Results 111 to 120 of about 613,719 (256)

A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Nature Communications, 2016
Plant synthetic biology offers the potential to re-engineer crops, but requires efficient methods to prepare constructs for transformation. Here Shih et al. develop jStack, a method that utilizes yeast homologous recombination and a library of DNA parts,
Patrick M. Shih, Khanh Vuu, Nasim Mansoori, Leïla Ayad, Katherine B. Louie, Benjamin P. Bowen, Trent R. Northen, Dominique Loqué   +7 more
doaj   +1 more source

Comparative Genomics and Heterologous Complementation Reveal Sequence and Structural Divergence of Selected Transcription Factors From Spathaspora passalidarum

Yeast, EarlyView.
Comparative genomic analyses identified substantial sequence and structural differences between transcription factors from Spathaspora passalidarum and their Saccharomyces cerevisiae counterparts. Heterologous expression of YAP1 and MSN‐like genes in corresponding S.
Fernanda Matias Albuini, Alex Gazolla de Castro, Lílian Emídio Ribeiro, Lizandra Cristina de Oliveira Figueiredo Gazolla, Carlos Augusto Rosa, Tiago Antônio de Oliveira Mendes, Luciano Gomes Fietto   +6 more
wiley   +1 more source

Splitting, joining and cutting : mechanistic studies of enzymes that manipulate DNA [PDF]

, 2010
Electronic version does not contain associated previously published materialDNA is a reactive and dynamic molecule that is continually damaged by both exogenous and endogenous agents.
McRobbie, Anne-Marie M.
core   +2 more sources

Stress activated protein kinase pathway modulates homologous recombination in fission yeast.

PLoS ONE, 2012
Rad52 is a key player in homologous recombination (HR), a DNA repair pathway that is dedicated to double strand breaks repair and recovery of perturbed replication forks.
Angela Bellini, Pierre-Marie Girard, Sarah Lambert, Ludovic Tessier, Evelyne Sage, Stefania Francesconi   +5 more
doaj   +1 more source

Mapping Allergen B‐ and T‐Cell Epitopes: Technological Advances and Their Role in Precision Allergy Therapy

Allergy, EarlyView.
ABSTRACT Allergic diseases arise from aberrant immune recognition of otherwise harmless environmental proteins and are driven by epitope‐specific interactions between allergens and the adaptive immune system. Although component‐resolved diagnostics have improved molecular characterisation of sensitization, they remain limited to whole‐allergen ...
Mark Møiniche, Josefine Corneliussen, Kristoffer H. Johansen, Alexandre Ruiz‐Carrasco, Caroline Paulsen, Yuchen Li, Carolina Barra, Sandhya Bangaru, Monica L. Fernández‐Quintero, Ewa Bartko, Lars Blom, Esperanza Rivera‐de‐Torre   +11 more
wiley   +1 more source

Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells [PDF]

, 2005
Class switch recombination (CSR) is a region-specific, transcriptionally regulated, nonhomologous recombinational process that is initiated by activation-induced cytidine deaminase (AID).
Lennart Hammarström, Hammarström, L,, Lahdesmaki, A, Aleksi Lähdesmäki, Wei Zhou, Andrew R. Gennery, Jones, AM, Michael R. Ehrenstein, Pan-Hammarstrom, Q, Hammarstrom, L, Zhou, W, Gennery, AR, Ehrenstein, MR, Anne-Marie Jones, Richard A. Gatti, Gatti, RA, Qiang Pan-Hammarström   +16 more
core   +1 more source

Evidence That the Protein Phosphatase Activity of PTEN Contributes to Embryonic Development and Tumor Suppression

Cancer Science, EarlyView.
Mice expressing only mutant PTEN Y138L, a protein which shows normal suppression of cellular AKT yet lacks protein phosphatase activity, die in utero, and heterozygous mice display a range of tumors. This indicates both the lipid and protein phosphatase activities of PTEN work together for normal tumor suppression and embryonic development.
Priyanka Tibarewal, Laura Spinelli, Nisha Kriplani, Helen Wise, Nadege Poncet, Giulia Marzano, Karen E. Anderson, Katarzyna M. Grzes, Zofia Varyova, Mahreen Adil, C. Peter Downes, Phillip T. Hawkins, Len R. Stephens, Kate G. Storey, Doreen A. Cantrell, Bart Vanhaesebroeck, Nicholas R. Leslie   +16 more
wiley   +1 more source

Lego DNA assembling, a simple in vitro method for constructing DNA molecule [PDF]

, 2010
Digestion-ligation based and recombination based methods for constructing recombinant DNA are the basic techniques in molecular biology and thus built one of the foundations for the modern life sciences. Here we describe a new strategy that can radically
Cong Wang, Haibo Zhang, Sizheng Su, Mo Xian, Huaiwei Liu, Xinglin Jiang, Jianming Yang, Yanning Zheng   +7 more
core  

Molecular mechanisms underlying the control of antigenic variation in African trypanosomes [PDF]

, 2010
African trypanosomes escape the host adaptive immune response by switching their dense protective coat of Variant Surface Glycoprotein (VSG). Each cell expresses only one VSG gene at a time from a telomeric expression site (ES).
Horn, David, McCulloch, Richard, Horn, David; id_orcid, David Horn, Richard McCulloch   +4 more
core   +1 more source

Genetic dissection reveals distinct contributions of the eS31 N‐terminal domain to translational accuracy in Saccharomyces cerevisiae

The FEBS Journal, EarlyView.
The eukaryote‐specific N‐terminal domain (NTD) of eS31 uses two distinct strategies to maintain translation fidelity. During elongation, a positively charged “hotspot” fine‐tunes the selection of incoming aa‐tRNA. During termination, the entire NTD acts as a structural scaffold to ensure the correct positioning of the release factor eRF1.
Qingxuan Gao, Hiroshi Otsuka, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source