicted 61-kDa protein product which, upon removal of a 21 amino acid leader peptide, accumulates in the apoplastic space.
Brunner, F., Wirtz, W., Rose, J.K.C., Darvill, A.G., Govers, F., Scheel, D., Nürnberger, T.   +6 more
openaire   +2 more sources

A CASE OF β-XYLOSIDASE DEFICIENCY

The Lancet, 1970
C R, Payling-Wright, P R, Evans
openaire   +2 more sources

β-D-xylosidase in pig kidney

Biochimica et Biophysica Acta (BBA) - Enzymology, 1967
D, Robinson, H E, Abrahams
openaire   +2 more sources