Results 71 to 80 of about 613,719 (256)

Genetics of DNA replication and homologous recombination in arabidopsis [PDF]

, 2005
The integrity of genetic information of each cell is constantly subjected to various threats, originating from environmental and endogenous sources.
Schürmann, David
core   +1 more source

Streptococcal Mannose Phosphotransferase System Component IID Is a Novel RANK‐Binding Osteoclastogenic Factor

Advanced Science, EarlyView.
Streptococcal mannose phosphotransferase system component IID (Man‐PTSIID) is identified as a novel RANK‐binding osteoclastogenic factor. By directly binding to RANK and activating NF‐κB independently of TLR2, Man‐PTSIID drives osteoclastogenesis and inflammatory bone destruction, uncovering an unexpected microbial mechanism underlying streptococcal ...
Chaeyeon Park, Yeongkag Kwon, Yeonjin Lim, Jeong Woo Park, Hyun Jung Ji, Dongwook Lee, Jueun Lee, Sung‐Ho Yun, Sungho Jeong, Ho Seong Seo, Kun Cho, Cheol‐Heui Yun, Ok‐Jin Park, Seung Hyun Han   +13 more
wiley   +1 more source

I-SceI-mediated double-strand break does not increase the frequency of homologous recombination at the Dct locus in mouse embryonic stem cells. [PDF]

PLoS ONE, 2012
Targeted induction of double-strand breaks (DSBs) at natural endogenous loci was shown to increase the rate of gene replacement by homologous recombination in mouse embryonic stem cells.
Myriam Fenina, Dominique Simon-Chazottes, Sandrine Vandormael-Pournin, Jihane Soueid, Francina Langa, Michel Cohen-Tannoudji, Bruno A Bernard, Jean-Jacques Panthier   +7 more
doaj   +1 more source

A Synthetic Platform for Antibody Junctional Diversification Beyond Natural Constraints

Advanced Science, EarlyView.
ARESEC is a synthetic platform that reconstructs functional V(D)J recombination in HEK293T cells. By introducing RSS elements and co‐expressing RAG1/2 and TdT across all three antibody CDRs, it enables programmable junctional diversification and direct generation of high‐affinity variants targeting PD‐1/PD‐L1, overcoming the evolutionary constraints of
Wang Liu, Jun Yang, Yuhang Zhang, Xiaoli Xue   +3 more
wiley   +1 more source

HO Endonuclease-Initiated Recombination in Yeast Meiosis Fails To Promote Homologous Centromere Pairing and Is Not Constrained To Utilize the Dmc1 Recombinase

G3: Genes, Genomes, Genetics, 2018
Crossover recombination during meiosis is accompanied by a dramatic chromosome reorganization. In Saccharomyces cerevisiae, the onset of meiotic recombination by the Spo11 transesterase leads to stable pairwise associations between previously ...
Lina Yisehak, Amy J. MacQueen
doaj   +1 more source

Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeast Candida intermedia [PDF]

, 2023
International audienceAbstract Genome editing toolboxes are essential for the exploration and exploitation of non-conventional yeast species as cell factories, as they facilitate both genome studies and metabolic engineering.
Peri, Kameshwara, Plovie, Alexander, Papon, Nicolas, Geijer, Cecilia, Da Silva Faria Oliveira, Fábio Luis,, Faria-Oliveira, Fábio, Peri, Kameshwara Venkata Ramana, Peri, Kameshwara Venkata Ramana,, Plovie, Alexander,, Da Silva Faria Oliveira, F\ue1bio Luis, Larsson, Adam, Geijer, Cecilia,, Larsson, Adam,   +12 more
core   +1 more source

Synthetic Shewanella‐Bacillus Microbial Consortia for Enhanced Metal Oxide Leaching From Spent Lithium‐Ion Batteries Using Brewing Wastewater as Leaching Agent

Advanced Science, EarlyView.
A recycling approach of spent lithium‐ion batteries is developed using brewing wastewater as the leaching agent and an engineering microbial consortium as the biocatalyst. Shewanella oneidensis reduces spent LiNixCoyMn1‐x‐yO2 cathodes via extracellular electron transfer, while Bacillus subtilis removes residual ethanol to eliminate inhibition.
Baocai Zhang, Chunxiao Qiao, Huichun Liu, Yiyun Wang, Yan Liu, Huan Yu, Junqi Zhang, Chao Li, Deguang Wu, Qulai Tang, Zhiwen Wang, Danni Zhang, Dake Xu, Feng Li, Hao Song   +14 more
wiley   +1 more source

The dynamics of homologous pairing during mating type interconversion in budding yeast. [PDF]

PLoS Genetics, 2006
Cells repair most double-strand breaks (DSBs) that arise during replication or by environmental insults through homologous recombination, a high-fidelity process critical for maintenance of genomic integrity.
Peter L Houston, James R Broach
doaj   +1 more source

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells [PDF]

Nucleic Acids Research, 2003
Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site- specific DNA double-strand breaks (DSBs) have been used to induce efficient gene targeting. Engineering highly specific, dedicated DNA endonucleases is the key to a wider usage of this technology.
Jean-Charles, Epinat, Sylvain, Arnould, Patrick, Chames, Pascal, Rochaix, Dominique, Desfontaines, Clémence, Puzin, Amélie, Patin, Alexandre, Zanghellini, Frédéric, Pâques, Emmanuel, Lacroix   +9 more
openaire   +2 more sources

Design of a Novel Diamine Biosensor to Guide the Engineering of Escherichia coli for Cadaverine Overproduction

Advanced Science, EarlyView.
A high‐throughput screening platform was established by integrating an in vivo cadaverine biosensor with a fluorescence‐activated cell sorting (FACS) system. Biosensor‐driven specific exporter tuning, pathway optimization and genome‐wide target mining were conducted to maximize cadaverine production in Escherichia coli.
Jia Feng, Getao Liu, Ding Ma, Jiaxiang Yuan, Xinyi Zhang, Weihao Wang, Chen Ma, Yang Liao, Siyuan Gao, Lei Qin, Jiao Feng, Sheng Xu, Xin Wang, Kequan Chen   +13 more
wiley   +1 more source