Results 21 to 30 of about 40,389 (237)

Shape Transformations of Lipid Vesicles by Insertion of Bulky-Head Lipids. [PDF]

PLoS ONE, 2015
Lipid vesicles, in particular Giant Unilamellar Vesicles (GUVs), have been increasingly important as compartments of artificial cells to reconstruct living cell-like systems in a bottom-up fashion.
Soichiro Tsuda, Tatsuya Sakakura, Satoshi Fujii, Hiroaki Suzuki, Tetsuya Yomo   +4 more
doaj   +6 more sources

Visualization of membrane loss during the shrinkage of giant vesicles under electropulsation [PDF]

Biophysical Journal 96, pp 4109-4121 (2009), 2008
We study the effect of permeabilizing electric fields applied to two different types of giant unilamellar vesicles, the first formed from EggPC lipids and the second formed from DOPC lipids. Experiments on vesicles of both lipid types show a decrease in vesicle radius which is interpreted as being due to lipid loss during the permeabilization process ...
Abidor, Abidor, Abidor, Angelova, Antov, Barnett, Belehradek, Carvalho, Crowley, Cyril Favard, David S. Dean, Derjaguin, Dimova, Escoffre, Favard, Franc Camps i Febrer, Gabriel, Gehl, Gilbert, Glaser, Golzio, Golzio, Gothelf, Hu, Hu, Isambert, Israelachvili, Jean-Michel Escoffre, Khalifat, Kinosita, Kummrow, Leontiadou, Marie-Pierre Rols, Marszalek, Needham, Neu, Neu, Neumann, Neumann, Pasenkiewicz-Gierula, Pastushenko, Pavlin, Powell, Riske, Riske, Robello, Rodriguez, Rodriguez, Rols, Rols, Sens, Sersa, Sugàr, Tarek, Teissié, Teissié, Tekle, Thomas Portet, Tieleman, Tieleman, Tsong, Weaver, Weaver, Winterhalter, Wohlert   +64 more
arxiv   +8 more sources

Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles [PDF]

Colloids Surf B 64 (2008) 77-87, 2007
A recently described technique (Estes and Mayer, Biochim. Biophys. Acta 1712 (2005) 152--160) for the preparation of giant unilamellar vesicles (GUVs) in solutions with high ionic strength is examined. By observing a series of osmotic swellings followed by vesicle bursts upon a micropipette transfer of a single POPC GUV from a sucrose solution into an ...
Akashi, Angelova, Angelova, Angelova, Angelova, Bagatolli, Bloom, Božič, Bresseleers, Deamer, Dimova, D’Errico, Estes, Estes, Estes, Evans, Heinrich, Hope, Koslov, Kralj-Iglič, Kuribayashi, Mally, Mally, Mathivet, Morse, Moscho, Needham, Okumura, Olbrich, Orbach, Paula, Paula, Primož Peterlin, Redelmeier, Reeves, Riske, Rodriguez, Taylor, Ternström, Vesna Arrigler, Yamashita   +40 more
arxiv   +3 more sources

Effects of membrane potentials on the electroporation of giant unilamellar vesicles. [PDF]

PLoS ONE, 2023
Living organisms maintain a resting membrane potential, which plays an important role in various biophysical and biological processes. In the context of medical applications, irreversible electroporation (IRE) is a non-thermal and minimally invasive ...
Md Abdul Wadud, Mohammad Abu Sayem Karal, Md Moniruzzaman, Md Mamun Or Rashid   +3 more
doaj   +2 more sources

Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics [PDF]

Communications Chemistry, 2021
The production of giant unilamellar vesicles by microfluidics commonly involves additives, which may interfere with the resultant membrane properties. Here pure lipid GUVs are prepared which exclude residual surfactants and other additives.
Naresh Yandrapalli, Julien Petit, Oliver Bäumchen, Tom Robinson   +3 more
doaj   +2 more sources

A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets [PDF]

Membranes, 2023
Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells.
Yiting Zhang, Haruto Obuchi, Taro Toyota
doaj   +2 more sources

Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network [PDF]

BMC Bioinformatics, 2022
Background Fluorescence image analysis in biochemical science often involves the complex tasks of identifying samples for analysis and calculating the desired information from the intensity traces.
Il-Hyung Lee, Sam Passaro, Selin Ozturk, Juan Ureña, Weitian Wang   +4 more
doaj   +2 more sources

Functional reconstitution of plant plasma membrane H+-ATPase into giant unilamellar vesicles [PDF]

Scientific Reports
Membrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Huriye D. Uzun, Ekaterina Malysenko, Bo H. Justesen, Thomas Günther Pomorski   +3 more
doaj   +2 more sources

Rotaxanes with a photoresponsive macrocycle modulate the lipid bilayers of large and giant unilamellar vesicles [PDF]

Communications Chemistry
Rotaxanes equipped with actuators hold great potential for developing highly functional molecular machines. Such systems could significantly enhance our ability to study and manipulate biological and artificial membranes.
Udyogi N. K. Conthagamage, Rajitha Rajeshwar T, Stijn van der Ham, Nasim Akhtar, Macallister L. Davis, Senuri G. Jayawardana, Lilia Lopez, Hanumantha Rao Vutukuri, Jeremy C. Smith, Micholas Dean Smith, Víctor García-López   +10 more
doaj   +2 more sources

Femtoliter Injection of ESCRT-III Proteins into Adhered Giant Unilamellar Vesicles [PDF]

Bio-Protocol, 2022
The endosomal sorting complex required for transport (ESCRT) machinery mediates membrane fission reactions that exhibit a different topology from that observed in clathrin-coated vesicles.
Vasil Georgiev, Yunuen Avalos-Padilla, Xavier Fernàndez-Busquets, Rumiana Dimova   +3 more
doaj   +2 more sources