Development of a Novel Sulfoxide-Containing MS-Cleavable Homobifunctional Cysteine-Reactive Cross-Linker for Studying Protein–Protein Interactions [PDF]
Cross-linking mass spectrometry (XL-MS) has become an emerging technology for defining protein-protein interactions (PPIs) and elucidating architectures of large protein complexes.
Block, Sarah A+6 more
core +2 more sources
Generally, gelatin was irreversibly cross-linked by chemical reagents to improve its water-resistance. However, few chemical reagents meet both the requirements of high cross-inking efficiency and tunable degradation.
Shengbin He+7 more
doaj
Hydrophobic adsorption and covalent immobilization of Candida antarctica lipase B on mixed-function-grafted silica gel supports for continuous-flow biotransformations [PDF]
Adsorption onto solid supports has proven to be an easy and effective way to improve the mechanical and catalytic properties of lipases. Covalent binding of lipases onto the support surface enhances the active lifetime of the immobilized biocatalysts ...
Anderson+41 more
core +1 more source
Heterobifunctional reagents for cross-linking of sugar with protein.
N-Bromoacetylsulfanilyl chloride and p-bromoacetamidobenzoyl chloride were synthesized as heterobifunctional reagents for the cross-linking of sugar with protein. These reagents react with the primary hydroxyl group of a sugar at the acid chloride group and with amino and imidazole groups of protein at the bromoacetyl group.
Mikio Ohnishi+5 more
openaire +4 more sources
INVESTIGATIONS ON THE EFFECT OF SODIUM TRIMETAPHOSPHATE AND SODIUM SULFATE CONCENTRATIONS ON THE PROPERTIES OF CROSS-LINKED CASSAVA STARCH FILMS [PDF]
Films based on pure native starch have limited applications, are easily breakable, very hygroscopic, and permeable to water vapor. One method for improving these properties is to modify starch by cross-linking.
Marisa Ferreira Karow+8 more
doaj +1 more source
Selective Enrichment and Identification of Azide-tagged Cross-Linked Peptides Using Chemical Ligation and Mass Spectrometry [PDF]
Protein-protein interaction is one of the key regulatory mechanisms for controlling protein function in various cellular processes. Chemical cross-linking coupled with mass spectrometry has proven to be a powerful method not only for mapping protein ...
Huang, Lan+4 more
core +1 more source
A novel method for tracking structural changes in gels using widely accessible microcomputed tomography is presented and validated for various hydro‐, alco‐, and aerogels. The core idea of the method is to track positions of micrometer‐sized tracer particles entrapped in the gel and relate them to the density of the gel network.
Anja Hajnal+3 more
wiley +1 more source
The properties conferred upon triple-helical collagen-mimetic peptides by the presence of cysteine residues [PDF]
Recently, the ability of polymeric collagen-like peptides to regulate cell behavior has generated great interest. A triple-helical peptide known as collagen-related peptide (CRP) contains the sequence (Gly-Pro-Hyp)10.
Bihan, Dominique G.+6 more
core +1 more source
A Comparative Study of the Characteristics of Cross-Linked, Oxidized and Dual-Modified Rice Starches
Rice starch was cross-linked with epichlorohydrin (0.3%, w/w, on a dry starch basis) and oxidized with sodium hypochlorite (2.5% w/w), respectively. Two dual-modified rice starch samples (oxidized cross-linked rice starch and cross-linked oxidized rice ...
Feng-Xiang Yu+3 more
doaj +1 more source
Novel microarrays for simultaneous serodiagnosis of multiple antiviral antibodies. [PDF]
We developed an automated diagnostic system for the detection of virus-specific immunoglobulin Gs (IgGs) that was based on a microarray platform. We compared efficacies of our automated system with conventional enzyme immunoassays (EIAs).
Ponnurengam Malliappan Sivakumar+5 more
doaj +1 more source