Results 251 to 260 of about 1,465,723 (294)

Synthesis and assembly of human vault particles in yeast

Biotechnology and Bioengineering, 2018
AbstractVault particles are the largest naturally occurring ribonucleoprotein complexes found in the cytoplasm. In all 78 copies of major vault protein (MVP) assemble on polyribosome templates, forming recombinant vault particles, which are of great interest as encapsulation carriers for therapeutics delivery and enzyme stabilization.
Meng Wang, Valerie A. Kickhoefer, Leonard H. Rome, Oliver K. Foellmer, Shaily Mahendra   +4 more
openaire   +2 more sources

Yeast Retrotransposon Particles as Antigen Delivery Systems

Annals of the New York Academy of Sciences, 1995
The development of technologies to produce recombinant proteins for use in the pharmaceutical industry has made substantial advances, in particular in the area of generating antigens containing multiple copies of important immunological regions. One such antigen-carrier system is based on the ability of a protein encoded by the yeast retrotransposon ...
A J, Kingsman, N R, Burns, G T, Layton, S E, Adams   +3 more
openaire   +2 more sources

Yeast nucleosomal particles: structural and transcriptional properties

Biochemistry, 1991
Yeast nucleosomal core particles have been characterized by thermal denaturation, circular dichroism, and digestion with DNase I and with trypsin. Practically all nucleosomal DNA melts in one transition centered at 70 degrees C, and the circular dichroism spectrum is displaced to lower wavelengths as compared to that corresponding to chicken ...
M, Piñeiro, C, Puerta, E, Palacián
openaire   +2 more sources

The yeast Ty virus-like particles

Yeast, 2000
Virus-like particle (VLP) assembly is a crucial step of the life cycle of retrotransposons. The S. cerevisiae Ty elements represent an interesting model for the analysis of these particles and thus have been studied extensively. Our current knowledge of the organisation and assembly of Ty1 and Ty3 VLPs is reviewed here.
openaire   +2 more sources

Maturation of pre‐40S particles in yeast and humans

WIREs RNA, 2018
The synthesis of ribosomal subunits in eukaryotes requires the interplay of numerous maturation and assembly factors (AFs) that intervene in the insertion of ribosomal proteins within pre‐ribosomal particles, the ribosomal subunit precursors, as well as in pre‐ribosomal RNA (rRNA) processing and folding.
Emilie, Cerezo, Célia, Plisson-Chastang, Anthony K, Henras, Simon, Lebaron, Pierre-Emmanuel, Gleizes, Marie-Françoise, O'Donohue, Yves, Romeo, Yves, Henry   +7 more
openaire   +2 more sources

Generation of Sendai Virus Nucleocapsid-Like Particles in Yeast

Virus Research, 2005
The gene encoding Sendai virus nucleocapsid protein was cloned into the yeast Saccharomyces cerevisiae expression vector pFGG3 under control of GAL7 promoter. The high level of recombinant Sendai virus nucleocapsid protein expression (12-14 mg/l of yeast culture) was obtained.
Takemasa Sakaguchi, Mindaugas Juozapaitis, Rimantas Slibinskas   +2 more
exaly   +3 more sources

Virus-like particles in yeast: Isolation and infectivity

Zeitschrift für allgemeine Mikrobiologie, 1978
Virus-like particles containing electron dense cores are seen in thin sections of intact and degenerated cells of a thermosensitive (ts) strain of Candida tropicalis. A particulate fraction not present in wild-type cells has been isolated from the ts cells disrupted by pressure. The particles are 80-120 nm in diameter.
G F, Nesterova, J, Kärner, Y, Soom, T M, Kozlova   +3 more
openaire   +2 more sources

A Protein Transformation Protocol for Introducing Yeast Prion Particles into Yeast

2010
A range of methods for transforming organisms with nucleic acids has been established. However, techniques for introducing proteins, or particularly protein aggregates, into cells are less developed. Here, we introduce a highly efficient protocol for introducing protein aggregates such as prions into yeast.
openaire   +2 more sources

Deposition of composite coatings from particle–particle and particle–yeast blends by convective-sedimentation assembly

Journal of Colloid and Interface Science, 2012
The structures resulting from convective-sedimentation assembly (CSA) of bimodal suspensions (4.1-10% solids) of strongly charged sulfate latex microspheres (zeta potential -55.9±1.8 mV at pH 8.0) and weakly charged Saccharomyces cerevisiae (zeta potential -18.7±0.71 mV at pH 8.0) on glass, polyester, polypropylene, and aluminum foil substrates was ...
Jessica S, Jenkins, Michael C, Flickinger, Orlin D, Velev   +2 more
openaire   +2 more sources

Chromosome-like particles during meiosis in fission yeast

Archives of Microbiology, 1974
Chromosome-like structures were observed in spheroplasts ofSchizosaccharomyces pombe shortly before, or after the first meiotic division. From the work at hand, a number of six is considered to be the best estimate of a haploid set of chromosomes in this yeast.
R, Egel, A, Pentzos-Daponte
openaire   +2 more sources