Results 11 to 20 of about 185,490 (288)

Synthesis of Bioconjugation Reagents for Use in Covalent Cross-Linking of Proteins by Azide-Alkyne Cycloaddition [PDF]

Molecules
A series of azide- and cyclooctyne-functionalized N-hydroxysuccinimidyl esters (NHS esters) and benzotriazolides were prepared and used as N-acylation reagents to obtain azide-(BSA-1) and cyclooctyne-functionalized bovine serum albumin proteins (BSA-2 ...
Nadja Suhorepec, Luka Ciber, Uroš Grošelj, Nejc Petek, Bogdan Štefane, Marko Novinec, Jurij Svete   +6 more
doaj   +2 more sources

Insights into Transient Dimerisation of Carnitine/Acylcarnitine Carrier (SLC25A20) from Sarkosyl/PAGE, Cross-Linking Reagents, and Comparative Modelling Analysis [PDF]

Biomolecules
The carnitine/acylcarnitine carrier (CAC) is a crucial protein for cellular energy metabolism, facilitating the exchange of acylcarnitines and free carnitine across the mitochondrial membrane, thereby enabling fatty acid β-oxidation and oxidative ...
Nicola Giangregorio, Annamaria Tonazzi, Ciro Leonardo Pierri, Cesare Indiveri   +3 more
doaj   +2 more sources

Cysteine-enabled cleavability to advance cross-linking mass spectrometry for global analysis of endogenous protein-protein interactions [PDF]

Nature Communications
Cross-linking mass spectrometry (XL-MS) is a powerful technology for probing protein-protein interactions (PPIs) and elucidating architectures of protein complexes at the systems level. While successful, the proteome coverage remains limited.
Fenglong Jiao, Merav Braitbard, Clinton Yu, Ben Shor, Bjorn-Erik Wulff, Dina Schneidman-Duhovny, Lan Huang   +6 more
doaj   +2 more sources

Trioxane-based MS-cleavable cross-linking mass spectrometry for profiling multimeric interactions of cellular networks [PDF]

Nature Communications
Cross-linking mass spectrometry (XL-MS) is a powerful technology for mapping protein-protein interactions (PPIs) at the systems level. While bivalent cross-links are effective for defining protein interactions and structures, multivalent cross-links ...
Clinton Yu, Eric Novitsky, Sree Ganesh Balasubramani, Xiaorong Wang, Xiyu Shen, Qin Yang, Scott Rychnovsky, Ignacia Echeverria, Lan Huang   +8 more
doaj   +2 more sources

Reversible cross-linking of gelatin by a disulphide-containing bis-succinimide for tunable degradation and release

Food Chemistry: X, 2023
Generally, gelatin was irreversibly cross-linked by chemical reagents to improve its water-resistance. However, few chemical reagents meet both the requirements of high cross-inking efficiency and tunable degradation.
Shengbin He, Jingtong Wang, Zhao Li, Yongqiang Cao, Xueping Ning, Jian Sun, Quanzhi Chen, Min Ling   +7 more
doaj   +1 more source

Reactive Acrylamide-Modified DNA Traps for Accurate Cross-Linking with Cysteine Residues in DNA–Protein Complexes Using Mismatch Repair Protein MutS as a Model

Molecules, 2022
Covalent protein capture (cross-linking) by reactive DNA derivatives makes it possible to investigate structural features by fixing complexes at different stages of DNA–protein recognition.
Mayya V. Monakhova, Elena A. Kubareva, Kirill K. Kolesnikov, Viktor A. Anashkin, Egor M. Kosaretskiy, Maria I. Zvereva, Elena A. Romanova, Peter Friedhoff, Tatiana S. Oretskaya, Timofei S. Zatsepin   +9 more
doaj   +1 more source

Mimicked synthetic ribosomal protein complex for benchmarking crosslinking mass spectrometry workflows

Nature Communications, 2022
Cross-linking mass spectrometry is widely used to elucidate protein structures and interactions. Here, the authors generate an extensive peptide library to benchmark the most common cross-link search engines with frequently used cross-linking reagents in
Manuel Matzinger, Adrian Vasiu, Mathias Madalinski, Fränze Müller, Florian Stanek, Karl Mechtler   +5 more
doaj   +1 more source

Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review

Nanomaterials, 2023
The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability
Alexandra Virginia Bounegru, Constantin Apetrei   +1 more
doaj   +1 more source

Ligand-induced movements of inner transmembrane helices of Glut1 revealed by chemical cross-linking of di-cysteine mutants. [PDF]

PLoS ONE, 2012
The relative orientation and proximity of the pseudo-symmetrical inner transmembrane helical pairs 5/8 and 2/11 of Glut1 were analyzed by chemical cross-linking of di-cysteine mutants.
Mike Mueckler, Carol Makepeace
doaj   +1 more source

Application of proanthocyanidin in the treatment and repair of deep caries

口腔疾病防治, 2023
Proanthocyanidin (PA), as a kind of natural plant polyphenol, have a variety of biological functions, such as promoting remineralization, inducing collagen cross-linking, inhibiting protease activity and inhibiting bacteria.
GAO Ying, WANG Jue, TAO Lu, YAO Jingjing, YU Jinling , HUANG Handan, YANG Liuqing, LIU Zhihui   +7 more
doaj   +1 more source