Results 11 to 20 of about 12,701 (197)

Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network [PDF]

BMC Bioinformatics, 2022
Background Fluorescence image analysis in biochemical science often involves the complex tasks of identifying samples for analysis and calculating the desired information from the intensity traces.
Il-Hyung Lee, Sam Passaro, Selin Ozturk, Juan Ureña, Weitian Wang   +4 more
doaj   +2 more sources

Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles [PDF]

Scientific Reports
Membrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun, Ekaterina Malysenko, Bo H. Justesen, Thomas Günther Pomorski   +3 more
doaj   +2 more sources

Block Copolymer Giant Unilamellar Vesicles for High-Throughput Screening

CHIMIA, 2022
Bottom-up synthetic cells offer the potential to study cellular processes with reduced complexity. Giant unilamellar vesicles (GUVs) can mimic cells in their morphological characteristics because their architecture is precisely controllable.
Lukas Heuberger, Cornelia Palivan
doaj   +5 more sources

Rotaxanes with a photoresponsive macrocycle modulate the lipid bilayers of large and giant unilamellar vesicles [PDF]

Communications Chemistry
Rotaxanes equipped with actuators hold great potential for developing highly functional molecular machines. Such systems could significantly enhance our ability to study and manipulate biological and artificial membranes.
Udyogi N. K. Conthagamage, Rajitha Rajeshwar T, Stijn van der Ham, Nasim Akhtar, Macallister L. Davis, Senuri G. Jayawardana, Lilia Lopez, Hanumantha Rao Vutukuri, Jeremy C. Smith, Micholas Dean Smith, Víctor García-López   +10 more
doaj   +2 more sources

Femtoliter Injection of ESCRT-III Proteins into Adhered Giant Unilamellar Vesicles [PDF]

Bio-Protocol, 2022
The endosomal sorting complex required for transport (ESCRT) machinery mediates membrane fission reactions that exhibit a different topology from that observed in clathrin-coated vesicles.
Vasil Georgiev, Yunuen Avalos-Padilla, Xavier Fernàndez-Busquets, Rumiana Dimova   +3 more
doaj   +2 more sources

Towards Better Cell Membrane Mimics: Cholesterol-Containing Supported Lipid Bilayers on TiO2 [PDF]

Biophysical Journal, 2012
Podeu consultar la versió en castellà a: http://hdl.handle.net/11703/116989Podeu consultar la versió en francès a: http://hdl.handle.net/11703 ...
Zhu, Ling, Gregurec, Danijela, Reviakine, Ilya   +2 more
core   +4 more sources

Microfluidic production, stability and loading of synthetic giant unilamellar vesicles [PDF]

Scientific Reports
In advanced drug delivery, versatile liposomal formulations are commonly employed for safer and more accurate therapies. Here we report a method that allows a straightforward production of synthetic monodisperse (~ 100 μm) giant unilamellar vesicles ...
Mart Ernits, Olavi Reinsalu, Naresh Yandrapalli, Sergei Kopanchuk, Ehsan Moradpur-Tari, Immanuel Sanka, Ott Scheler, Ago Rinken, Reet Kurg, Andreas Kyritsakis, Veikko Linko, Veronika Zadin   +11 more
doaj   +2 more sources

Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels [PDF]

Bio-Protocol, 2020
Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics.
Eric Parigoris, Daniel Dunkelmann, Unai Silvan   +2 more
doaj   +4 more sources

Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content [PDF]

Membranes
Giant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Ivan Mardešić, Zvonimir Boban, Marija Raguz   +2 more
doaj   +2 more sources

Division and Regrowth of Phase-Separated Giant Unilamellar Vesicles*. [PDF]

Angew Chem Int Ed Engl, 2021
AbstractSuccess in the bottom‐up assembly of synthetic cells will depend on strategies for the division of protocellular compartments. Here, we describe the controlled division of phase‐separated giant unilamellar lipid vesicles (GUVs). We derive an analytical model based on the vesicle geometry, which makes four quantitative predictions that we verify
Dreher Y, Jahnke K, Bobkova E, Spatz JP, Göpfrich K.   +4 more
europepmc   +7 more sources