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Genetic modification of Zymomonas mobilis

Biotechnology Advances, 1990
The bacterium Zymomonas mobilis is a potentially useful organism for the commercial production of ethanol as it is capable of more than double the rate of alcohol production by yeast. However, industrial application of this bacterium has been restricted in part due to the disadvantages of its limited substrate range (glucose, fructose and sucrose) and ...
S E, Buchholz, D E, Eveleigh
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Metabolism of galactose in Zymomonas mobilis

Applied Microbiology and Biotechnology, 1991
Zymomonas mobilis IFO 13 756 could take up galactose and metabolize it so that galactonic acid accumulated in the culture fluid. The oxidation of galactose was catalysed by a membrane-associated enzyme, which could be solubilized with a mixture of 1% Triton X-100 and 1 M: KC1. The strain also accumulated galactitol as a minor metabolite.
Hideshi, Yanase   +4 more
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Fructose Phosphorylation by Zymomonas mobilis Glucokinase

Acta Biotechnologica, 1990
AbstractZymomonas mobilis ATCC 53431, a fructokinase negative mutant is unable to utilize fructose as a sole carbon source for growth. At high fructose concentrations, however, fructose was converted to ethanol. The fructose uptake displayed MICHAELIS‐MENTEN kinetics with an apparent Km of 185 mM fructose.Purified glucokinase from ATCC 53431 and the ...
Edye L.A., Kositanont C., Doelle H.W.
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Comparison of plasmids in strains of Zymomonas mobilis

Plasmid, 1983
Four strains of Zymomonas mobilis were examined for their resistance to antimicrobial agents and found to have similar resistance profiles. Plasmid DNA was extracted and purified by CsCl dye-buoyant density centrifugation; molecular weights were determined by agarose gel electrophoresis and electron microscopy. All four strains harbored a large plasmid
H W, Stokes   +3 more
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Fructose metabolism in Zymomonas mobilis

Applied Microbiology and Biotechnology, 1986
In the metabolism of fructose by Zymomonas, the ethanol yield is decreased due to the formation of dihydroxyacetone, mannitol and glycerol. The reduction of fructose to mannitol by an NADPH-dependent mannitol dehydrogenase is apparently coupled to the oxidation of glucose-6-phosphate by glucose-6-phosphate dehydrogenase, which exhibits higher activity ...
Korhola, Matti, Viikari, Liisa
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Transformation of Zymomonas mobilis by a hybrid plasmid

Plasmid, 1984
The transformation of Zymomonas mobilis by plasmid DNA was achieved using a modification of the CaCl2 method for Escherichia coli. The highest frequency of transformation obtained was 5 X 10(3) transformants/micrograms DNA. The success of the method depended upon the use of a plasmid which is a cointegrate between a Z. mobilis cryptic plasmid and an E.
G M, Browne   +3 more
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Zymomonas mobilis: A bacterium for ethanol production

Biotechnology Advances, 1986
Biochimie generale et metabolisme. Ecologie et morphologie. Aspects cinetiques. Fermentation.
J C, Baratti, J D, Bu'lock
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Bioethanol production from algae Spirogyra hyalina using Zymomonas mobilis [PDF]

open access: yesBiofuels, 2016
Algae Spirogyra have the potential to be developed as a raw material for bioethanol production. This study aims to determine the effect of the hydrolysis process of algae Spirogyra on total sugar production.
Sutiman Sumitro, Mohamad Amin
exaly   +2 more sources

Formation of sorbitol by Zymomonas mobilis

Applied Microbiology and Biotechnology, 1984
Sorbitol is formed as the major by-product in ethanol fermentations by Zymomonas mobilis when both glucose and fructose are present in the fermentation medium. The amount of sorbitol produced was equivalent to as much as 11% of the original carbon source, decreasing the ethanol yield correspondingly.
openaire   +3 more sources

Sorbitol production by Zymomonas mobilis

Applied Microbiology and Biotechnology, 1984
High resolution 13C Nuclear Magnetic Resonance (NMR) spectroscopy has been employed to determine the chemical composition of the unknown major products in a sucrose or fructose plus glucose fermentation to ethanol by the bacterium Zymmonas mobilis. When grown on these sugars Z.mobilis was found to produce significant amounts of sorbitol, up to 43 g·l-1
KevinD. Barrow   +4 more
openaire   +1 more source

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