Covalent cross‐linking of proteins without chemical reagents [PDF]
Protein Science, 2002 AbstractA facile method for the formation of zero‐length covalent cross‐links between protein molecules in the lyophilized state without the use of chemical reagents has been developed. The cross‐linking process is performed by simply sealing lyophilized protein under vacuum in a glass vessel and heating at 85°C for 24 h.
Brigitte Simons +4 more
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Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review
Nanomaterials, 2023 The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability
Alexandra Virginia Bounegru +1 more
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PhoX – an IMAC-enrichable Crosslinking Reagent [PDF]
ACS Central Science, 2019 AbstractChemical crosslinking mass spectrometry is rapidly emerging as a prominent technique to study protein structures. Structural information is obtained by covalently connecting peptides in close proximity by small reagents and identifying the resulting peptide pairs by mass spectrometry.
Steigenberger, Barbara A. +3 more
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Application of proanthocyanidin in the treatment and repair of deep caries
口腔疾病防治, 2023 Proanthocyanidin (PA), as a kind of natural plant polyphenol, have a variety of biological functions, such as promoting remineralization, inducing collagen cross-linking, inhibiting protease activity and inhibiting bacteria.
GAO Ying +7 more
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A new radioactive cross-linking reagent for studying the interactions of proteins. [PDF]
Journal of Biological Chemistry, 1982 We have developed a photoactivable, heterobifunctional, reversible, radioactively labeled, chemical cross-linking reagent, 3-[(2-nitro-4-azidophenyl)-2-aminoethyldithio]-N-succinimidyl propionate, for studying the interactions of proteins in situ. When reacted in the dark with a purified protein, it forms a covalent derivative which can be purified and
Martin A. Schwartz +2 more
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ECL 3.0: a sensitive peptide identification tool for cross-linking mass spectrometry data analysis
BMC Bioinformatics, 2023 Background Cross-linking mass spectrometry (XL-MS) is a powerful technique for detecting protein–protein interactions (PPIs) and modeling protein structures in a high-throughput manner.
Chen Zhou +6 more
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The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents [PDF]
, 1990 Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 34-kD integral membrane glycoprotein (Wiedmann, M., T. V. Kurzchalia, E. Hartmarm, and T. A. Rapoport. 1987. Nature
Dobberstein, Bernhard +9 more
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Mass Spectrometry Evidence for Cisplatin As a Protein Cross-Linking Reagent [PDF]
Analytical Chemistry, 2011 Cisplatin is a potent anticancer drug, which functions by cross-linking adjacent DNA guanine residues. However within 1 day of injection, 65-98% of the platinum in the blood plasma is protein-bound. It is generally accepted that cisplatin binds to methionine and histidine residues, but what is often underappreciated is that platinum from cisplatin has ...
Huilin Li +6 more
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Cleavage and cross-linking of proteins with osmium (VII) reagents. [PDF]
Journal of Histochemistry & Cytochemistry, 1982 The specific cleavage of proteins following reaction with osmium tetroxide is slowed down by tertiary amines which are known to be good ligands for osmium. Bidentate ligands are more effective than monodentate ligands. These same ligands also promote an intermolecular cross-linking reaction.
E. J. Behrman, Michael Emerman
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Antigenic Properties of Fixed and Unfixed Particles of Some Cucumber Mosaic Virus Strains [PDF]
E3S Web of Conferences, 2020 The virus particle of Cucumber mosaic virus (CMV) is unstable and degrades during and after virus preparation. For longterm storage, either 0.25% glutaraldehyde or 0.2% formaldehyde are used as protein cross-linking reagents for stabilising the antigenic
Sri Wahyuni Wiwiek
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