Results 21 to 30 of about 4,881 (174)

NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: new insights for 3D domain swapping. [PDF]

PLoS ONE, 2012
Three-dimensional domain swapping is a common phenomenon in pancreatic-like ribonucleases. In the aggregated state, these proteins acquire new biological functions, including selective cytotoxicity against tumour cells.
Roberta Spadaccini, Carmine Ercole, Maria A Gentile, Domenico Sanfelice, Rolf Boelens, Rainer Wechselberger, Gyula Batta, Andrea Bernini, Neri Niccolai, Delia Picone   +9 more
doaj   +1 more source

Preliminary investigation of deoxyoligonucleotide binding to ribonuclease A using mass spectrometry: An attempt to develop a lab experience for undergraduates [version 2; referees: 2 approved]

F1000Research, 2018
Deoxyoligonucleotide binding to bovine pancreatic ribonuclease A (RNase A) was investigated using electrospray ionization ion-trap mass spectrometry (ESI-IT-MS). Deoxyoligonucleotides included CCCCC (dC5) and CCACC (dC2AC2).
Daniel D. Clark
doaj   +1 more source

Preliminary investigation of deoxyoligonucleotide binding to ribonuclease A using mass spectrometry: An attempt to develop a lab experience for undergraduates [version 1; referees: 2 approved]

F1000Research, 2018
Deoxyoligonucleotide binding to bovine pancreatic ribonuclease A (RNase A) was investigated using electrospray ionization ion-trap mass spectrometry (ESI-IT-MS). Deoxyoligonucleotides included CCCCC (dC5) and CCACC (dC2AC2).
Daniel D. Clark
doaj   +1 more source

Ribonuclease (RNase) Prolongs Survival of Grafts in Experimental Heart Transplantation

Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 2016
BackgroundCell damage, tissue and vascular injury are associated with the exposure and release of intracellular components such as RNA, which promote inflammatory reactions and thrombosis. Based on the counteracting anti‐inflammatory and cardioprotective
Eike Kleinert, Martin C. Langenmayer, Bruno Reichart, Jana Kindermann, Barbara Griemert, Andreas Blutke, Kerstin Troidl, Tanja Mayr, Tobias Grantzow, Fatih Noyan, Jan‐Michael Abicht, Silvia Fischer, Klaus T. Preissner, Ruediger Wanke, Elisabeth Deindl, Sonja Guethoff   +15 more
doaj   +1 more source

Enhancement of the activity of the antimicrobial peptides HNP1 and LL-37 by bovine pancreatic ribonuclease A [version 3; peer review: 1 approved, 2 approved with reservations, 1 not approved]

F1000Research, 2023
Background: HNP1, LL-37, and HBD1 are antimicrobial against Escherichia coli ATCC 25922 at the standard inoculum but less active at higher inocula.   Methods: The virtual colony count (VCC) microbiological assay was adapted for high inocula and the ...
Bryan Ericksen
doaj  

Localization of Disulfide Bonds in Ribonuclease Using Low pH Trypsin and LC-ESI-QTOF-MS

CHIMIA
We evaluated a method to localize disulfide bonds in bovine pancreatic ribonuclease (RNase) by applying low pH trypsin protein digestion with a bottom-up LC-ESI-QTOF-MS approach.
Amélie Bornex, Saša M. Miladinović
doaj   +1 more source

Synthetic Strategy for mRNA Encapsulation and Gene Delivery with Nanoscale Metal‐Organic Frameworks

Advanced Functional Materials, EarlyView.
This research utilizes the ZIF‐8 for the encapsulation and intracellular delivery of nucleic acids, specifically mRNA, for applications in gene delivery. Integrating PEI addresses the issue of mRNA leakage from ZIF‐8, resulting in the delivery and expression of green fluorescent protein (GFP) in vitro and firefly luciferase in vivo.
Harrison Douglas Lawson, Huy Hoang Nguyen, Keng‐Jung Lee, Nattarat Wongsuwan, Ayesha Tupe, Mengrou Lu, Mariah Lynn Arral, Anne Behre, Zihan Ling, Kathryn Ann Whitehead, Adam Walter Feinberg, Xi Ren, Si‐Yang Zheng   +12 more
wiley   +1 more source

Novel Less Toxic, Lymphoid Tissue‐Targeted Lipid Nanoparticles Containing a Vitamin B5‐Derived Ionizable Lipid for mRNA Vaccine Delivery

Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.
The top‐performing lipid nanoparticle, incorporating a novel ionizable lipid derived from vitamin B5, demonstrates high mRNA transfection efficiency, low toxicity, favorable stability, targeted delivery to lymphoid tissues, and high immunogenicity, showing promise as a carrier for mRNA vaccines for infectious diseases and cancer.
Soyeon Yoo, Muhammad Faisal, Seo‐Hyeon Bae, Kounghwa Youn, Hyo‐Jung Park, Sung Pil Kwon, Il Kwon Hwang, Jisun Lee, Hyeong Jun Kim, Jae‐Hwan Nam, Gyochang Keum, Eun‐Kyoung Bang   +11 more
wiley   +1 more source

Metal‐Responsive Up‐Regulation of Bifunctional Disulfides for Suppressing Protein Misfolding and Promoting Oxidative Folding

Angewandte Chemie, EarlyView.
A stress‐responsive folding promotor was developed by conjugating metal‐binding ligands and a redox‐active disulfide. The oxidative strength of the bifunctional disulfide was enhanced by metal coordination, inhibiting protein misfolding and up‐regulating the oxidative folding capacity under metal stress.
Keita Mori, Tsubura Kuramochi, Motonori Matsusaki, Yuki Hashiguchi, Masaki Okumura, Tomohide Saio, Yoshiaki Furukawa, Kenta Arai, Takahiro Muraoka   +8 more
wiley   +2 more sources

IncRNA‐ZFAS1, an Emerging Gate‐Keeper in DNA Damage‐Dependent Transcriptional Regulation

Advanced Science, EarlyView.
LncZFAS1 plays a crucial role during DNA damage response in mammalian cells. Loss of lncZFAS1 results in deficient DNA lesion removal and reduced cell viability. Mechanistically, lncZFAS1 modulates RNAPII phosphorylation and transcription and thereby promotes both GG‐NER and TC‐NER upon UV damage.
Jiena Liu, Qing Lu, Zixuan Fan, Jiahui Lin, Nan He, Xin Zhang, Zhaoya Han, Tingting Zhu, Zhenzhen Wu, Yingying Xu, Yuming Wang   +10 more
wiley   +1 more source