Results 21 to 30 of about 12,701 (197)

Measuring Encapsulation Efficiency in Cell-Mimicking Giant Unilamellar Vesicles. [PDF]

ACS Synth Biol, 2023
One of the main drivers within the field of bottom-up synthetic biology is to develop artificial chemical machines, perhaps even living systems, that have programmable functionality. Numerous toolkits exist to generate giant unilamellar vesicle-based artificial cells.
Supramaniam P, Wang Z, Chatzimichail S, Parperis C, Kumar A, Ho V, Ces O, Salehi-Reyhani A.   +7 more
europepmc   +5 more sources

Bulk Self-Assembly of Giant, Unilamellar Vesicles. [PDF]

ACS Nano, 2020
The desire to create cell-like models for fundamental science and applications has spurred extensive effort towards creating giant unilamellar vesicles (GUVs). However, a route to selectively self-assemble GUVs in bulk has remained elusive. In bulk solution, membrane-forming molecules such as phospholipids, single-tailed surfactants, and block ...
Kindt JT, Szostak JW, Wang A.
europepmc   +5 more sources

Hierarchical unilamellar vesicles of controlled compositional heterogeneity. [PDF]

PLoS ONE, 2012
Eukaryotic life contains hierarchical vesicular architectures (i.e. organelles) that are crucial for material production and trafficking, information storage and access, as well as energy production. In order to perform specific tasks, these compartments
Maik Hadorn, Eva Boenzli, Peter Eggenberger Hotz, Martin M Hanczyc   +3 more
doaj   +1 more source

Asymmetric Giant Unilamellar Vesicles [PDF]

Biophysical Journal, 2010
The lipid composition of the eukaryotic plasma membrane is asymmetric; that is, if the bilayer is considered to consist of two leaflets, the outer-facing leaflet contains different lipids at different concentrations than the inner-facing leaflet. While there has been much speculation as to the physiological purpose of this asymmetry, it has been ...
Hu, Peichi C., Malmstadt, Noah
openaire   +1 more source

Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining

Bio-Protocol, 2023
Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion.
Julia Baum, Huriye Uzun, Thomas Günther Pomorski   +2 more
doaj   +1 more source

Gel-Assisted Formation of Giant Unilamellar Vesicles [PDF]

Biophysical Journal, 2013
Giant unilamellar vesicles or GUVs are systems of choice as biomimetic models of cellular membranes. Although a variety of procedures exist for making single walled vesicles of tens of microns in size, the range of lipid compositions that can be used to grow GUVs by the conventional methods is quite limited, and many of the available methods involve ...
Weinberger, Andreas, Tsai, Feng-Ching, Koenderink, Gijsje H., Schmidt, Thais F., Itri, Rosângela, Meier, Wolfgang, Schmatko, Tatiana, Schröder, André, Marques, Carlos   +8 more
openaire   +3 more sources

Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid [PDF]

, 2013
Caspase-8 is involved in death receptor-mediated apoptosis in type II cells, the proapoptotic programme of which is triggered by truncated Bid. Indeed, caspase-8 and Bid are the known intermediates of this signalling pathway.
AH Stegh, AH Stegh, AJ Garcia-Saez, AJ Garcia-Saez, AM Chinnaiyan, Ana-Jesus García-Sáez, B Apellaniz, B Leber, Beate Klösgen, Boris Zhivotovsky, C Scaffidi, D Ardail, D Ardail, D Chandra, D Marsh, D Marsh, D Needham, DW Chang, E Evans, E Krieghoff, E van den Brink-van der Laan, EE Varfolomeev, Eyal Gottlieb, F Gonzalvez, F Gonzalvez, F Gonzalvez, F Gonzalvez, FC Kischkel, François Gonzalvez, G Kroemer, GC Shearman, H Zhang, HR Stennicke, J Zha, Jesus Ayala-Sanmartin, JF Lovell, JH Li, JP Medema, K Milovic-Holm, KH Lee, KM Boatright, L Mathivet, Liberty François-Moutal, M Lamblet, M Lutter, M Lutter, M Muzio, M Muzio, MA Hughes, Mark Perry, MI Angelova, MO Hengartner, MP Boldin, N Keller, Olivier Jalmar, P Juo, P Ratanabanangkoon, Patrice X. Petit, Petra Schwille, PJ Jost, RF Epand, S Zhang, T Granjon, T Kuwana, T Parasassi, Thierry Granjon, VA Torres, VA Torres, W Helfrich, W Rawicz, WC Yeh, X Luo, Y Zaltsman, ZT Schug   +73 more
core   +9 more sources

Chemical communication at the synthetic cell/living cell interface

Communications Chemistry, 2021
Synthetic models of cells are becoming increasingly sophisticated, but engineering communication between these and living cells remains challenging. Here the authors review modes of communication and signal processing between living cells and synthetic ...
Vincent Mukwaya, Stephen Mann, Hongjing Dou   +2 more
doaj   +1 more source

Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells

Nature Communications, 2021
Controlled actuation is an important aspect of synthetic cellular systems. Here, the authors combine pH responsive DNA origami structures with light triggered proton pump engineered E.
Kevin Jahnke, Noah Ritzmann, Julius Fichtler, Anna Nitschke, Yannik Dreher, Tobias Abele, Götz Hofhaus, Ilia Platzman, Rasmus R. Schröder, Daniel J. Müller, Joachim P. Spatz, Kerstin Göpfrich   +11 more
doaj   +1 more source

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles [PDF]

Journal of Visualized Experiments, 2017
The reshaping of the cell membrane is an integral part of many cellular phenomena, such as endocytosis, trafficking, the formation of filopodia, etc. Many different proteins associate with curved membranes because of their ability to sense or induce membrane curvature.
Prévost, Coline, Tsai, Feng-Ching, Bassereau, Patricia, Simunovic, Mijo   +3 more
openaire   +2 more sources