Results 51 to 60 of about 3,511,649 (249)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Yield and tensile strength of vinyl ester composites cured by microwaves

, 2007
In the previous investigation, it was found that the difference in Young’s modulus, ultimate tensile strength and yield strength between microwave cured vinyl ester particulate composites and those cured under ambient conditions is minimal provided low ...
Trada, M., Ku, H., Kota, V., Puttgunta, V. C.   +3 more
core   +1 more source

Flexural Behavior of Beams Reinforced with Steel Bars Exceeding the Nominal Yield Strength

Latin American Journal of Solids and Structures
If steel manufactures usually comply with the minimum code specifications, the nominal yield strength of rebar can however be significantly exceeded in many countries, depending on the steel manufacturing processes. Such an increase in yield strength can
Abdelhamid Charif, Shehab M. Mourad, M. Iqbal Khan   +2 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

An instrumented indentation method for evaluating the effect of hydrostatic pressure on the yield strength of solid polymers

, 2014
The yield behavior of solid polymers may be influenced by the hydrostatic pressure, strain rate, and temperature. In the present work, we focus on evaluating the effect of hydrostatic pressure on the yield strength by instrumented indentation.
冯义辉, Wen DH, Peng GJ, 张泰华, Zhang, TH (reprint author), Zhejiang Univ Technol, Coll Mech Engn, Hangzhou 310014, Zhejiang, Peoples R China.   +4 more
core   +1 more source

The impact of annealing temperatures and chemical assays on the mechanical properties and electrical conductivity of CuNi9Sn2 [PDF]

Mining and Metallurgy Engineering Bor
The interest in copper-based alloys with nickel and tin is constantly increasing. In this regard, certain tests were performed on CuNi9Sn2 alloy samples.
Ivanović Aleksandra, Dimitrijević Silvana, Dimitrijević Stevan, Mir Shaila   +3 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Acute and delayed effects of a resistance training session leading to muscular failure on mechanical, metabolic, and perceptual responses

, 2018
Párraga-Montilla, JA, García-Ramos, A, Castaño-Zambudio, A, Capelo-Ramírez, F, González-Hernández, JM, Cordero-Rodríguez, Y, and Jiménez-Reyes, P. Acute and delayed effects of a resistance training session leading to muscular failure on mechanical ...
Capelo Ramírez, Fernando, Cordero Rodríguez, Yaiza Adela, Párraga Montilla, Juan A, García Ramos, Amador, Castaño Zambudio, Adrián, Jiménez Reyes, Pedro, González Hernández, Jorge M   +6 more
core   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source