Versatile Detection of Cellular Protein via Fluorescence Anisotropy
Cell Lysate Fluorescence Anisotropy (CFAST) utilizes long lifetime dye‐labeled nanobodies for rapid protein quantification in minimally purified, complex cell lysate. When coupled with cellular thermal shift, CFAST allows high‐throughput detection of endogenous protein‐small molecule engagement, facilitating the discovery of novel binders for ...
Qing Tang +12 more
wiley +1 more source
Correction: Li et al. Effect of Interlayer Temperature-Controlled Thermal Cycling on the Microstructure and Mechanical Properties of Wire Arc Directed Energy Deposition H13 Steel. <i>Materials</i> 2026, <i>19</i>, 111. [PDF]
Li C +8 more
europepmc +1 more source
Single‐cell RNA editing analysis identifies ADAR1 as a regulator of dysfunctional T cell states in colorectal cancer. Elevated ADAR1 activity promotes T cell exhaustion and impairs antitumor immunity partly through TGF‐β‐SMAD signaling, contributing to anti‐PD‐1 resistance and highlighting T cell ADAR1 as a potential therapeutic target and biomarker ...
Da Kang +10 more
wiley +1 more source
MIS12 Is Required for Kinetochore‐Microtubule Attachment in Oocyte Meiosis
A model depicting the role of MIS12 in K‐MT attachment during oocyte meiosis. The presence of MIS12 stabilizes bipolar K‐MT attachments by maintaining the function of NDC80 and its interaction with TUBB. This, in turn, promotes KNL1 assembly and subsequent SAC protein recruitment to kinetochores.
Jian Li +9 more
wiley +1 more source
Correction: Characteristic analysis of the chloroplast genome of <i>Cyathula capitata</i> and comparative analysis with <i>Cyathula officinalis</i> and their hybrid <i>Cyathula officinalis</i> × <i>Cyathula capitata</i>. [PDF]
Gao B +6 more
europepmc +1 more source
This study identifies C4 as a lead inhibitor of the Lpt system. Notably, C4 potentiates colistin activity by disrupting LPS transport and remodeling phospholipid homeostasis, revealing a functional interplay between the Lpt and Mla systems. These findings establish a mechanistic link between Lpt inhibition and membrane lipid remodeling, positioning Lpt–
Jianya Luo +6 more
wiley +1 more source
Correction to 'The European Nucleotide Archive in 2025'. [PDF]
europepmc +1 more source
Correction: Study on the mechanism of 18β-glycyrrhetinic acid inhibiting the proliferation of renal cancer cells by inducing autophagy through the miR-27a-5p/LC3 axis. [PDF]
Jia S +9 more
europepmc +1 more source
An intelligent odor monitoring system integrates an IVC animal model, gas sensor array, real‐time resistance readout, and machine‐learning analysis to continuously monitor infection‐associated odor changes. The platform captures longitudinal sensor responses, distinguishes infected from healthy states, and supports early‐stage respiratory viral ...
Yajie Shen +17 more
wiley +1 more source

