Results 211 to 220 of about 1,465,723 (294)

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

REAP1/AtSWAP70 integrates RAB5 and ROP signalling during sexual reproduction. [PDF]

Nat Plants
Ito E, Rzepecka NJ, Ito Y, Hirano T, Ebine K, Oda Y, Sato MH, Nakano A, Uemura T, Ueda T.   +9 more
europepmc   +1 more source

Magnetic Particle‐Based Thin‐Channel Analysis for Separation and Bioanalysis: From SPLITT Fractionation to Immunoassays

Journal of the Chinese Chemical Society, EarlyView.
Thin‐channel magnetic analytical platforms integrate magnetically controlled transport with confined laminar flow to enable quantitative bioanalysis. By coupling field‐induced migration with outlet fractionation, particle deposition, or affinity capture, these platforms merge SPLITT separation, magnetophoresis, and magnetic immunoassays.
Hweiyan Tsai, Jia‐Yun Lyu, C. Bor Fuh
wiley   +1 more source

Multifunctional Bioactivity of <i>Saccharomyces cerevisiae</i> Extracellular Vesicle in Hair Follicle-Related Cellular Models. [PDF]

Molecules
Park HS, Shin E, Shin S.
europepmc   +1 more source

Production of recombinant human granulocyte‐macrophage colony‐stimulating factor in thermoinducible E. coli: Tuning inclusion body architecture and protein yield via dissolved oxygen control

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Recombinant protein expression in Escherichia coli frequently results in the formation of inclusion bodies (IBs), which provide high protein accumulation but often require additional steps to recover functional protein. Although culture conditions strongly influence IB physicochemical and structural characteristics, the impact of ...
Ximena Gómez‐Gil, Norma A Valdez‐Cruz, Sara Restrepo‐Pineda, Nuria Sánchez‐Puig, Rafael Vázquez‐Duhalt, Rubén D Cadena‐Nava, Néstor O Pérez, Mauricio A Trujillo‐Roldán   +7 more
wiley   +1 more source

Biosurfactant‐assisted recovery and remediation of hydrocarbons from oily sludge using a mobile aerated washing system

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND The oil industry generates oily sludge, the accumulation of which poses a serious environmental problem. Conventional methods require high costs, specialized equipment, and qualified personnel. This waste can be treated using biosurfactants.
Maria Eduarda Pereira da Silva, Fabíola Carolina Gomes de Almeida, Gabriel Neves da Motta Silveira, Bruno Humberto de Almeida Silva, Maria da Glória Conceição da Silva, Celmy Maria Bezerra de Menezes Barbosa, Leonie Asfora Sarubbo   +6 more
wiley   +1 more source

Arabidopsis EMB2788/Npa1L1 is critical for pre-rRNA processing, ribosome biogenesis, and embryo development. [PDF]

Plant J
Liu M, Wang S, Li Z, Liu Z, Tong M, Zhang A, Chen N, Zhu Y, Zhang J, Chen L, Guo Y, Li R.   +11 more
europepmc   +1 more source

Balancing Prediction Performance and Spectral Interpretability: Multimetric Guided Design of Raman Spectrum Preprocessing Pipelines to Transfer Single Compound Models to Bioprocess Data

Journal of Raman Spectroscopy, EarlyView.
Calibration models were trained using single‐compound Raman spectra of ethanol and glucose, and then transferred to bioprocess spectra. Preprocessing, optimized to meet a multimetric quality framework, enabled this transfer whilst preserving the characteristic bands and the chemical interpretability of the models.
Gianmarco Luchetti Sfondalmondo, Marieke E. Klijn   +1 more
wiley   +1 more source

Native yeast kinetochore structures identify an essential inner kinetochore interaction. [PDF]

Nat Commun
Jiang M, Hu C, Hedouin S, Andrade Latino A, Arimura Y, Stergachis AB, Biggins S.   +6 more
europepmc   +1 more source

Sensory and chemical profiles of bread derived from the novel semi‐ and wholegrain flour enrichment of Triticum aestivum L. old genotypes with organic stinging nettle

JSFA reports, Volume 5, Issue 3, Page 104-112, March 2025.
Abstract Background Bread made from refined Triticum aestivum L. flour, a staple food in large parts of the world, is considered a nutrient security risk. Strategies used to enhance the nutritional and functional properties of wheat‐derived food products include the use of semi‐ or wholegrain flour, old genotypes, and enrichment through fortification ...
Ilaria Marotti, Elettra Frassineti, Mattia Alpi, Giovanni Dinelli   +3 more
wiley   +1 more source