Results 91 to 100 of about 1,794,891 (330)

Engineered Foxp1high Exosomes Ameliorates Systemic Lupus Erythematosus

Advanced Science
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by immune dysregulation and impaired Treg cell differentiation. Mesenchymal stem cell‐derived exosomes (MSC‐exos) hold promise for treating immune‐related diseases, while their ...
Luhan Niu, Qianmin Ou, Qianhui Ren, Zhengshi Li, Hongcheng Chen, Fangcao Lei, Xueli Mao, Songtao Shi, Zetao Chen, Wei Teng   +9 more
doaj   +1 more source

Recent advances in keratinase production via protein engineering, breeding, and fermentation

Advanced Agrochem
The gene editing and synthetic biological tools have led to the implementation of diverse metabolic engineering approaches to enhance the production of specific enzymes. Microbial keratinases can convert keratin wastes into valuable compounds for mankind.
Ali Raza Ishaq, Zheng Zhang, Penghui He, Min Xiong, Shouwen Chen   +4 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Structure-Guided Engineering of the Homodimeric Mango-IV Fluorescence Turn-on Aptamer Yields an RNA FRET Pair [PDF]

, 2020
Robert J. Trachman, Razvan Cojocaru, Di Wu, Grzegorz Piszczek, Michaël Ryckelynck, Peter J. Unrau, A.R. Ferré-D′Amaré   +6 more
openalex   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

The GOODSTEP project: General Object-Oriented Database for Software Engineering Processes [PDF]

, 1994
The goal of the GOODSTEP project is to enhance and improve the functionality of a fully object-oriented database management system to yield a platform suited for applications such as software development environments (SDEs).
Abiteboul, S, Adiba, M, Arlow, J, Armenise, P, Bandinelli, S, Baresi, L, Breche, P, Buddrus, F, Collet, C, Collet, P, Corte, P, Coupaye, TH, Delobel, C, Emmerich, W, Ferran, G, Ferrandina, F, Fuggetta, A, Ghezzi, C, Lautemann, SE, Lavazza, L, Madec, J, Phoenix, M, Sachweh, S, Schaefer, W, Souza dos Santos, C, Tigg, G, Zicari, R   +26 more
core  

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Strong modulation of optical properties in black phosphorus through strain-engineered rippling

, 2016
Controlling the bandgap through local-strain engineering is an exciting avenue for tailoring optoelectronic materials. Two-dimensional crystals are particularly suited for this purpose because they can withstand unprecedented non-homogeneous deformations
Agraït, Nicolás, Castellanos-Gomez, Andres, Guinea, Francisco, Molina-Mendoza, Aday, Parente, Vincenzo, Quereda, Jorge, Roldán, Rafael, Rubio-Bollinger, Gabino, San-José, Pablo, Vaquero-Garzon, Luis   +9 more
core   +2 more sources

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing [PDF]

, 2021
Brian R. Shy, Vivasvan S. Vykunta, Alvin Ha, Theodore L. Roth, Alexis Talbot, David N. Nguyen, Yan Yi Chen, Franziska Blaeschke, Shane Vedova, Murad R. Mamedov, Jing-Yi Chung, Hong Li, Jeffrey L. Wolf, Thomas Martin, Lumeng Ye, Justin Eyquem, Jonathan H. Esensten, Alexander Marson   +17 more
openalex   +1 more source