Results 11 to 20 of about 40,389 (237)

Wrapping Pathways of Anisotropic Dumbbell Particles by Giant Unilamellar Vesicles. [PDF]

Nano Lett, 2023
Endocytosis is a key cellular process involved in the uptake of nutrients, pathogens or the diagnosis and therapy of diseases. Most studies have focused on spherical objects, whereas biologically relevant shapes can be highly anisotropic. In this letter, we use an experimental model system based on Giant Unilamellar Vesicles (GUVs) and dumbbell-shaped ...
Azadbakht A, Meadowcroft B, Varkevisser T, Šarić A, Kraft DJ.   +4 more
europepmc   +10 more sources

Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles [PDF]

Pharmaceutics, 2022
In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-
Paola Albanese, Simone Cataldini, Chloe Z.-J. Ren, Nadia Valletti, Jlenia Brunetti, Jack L.-Y. Chen, Federico Rossi   +6 more
doaj   +6 more sources

Bulk Self-Assembly of Giant, Unilamellar Vesicles. [PDF]

ACS Nano, 2020
The desire to create cell-like models for fundamental science and applications has spurred extensive effort towards creating giant unilamellar vesicles (GUVs). However, a route to selectively self-assemble GUVs in bulk has remained elusive. In bulk solution, membrane-forming molecules such as phospholipids, single-tailed surfactants, and block ...
Kindt JT, Szostak JW, Wang A.
europepmc   +7 more sources

Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles [PDF]

Advanced Science, 2023
In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic ...
Jorik Waeterschoot, Willemien Gosselé, Hojjat Alizadeh Zeinabad, Jeroen Lammertyn, Erin Koos, Xavier Casadevall i Solvas   +5 more
doaj   +5 more sources

Engineering Phosphatidylserine Containing Asymmetric Giant Unilamellar Vesicles [PDF]

Membranes
The plasma membrane lipid distribution is asymmetric, with several anionic lipid species located in its inner leaflet. Among these, phosphatidylserine (PS) plays a crucial role in various important physiological functions.
Jake McDonough, Trevor A. Paratore, Hannah M. Ketelhohn, Bella C. DeCilio, Alonzo H. Ross, Arne Gericke   +5 more
doaj   +4 more sources

Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs) [PDF]

Scientific Reports, 2021
Target delivery of large foreign materials to cells requires transient permeabilization of the cell membrane without toxicity. Giant unilamellar vesicles (GUVs) mimic the phospholipid bilayer of the cell membrane and are also useful drug delivery ...
Diogo A. Pereira, Alexandre D. Silva, Patricia A. T. Martins, Ana P. Piedade, Dmitro Martynowych, David Veysset, Maria João Moreno, Carlos Serpa, Keith A. Nelson, Luis G. Arnaut   +9 more
doaj   +4 more sources

A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles [PDF]

Bio-Protocol, 2022
Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen, Thomas Günther Pomorski   +1 more
doaj   +2 more sources

Interplay of packing and flip-flop in local bilayer deformation. How phosphatidylglycerol could rescue mitochondrial function in a cardiolipin-deficient yeast mutant [PDF]

Biophys. J. 107(4): 879-890 (2014), 2014
In a previous work, we have shown that a spatially localized transmembrane pH gradient, produced by acid micro-injection near the external side of cardiolipin-containing giant unilamellar vesicles, leads to the formation of tubules that retract after the dissipation of this gradient. These tubules have morphologies similar to mitochondrial cristae. The
Angelova, Miglena I., Arroyo, Marino, Bitbol, Anne-Florence, Fournier, Jean-Baptiste, Khalifat, Nada, Puff, Nicolas, Rahimi, Mohammad, Seigneuret, Michel   +7 more
arxiv   +6 more sources

Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles [PDF]

Molecules, 2022
Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions.
Ogadimma D. Okagu, Raliat O. Abioye, Chibuike C. Udenigwe   +2 more
doaj   +2 more sources

Permeability of phospholipid membrane for small polar molecules determined from osmotic swelling of giant phospholipid vesicles [PDF]

Advances in Planar Lipid Bilayers and Liposomes, Volume 16, 2012, Pages 301-335, 2012
A method for determining permeability of phospholipid bilayer based on the osmotic swelling of micrometer-sized giant unilamellar vesicles (GUVs) is presented as an alternative to the two established techniques, dynamic light scattering on liposome suspension, and electrical measurements on planar lipid bilayers.
Al-Awqati, Angelova, Bangham, Bangham, Barry, Bloom, Boroske, Brunner, Carlsen, Cohen, de Gier, de Gier, Diamant, Dimova, Döbereiner, Evans, Finkelstein, Haleva, Heimburg, Heinrich, Hill, Jacobs, Karatekin, Katchalsky, Kedem, Kleinhans, Koslov, Krasteva, Mally, Mally, Mazur, Mazur, Meyer, Missner, Montal, Mueller, Nagle, Onsager, Onsager, Orbach, Overton, Paula, Pavlič, Pencer, Pencer, Peterlin, Peterlin, Peterlin, Pike, Pocivavsek, Popescu, Preston, Rawicz, Sandre, Seifert, Sha'afi, Shoemaker, Tien, Verkman, Walter, Wang, Zade-Oppen   +61 more
arxiv   +3 more sources