Covalent cross‐linking of proteins without chemical reagents [PDF]
Protein Science, 2002 AbstractA facile method for the formation of zero‐length covalent cross‐links between protein molecules in the lyophilized state without the use of chemical reagents has been developed. The cross‐linking process is performed by simply sealing lyophilized protein under vacuum in a glass vessel and heating at 85°C for 24 h.
Brigitte L, Simons +4 more
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PhoX – an IMAC-enrichable Crosslinking Reagent [PDF]
ACS Central Science, 2019 AbstractChemical crosslinking mass spectrometry is rapidly emerging as a prominent technique to study protein structures. Structural information is obtained by covalently connecting peptides in close proximity by small reagents and identifying the resulting peptide pairs by mass spectrometry.
Steigenberger, Barbara A. +3 more
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Antigenic Properties of Fixed and Unfixed Particles of Some Cucumber Mosaic Virus Strains [PDF]
E3S Web of Conferences, 2020 The virus particle of Cucumber mosaic virus (CMV) is unstable and degrades during and after virus preparation. For longterm storage, either 0.25% glutaraldehyde or 0.2% formaldehyde are used as protein cross-linking reagents for stabilising the antigenic
Sri Wahyuni Wiwiek
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Mass Spectrometry Evidence for Cisplatin As a Protein Cross-Linking Reagent [PDF]
Analytical Chemistry, 2011 Cisplatin is a potent anticancer drug, which functions by cross-linking adjacent DNA guanine residues. However within 1 day of injection, 65-98% of the platinum in the blood plasma is protein-bound. It is generally accepted that cisplatin binds to methionine and histidine residues, but what is often underappreciated is that platinum from cisplatin has ...
Huilin Li +6 more
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Antibodies for immunolabeling by light and electron microscopy : not for the faint hearted [PDF]
, 2014 Reliable antibodies represent crucial tools in the arsenal of the cell biologist and using them to localize antigens for immunocytochemistry is one of their most important applications.
Griffiths, Gareth, Lucocq, John Milton
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Mast cell subsets and their functional modulation by the Acanthocheilonema viteae product ES-62 [PDF]
, 2013 ES-62, an immunomodulator secreted by filarial nematodes, exhibits therapeutic potential in mouse models of allergic inflammation, at least in part by inducing the desensitisation of Fc휀RI-mediated mast cell responses.
Al-Riyami, Lamyaa +7 more
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Flexible aromatic disulfide monomers for high-performance self-healable linear and cross-linked poly(urethane-urea) coatings [PDF]
, 2019 Implementation of the self-healing concept in coatings is challenging because they have to combine mechanical strength and chain mobility. This challenge is addressed in this work by studying the effect of the polymer microstructure on the mechanical ...
Asua González, José María +4 more
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Novel microarrays for simultaneous serodiagnosis of multiple antiviral antibodies. [PDF]
PLoS ONE, 2013 We developed an automated diagnostic system for the detection of virus-specific immunoglobulin Gs (IgGs) that was based on a microarray platform. We compared efficacies of our automated system with conventional enzyme immunoassays (EIAs).
Ponnurengam Malliappan Sivakumar +5 more
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Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes
, 2014 The study of proteins and protein complexes using chemical cross-linking followed by the MS identification of the cross-linked peptides has found increasingly widespread use in recent years.
Aebersold, R. +6 more
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Enhancing caries-affected dentin bonding with a mussel-inspired primer
Frontiers in Bioengineering and BiotechnologyIntroductionDental caries is the most common oral disease. In caries-affected dentin (CAD), excessive mineral loss, extensive collagen exposure and collapse, increased enzyme activity, and bacterial residues result in significantly lower resin bonding ...
Yuntong Hu +11 more
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